A5082107271- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
- Alzheimer's disease research and treatments
- Microtubule and mitosis dynamics
- Retinal Development and Disorders
- Computational Drug Discovery Methods
- Photosynthetic Processes and Mechanisms
- Bacterial Genetics and Biotechnology
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Nanopore and Nanochannel Transport Studies
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Plant and Biological Electrophysiology Studies
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- Calcium signaling and nucleotide metabolism
- Plant Molecular Biology Research
- Diffusion and Search Dynamics
- Genomics and Chromatin Dynamics
EMBL Australia
2017-2024
Navajo Technical University
2024
Monash University
2018-2024
UNSW Sydney
2017-2024
ARC Centre of Excellence in Advanced Molecular Imaging
2017-2024
Discovery Institute
2018-2024
Virginia Tech
2024
Australian Regenerative Medicine Institute
2021-2024
Institute of Genomics and Integrative Biology
2024
Australian Research Council
2022
The bacterial Shiga toxin interacts with its cellular receptor, the glycosphingolipid globotriaosylceramide (Gb3 or CD77), as a first step to entering target cells. Previous studies have shown that molecules cluster on plasma membrane, despite apparent lack of direct interactions between them. precise mechanism by which this clustering occurs remains poorly defined. Here, we used vesicle and cell systems computer simulations show line tension due curvature, height, compositional mismatch,...
Abstract cGAS‐STING signalling is induced by detection of foreign or mislocalised host double‐stranded (ds)DNA within the cytosol. STING acts as major hub, where it controls production type I interferons and inflammatory cytokines. Basally, resides on ER membrane. Following activation traffics to Golgi initiate downstream subsequently endolysosomal compartments for degradation termination signalling. While known be degraded lysosomes, mechanisms controlling its delivery remain poorly...
In Escherichia coli, a contractile ring (Z-ring) is formed at midcell before cytokinesis. This consists primarily of FtsZ, tubulin-like GTPase, that assembles into protofilaments similar to those in microtubules but different their suprastructures. The Min proteins MinC, MinD, and MinE are determinants Z-ring positioning E. coli. MinD oscillate from pole pole, genetic biochemical evidence concludes MinC positions the by coupling its assembly oscillations direct inhibitory interaction....
Abstract Gangliosides in the outer leaflet of plasma membrane eukaryotic cells are essential for many cellular functions and pathogenic interactions. How gangliosides dynamically organized how they respond to ligand binding is poorly understood. Using fluorescence anisotropy imaging synthetic, fluorescently labeled GM1 incorporated into living cells, we found that with a fully saturated C16:0 acyl chain, but not unsaturated C16:1 actively clustered nanodomains, which depends on cholesterol,...
Abstract Autophagy depends on the repopulation of lysosomes to degrade intracellular components and recycle nutrients. How cells co‐ordinate lysosome during basal autophagy, which occurs constitutively under nutrient‐rich conditions, is unknown. Here, we identify an endosome‐dependent phosphoinositide pathway that links PI3Kα signaling autophagy. We show PI3Kα‐derived PI(3)P generated by INPP4B late endosomes was required for but not starvation‐induced autophagic degradation. signals were...
Volumetric, sub-micron to micron level resolution imaging is necessary assay phenotypes or characteristics at the sub-cellular/organelle scale. However, three-dimensional fluorescence of cells typically low throughput compromises on achievable in space and time. Here, we capitalise flow control capabilities microfluidics combine it with microoptics integrate light-sheet based directly into a microfluidic chip. Our optofluidic system flows suspended through sub-micrometer thick formed using...
The phosphoinositidyl lipid (PIP) family is key to spatial regulation of cellular processes, and their appearance, disappearance, interconversion provide signals for membrane remodeling at the plasma beyond. These are connected recruitment various peripheral proteins, including sorting nexin 9 (SNX9), an important regulator late stage endocytic macropinocytic remodeling. Using a combination live cell imaging, in vitro reconstitution, atomistic molecular dynamics simulation, we probe question...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe nigericin-mediated transport of sodium and potassium ions through phospholipid bilayers studied by sodium-23 potassium-39 NMR spectroscopyFrank G. Riddell, S. Arumugam, Peter J. Brophy, Brian Cox, Mary C. H. Payne, Timothy E. SouthonCite this: Am. Chem. Soc. 1988, 110, 3, 734–738Publication Date (Print):February 1988Publication History Published online1 May 2002Published inissue 3 February...
Bending over backward: Despite their small size, bacteria display highly organized cytoskeletal structures. Using microfabricated supports for model membranes, mechanical features of FtsZ (blue hexagons) filaments, a key component bacterial cell division, can be addressed. Studying the curvature an filament into groove or around capillary (see picture) helps to understand its mechanics.
ABSTRACT Bacterial cell division initiates with the formation of a ring-like structure at center composed tubulin homolog FtsZ (the Z-ring), which acts as scaffold for assembly complex, divisome. Previous studies have suggested that divisome is initially polymers stabilized by membrane anchors FtsA and ZipA, then recruit remaining proteins. The MinCDE proteins prevent Z-ring poles oscillating from pole to pole, thereby ensuring concentration inhibitor, MinC, lowest center. We show prior...
Abstract Endosomal maturation is critical for robust and timely cargo transport to specific cellular compartments. The most prominent model of early endosomal involves a phosphoinositide-driven gain or loss proteins on individual endosomes, emphasising an autonomous stochastic description. However, limitations in fast, volumetric imaging long hindered direct whole cell-level measurements absolute numbers events. Here, we use lattice light-sheet bespoke automated analysis track very...
Recent experiments on monoaminergic neurons have shown that neurotransmission can originate from somatic release. However, little is known about the quantity of monoamine available to be released through this extrasynaptic pathway or intracellular dynamics mediate such Using three-photon microscopy, we directly imaged serotonin autofluorescence and investigated total content, release competence, kinetics serotonergic vesicles in dorsal raphe rat. We found somata primary cultured contain a...
Epigenetic modifications can maintain or alter the inherent symmetry of nucleosome. However, mechanisms that deposit and/or propagate asymmetry are not understood. Here we report yeast Set1C/COMPASS (complex proteins associated with Set1) is dimeric and, consequently, symmetrically trimethylates histone 3 Lys4 (H3K4me3) on promoter nucleosomes. Mutation dimer interface to make Set1C monomeric abolished H3K4me3 most promoters. The active promoters, particularly those involved in oxidative...
Stapled peptides have great potential as modulators of protein–protein interactions (PPIs). However, there is a vast landscape chemical features that can be varied for any given peptide, and identifying set maximizes cellular uptake subsequent target engagement remains key challenge. Herein, we present systematic analysis staple functionality on the peptide bioactivity in assays. Through application "toolbox" diversified dialkynyl linkers to stapling MDM2-binding via double-click approach,...
The understanding of lipid membranes and their organization has undergone significant development with better techniques therefore more resolved experiments. Many new factors organizing principles have been discovered, interplay between these is expected to result in rich functional behaviours. major regulating the lateral membrane heterogeneity, apart from well-studied phase separation, are cytoskeleton pinning, clustering lipids curvature. These effective means create domains that provide...
Functional regulation of proteins is central to living organisms. Here it shown that a nonfunctional conformational state polypeptide can be kinetically trapped in lipid bilayer environment. This metastable structure stable for weeks just above the phase transition temperature lipid. When samples are incubated several days at 68 degrees C, 50% conformation converts minimum-energy functional state. result suggests possibility another mechanism protein activity may available membrane proteins:...