A5071387345- Force Microscopy Techniques and Applications
- Mechanical and Optical Resonators
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Bacterial biofilms and quorum sensing
- Virus-based gene therapy research
- Biochemical and Structural Characterization
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Antifungal resistance and susceptibility
- Advanced Electron Microscopy Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Molecular Junctions and Nanostructures
- thermodynamics and calorimetric analyses
- Microfluidic and Bio-sensing Technologies
- Polymer Surface Interaction Studies
- Receptor Mechanisms and Signaling
- Blood properties and coagulation
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Integrated Circuits and Semiconductor Failure Analysis
- Wnt/β-catenin signaling in development and cancer
Walloon Excellence in Lifesciences and Biotechnology
2017-2025
UCLouvain
2015-2024
Children's Hospital of Pittsburgh
2023
University of Pittsburgh Medical Center
2023
Institute of Life Sciences
2017-2018
PRG S&Tech (South Korea)
2018
United States Virgin Islands Department of Health
2018
Institut des Arts de Diffusion
2018
Board of the Swiss Federal Institutes of Technology
2015-2016
ETH Zurich
2014-2016
Abstract Study of the interactions established between viral glycoproteins and their host receptors is critical importance for a better understanding virus entry into cells. The novel coronavirus SARS-CoV-2 cells mediated by its spike glycoprotein (S-glycoprotein), angiotensin-converting enzyme 2 (ACE2) has been identified as cellular receptor. Here, we use atomic force microscopy to investigate mechanisms which S-glycoprotein binds ACE2 We demonstrate, both on model surfaces living cells,...
Wnt signaling is key to many developmental, physiological, and disease processes in which cells seem able discriminate between multiple ligands. This selective recognition or "decoding" capacity has remained enigmatic because Wnt/Frizzled interactions are largely incompatible with monospecific recognition. Gpr124 Reck enable brain endothelial selectively respond Wnt7. We show that binds low micromolar affinity the intrinsically disordered linker region of Availability Reck-bound Wnt7 for...
Understanding how cell adhesion proteins form domains is a key challenge in biology. Here, we use single-molecule atomic force microscopy (AFM) to demonstrate the force-induced formation and propagation of nanodomains living fungal cells, focusing on covalently anchored cell-wall protein Als5p from Candida albicans . We show that pulling single adhesins with AFM tips terminated specific antibodies triggers 100–500 nm propagate over entire surface. Control experiments (with cells lacking...
Although hydrophobic forces are of great relevance in biological systems, quantifying these on complex biosurfaces such as cell surfaces has been difficult owing to the lack appropriate, ultrasensitive force probes. Here, chemical microscopy (CFM) with tips was used measure local organic and live bacteria. On surfaces, we found an excellent correlation between nanoscale CFM macroscale wettability measurements, demonstrating sensitivity method toward hydrophobicity providing novel insight...
The nanoscale exploration of microbes using atomic force microscopy (AFM) is an exciting, rapidly evolving research field. Here, we show that single-molecule spectroscopy a valuable tool for the localization and conformational analysis individual polysaccharides on live bacteria. We focus clinically important probiotic bacterium Lactobacillus rhamnosus GG, demonstrating power AFM to reveal coexistence polysaccharide chains different nature cell surface. Applicable wide variety cells, this...
Traditionally, cell surface properties have been difficult to study at the subcellular level, especially on hydrated, live cells. Here, we demonstrate ability of chemical force microscopy map hydrophobicity single cells with nanoscale resolution. After validating technique reference surfaces known chemistry, probe local hydrophobic character two medically important microorganisms, Aspergillus fumigatus and Mycobacterium bovis, in relation function. Applicable a wide variety cells, chemically...
Single-cell force spectroscopy is a powerful atomic microscopy modality in which single living cell attached to the cantilever quantify forces that drive cell-cell and cell-substrate interactions. Although various single-cell protocols are well established for animal cells, application of method individual bacterial cells remains challenging, mainly owing lack appropriate methods controlled attachment live on cantilevers. We present nondestructive protocol single-bacterial spectroscopy,...
Knowledge of the mechanisms by which bacterial pili adhere to host cells and withstand external forces is critical our understanding their functional roles offers exciting avenues in biomedicine for controlling adhesion pathogens probiotics. While much progress has been made nanoscale characterization from Gram-negative bacteria, adhesive mechanical properties Gram-positive remain largely unknown. Here, we use single-molecule atomic force microscopy unravel binding mechanism probiotic...
Currently, there is a growing need for methods that can quantify and map the molecular interactions of biological samples, both with high-force sensitivity high spatial resolution. Force–volume imaging valuable atomic force microscopy (AFM) modality probing specific sites on biosurfaces. However, low speed poor resolution this method have severely hampered its widespread use in life science research. We novel AFM mode (i.e., peak tapping chemically functionalized tips) to probe localization...
Cell adhesion molecules in Saccharomyces cerevisiae and Candida albicans contain amyloid-forming sequences that are highly conserved. We have now used site-specific mutagenesis specific peptide perturbants to explore amyloid-dependent activity the adhesin Als5p. A V326N substitution region conserved secondary structure ligand binding, but abrogated formation of amyloid fibrils soluble Als5p reduced cell surface thioflavin T fluorescence. When displayed on surface, with this prevented...
Abstract While several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM ( A ctivated Leukocyte C ell dhesion M olecule) is a cargo. We show endophilin-A3—but neither A1 nor A2 isoforms—functionally associates with CD166-containing early carriers and physically interacts Our data further demonstrates three...