A5006647926- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Signaling Pathways in Disease
- RNA Interference and Gene Delivery
- Cell Image Analysis Techniques
- Peptidase Inhibition and Analysis
- Retinal Development and Disorders
- Advanced biosensing and bioanalysis techniques
- Microtubule and mitosis dynamics
- Erythrocyte Function and Pathophysiology
- Caveolin-1 and cellular processes
- Optical Coherence Tomography Applications
- Inflammatory mediators and NSAID effects
- Endoplasmic Reticulum Stress and Disease
- Phytochemistry and Bioactive Compounds
- Mitochondrial Function and Pathology
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Melanoma and MAPK Pathways
- Carbohydrate Chemistry and Synthesis
- Sphingolipid Metabolism and Signaling
Inserm
2014-2025
Institut Curie
2015-2025
Université Paris Sciences et Lettres
2016-2025
Université de Rennes
2025
Centre National de la Recherche Scientifique
2011-2024
Chimie et Biologie de la Cellule
2017-2023
Robert Bosch Hospital
2023
Chimie et Biologie des Membranes et des Nanoobjects
2016-2020
RELX Group (United States)
2019
Universitätsklinikum Würzburg
2016
Mitochondria undergo fission–fusion events that render these organelles highly dynamic in cells. We report a relationship between mitochondrial form and cell cycle control at the G 1 –S boundary. convert from isolated, fragmented elements into hyperfused, giant network transition. The is electrically continuous has greater ATP output than mitochondria any other stage. Depolarizing early to prevent changes causes progression S phase be blocked. Inducing hyperfusion by acute inhibition of...
Microvascular endothelial barrier breakdown in sepsis precedes organ failure and death patients. We tested the hypothesis that formation of endothelium-derived soluble vascular (VE)-cadherin fragments (sVE-cadherin) is involved inflammation-induced disruption. Incubation human dermal microvascular cells (HDMEC) with tumour necrosis factor-α (TNF-α) bacterial lipopolysaccharide (LPS) led to disruption which correlated significantly increased sVE-cadherin at a size ∼90 kDa cell culture...
Abstract While several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM ( A ctivated Leukocyte C ell dhesion M olecule) is a cargo. We show endophilin-A3—but neither A1 nor A2 isoforms—functionally associates with CD166-containing early carriers and physically interacts Our data further demonstrates three...
Ludger Johannes1,2, Christian Wunder1,2 and Patricia Bassereau3,4,5 1Institut Curie—Centre de Recherche, Traffic, Signaling Delivery Group, 75248 Paris Cedex 05, France 2CNRS UMR144, 3Institut Membrane Cell Functions 4CNRS UMR168, 5Université Pierre et Marie Curie, F-75252 Paris, Correspondence: ludger.johannes{at}curie.fr
Abstract Gangliosides in the outer leaflet of plasma membrane eukaryotic cells are essential for many cellular functions and pathogenic interactions. How gangliosides dynamically organized how they respond to ligand binding is poorly understood. Using fluorescence anisotropy imaging synthetic, fluorescently labeled GM1 incorporated into living cells, we found that with a fully saturated C16:0 acyl chain, but not unsaturated C16:1 actively clustered nanodomains, which depends on cholesterol,...
O-Glycans of the human gastric mucosa show antimicrobial activity against pathogenic bacterium Helicobacter pylori by inhibiting bacterial cholesterol-alpha-glucosyltransferase (Kawakubo, M., Ito, Y., Okimura, Kobayashi, Sakura, K., Kasama, S., Fukuda, M. N., Katsuyama, T., and Nakayama, J. (2004) Science 305, 1003-1006). This enzyme catalyzes first step in biosynthesis four unusual glycolipids: cholesteryl-alpha-glucoside, cholesteryl-6'-O-acyl-alpha-glucoside,...
Cyclooxygenase-2 (COX-2) represents the inducible key enzyme of arachidonic acid metabolism and contributes to pathogenesis gastroduodenal ulcers gastric cancer. Helicobacter pylori infection is associated with elevated COX-2 levels, but mechanisms underlying H. pylori-dependent cox-2 gene expression are unclear. stimulated mRNA protein abundance in epithelial cells vitro vivo, functional analysis promoter mapped its pylori-responsive region a proximal CRE/Ebox element at -56 -48. Moreover,...
VEGF-A is a key regulator of inflammatory and tumor-associated angiogenesis. H. pylori plays critical role in the pathogenesis benign malignant gastric diseases. It has been suggested that infection associated with activation host angiogenesis, however, underlying mechanisms as well angiogenic growth factors activated by bacterium have not yet identified. Therefore, we investigated influence on candidate target gene vivo vitro. We show potently up-regulates production release protein vegf-A...
Access to defined glycans and glycoconjugates is pivotal for discovery, dissection, harnessing of a range biological functions orchestrated by cellular glycosylation processes the glycome. We previously employed genetic glycoengineering nuclease-based gene editing develop sustainable production designer glycoprotein therapeutics cell-based glycan arrays that display in their natural context at cell surface. However, access human formats quantities allow structural studies molecular...
To elucidate the physiologic function of renal globotriaosylceramide (Gb3/CD77), which up-to-date has been associated exclusively with Shiga toxin binding, we have analyzed in Gb3-deficient mice. Gb3 synthase KO (Gb3S-/-) mice displayed an increased albumin and low molecular weight protein excretion compared to WT. localized at brush border within vesicular structures WT proximal tubules now shown be closely receptor complex megalin/cubilin uptake. In two clinically relevant mouse models...
Abstract Recent advances in the field demonstrate high diversity and complexity of endocytic pathways. In current study, we focus on endocytosis L1CAM. This glycoprotein plays a major role development nervous system, is involved cancer associated with metastases poor prognosis. Two L1CAM isoforms are subject to endocytosis: isoform 1, described as clathrin‐mediated cargo; 2, whose has never been studied. Deciphering molecular machinery 2 internalisation should contribute better understanding...
Membrane-bound glucocorticoid receptors (mGCR) are up regulated on monocytes after in vitro stimulation and patients with rheumatoid arthritis. Caveolin-1 is critical for the transport of plasma membrane oestrogen to cell surface.To investigate expression mGCR systemic lupus erythematosus (SLE)-a disease different aetiopathogenesis treatment regimens-and examine whether caveolin-1 surface.Frequencies mGCR+ peripheral blood mononuclear cells were measured using high-sensitivity...