A5066628190- Force Microscopy Techniques and Applications
- Bacterial biofilms and quorum sensing
- Mechanical and Optical Resonators
- Biochemical and Structural Characterization
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Polymer Surface Interaction Studies
- Antimicrobial Resistance in Staphylococcus
- Molecular Junctions and Nanostructures
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Antifungal resistance and susceptibility
- Streptococcal Infections and Treatments
- Near-Field Optical Microscopy
- Microfluidic and Bio-sensing Technologies
- Antimicrobial Peptides and Activities
- Advanced Electron Microscopy Techniques and Applications
- Adhesion, Friction, and Surface Interactions
- Cellular transport and secretion
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Alzheimer's disease research and treatments
- Nanofabrication and Lithography Techniques
UCLouvain
2015-2024
University of Baltimore
2024
University of Maryland, Baltimore
1997-2024
VIB-KU Leuven Center for Cancer Biology
2024
KU Leuven
2006-2024
PRG S&Tech (South Korea)
2023
Walloon Excellence in Lifesciences and Biotechnology
2015-2022
Institute of Life Sciences
2014-2017
Interface (United States)
2016
Wageningen University & Research
2016
AD (Alzheimer's disease) is linked to Abeta (amyloid beta-peptide) misfolding. Studies demonstrate that the level of soluble oligomeric forms correlates better with progression disease than fibrillar forms. Conformation-dependent antibodies have been developed detect either oligomers or fibrils, suggesting structural differences between these exist. Using conditions which yield well-defined Abeta-(1-42) we studied secondary structure species by ATR (attenuated total reflection)-FTIR...
Single microbial cells can show important local variations of elasticity due to the complex, anisotropic composition their walls. An example this is yeast during cell division, where chitin known accumulate in localized region wall involved budding. We used atomic force microscopy (AFM) measure quantitatively mechanical properties hydrated cells. Topographic images and spatially resolved maps revealed significant lateral across surface, bud scar being significantly stiffer than surrounding...
To evaluate the influence of substratum surface characteristics on protein adsorption processes, we have investigated (adsorbed amount, supramolecular organization) collagen model substrata exhibiting controlled topography and chemistry. Substrata were prepared in two steps: (i) gold deposition onto silicon wafers (smooth substrata) a support with nanoscale protrusions created by colloidal lithography (rough substrata); (ii) functionalization CH3 (hydrophobic) OH (hydrophilic) groups, using...
Cell surface polysaccharides have an established role as virulence factors in human bacterial pathogens. Less documented are the biosynthesis and biological functions of beneficial bacteria. We identified a gene cluster that encodes enzymes regulatory transporter proteins for different steps extracellular (EPS) well-documented probiotic strain Lactobacillus rhamnosus GG. Subsequent mutation welE gene, encoding priming glycosyltransferase within this cluster, comparative phenotypic analyses...
Mixed distearoylphosphatidylethanolamine (DSPE) and dioleoylphosphatidylethanolamine (DOPE) monolayers bilayers have been deposited on mica using the Langmuir−Blodgett (LB) technique, as a model system for biomembranes. Investigation with atomic force microscopy revealed phase-separation both in air water form of microscopic DSPE domains embedded DOPE matrix. For air, step height measured between higher phase lower was larger than expected from molecular lengths, significant contrast...
Understanding how cell adhesion proteins form domains is a key challenge in biology. Here, we use single-molecule atomic force microscopy (AFM) to demonstrate the force-induced formation and propagation of nanodomains living fungal cells, focusing on covalently anchored cell-wall protein Als5p from Candida albicans . We show that pulling single adhesins with AFM tips terminated specific antibodies triggers 100–500 nm propagate over entire surface. Control experiments (with cells lacking...