In isolation from their peers, Photinus carolinus fireflies flash with no intrinsic period between successive bursts. Yet, when congregating into large mating swarms, these transition predictability, synchronizing neighbors a rhythmic periodicity. Here we propose mechanism for emergence of synchrony and periodicity, formulate the principle in mathematical framework. Remarkably, fitting parameters, analytic predictions this simple framework agree strikingly well data. Next, add further...

Our understanding of the bacterial cell cycle is framed largely by population-based experiments that focus on behavior idealized average cells. Most famously, contributions Cooper and Helmstetter help to contextualize phenomenon overlapping replication cycles observed in rapidly growing bacteria. Despite undeniable value these approaches, their necessary reliance cells masks stochasticity inherent single-cell growth physiology limits mechanistic value. To bridge this gap, we propose an...

Abstract Endosomal maturation is critical for robust and timely cargo transport to specific cellular compartments. The most prominent model of early endosomal involves a phosphoinositide-driven gain or loss proteins on individual endosomes, emphasising an autonomous stochastic description. However, limitations in fast, volumetric imaging long hindered direct whole cell-level measurements absolute numbers events. Here, we use lattice light-sheet bespoke automated analysis track very...

Microfluidic platforms enable long-term quantification of stochastic behaviors individual bacterial cells under precisely controlled growth conditions. Yet, quantitative comparisons physiological parameters and cell different microorganisms in experimental device modalities is not available due to experiment-specific details affecting physiology. To rigorously assess the effects mechanical confinement, we designed, engineered, performed side-by-side experiments otherwise identical conditions...

How do complex systems maintain key emergent “state variables” at desired target values to within specified tolerances? This question was first posed in the context of homeostasis living over a century ago, and yet precise quantitative rules governing this phenomenon have remained fiercely debated. We herein present direct solution through synthesis high-precision experiments principles-based physics theory. After introducing general approach that incorporates inherently stochastic dynamic...

Abstract Individual bacterial cells grow and divide stochastically. Yet they maintain their characteristic sizes across generations within a tightly controlled range. What rules ensure intergenerational stochastic homeostasis of individual cell sizes? Valuable clues have emerged from high-precision longterm tracking statistically-identical Caulobacter crescentus as reported in [1]: Intergenerational size is an inherently phenomenon, follows Markovian or memory-free dynamics, obey scaling...

Building on the known scaling law that a single timescale, cellular unit of time, governs stochastic growth and division individual bacterial cells under constant conditions, here we articulate an analogous ansatz for time-varying conditions. We propose dynamic rescaling time captures predominant effect external variations in Using this temporal ansatz, derive exact analytic results how time-dependent cell age distribution adapts to changing Our reveal natural representation these dynamics....

Do individual bacterial cells retain memories of the history environmental conditions experienced in previous generations? Here we directly address this question through a synthesis physics theory and high-precision experiments on statistically identical, non-interacting cells, which grow divide with intrinsic stochasticity precisely controlled conditions. From these data, extract “emergent simplicities” seemingly complex interplay between dependence, persistence, transience stochastic...

Living systems are naturally complex and adaptive offer unique insights into the strategies for achieving sustaining stochastic homeostasis in different conditions. Here we focus on context of growth division individual bacterial cells. We take advantage high-precision long-term dynamical data that have recently been used to extract emergent simplicities articulate empirical intra- intergenerational scaling laws governing these dynamics. From data, identify core motif mechanistic coupling...

Organisms maintain the status quo, holding key physiological variables constant to within an acceptable tolerance, and yet adapt with precision plasticity dynamic changes in externalities. What organizational principles ensure such exquisite robust control of systems-level "state variables" complex systems extraordinary number moving parts fluctuating variables? Here we focus on these issues specific context intra- intergenerational life histories individual bacterial cells, whose...

What are the signatures of onset catastrophe? Here we present rich system physics characterizing stochasticity driven transition from homeostasis to breakdown in an experimentally motivated and analytically tractable minimal model. Recent high-precision experiments on individual bacterial cells, growing dividing repeatedly a variety environments, have revealed previously unknown intergenerational scaling law which not only uniquely determines stochastic map governing homeostasis, but also,...

Organisms maintain the status quo, holding key physiological variables constant to within an acceptable tolerance, and yet adapt with precision plasticity dynamic changes in externalities. What organizational principles ensure such exquisite robust control of systems-level “state variables” complex systems extraordinary number moving parts fluctuating variables? Here, we focus on these issues specific context intra- intergenerational life histories individual bacterial cells, whose...

In the present paper, possibility of invoking stochastic resonance (SR, periodic and aperiodic) by regulating operating value an appropriate parameter is explored. The values these parameters are defined as set point system throughout paper. Brusselator, a mathematical model [I. Prigogine R. Lefever, J. Chem. Phys. 48, 1695 (1968)JCPSA60021-960610.1063/1.1668896] nonlinear chemical reactions, used for this purpose. We consider effect intrinsic noise in Brusselator due to Markovian nature...

Living systems are naturally complex and adaptive, offer unique insights into the strategies for achieving sustaining stochastic homeostasis in different conditions. Here, we focus on context of growth division individual bacterial cells. We take advantage high-precision longterm dynamical data that have recently been used to extract emergent simplicities articulate empirical intra- in-tergenerational scaling laws governing these dynamics. identify core motif mechanistic coupling between...

Abstract In isolation from their peers, Photinus carolinus fireflies flash with no intrinsic period between successive bursts. Yet, when congregating into large mating swarms, these transition predictability, synchronizing neighbors a rhythmic periodicity. Here we propose mechanism for emergence of synchrony and periodicity, formulate the principle in mathematical framework. Remarkably, fitting parameters, analytic predictions this simple framework agree strikingly well data. Next, add...

Endosomal maturation is critical for robust and timely cargo transport to specific cellular compartments. The most prominent model of early endosomal involves phosphoinositide-driven gain or loss proteins on individual endosomes, emphasising an autonomous stochastic description. However, limitations in fast, volumetric imaging long hindered direct whole-cell measurements absolute numbers events. Here, we use lattice light-sheet bespoke automated analysis track very (APPL1-positive)...

The interior of a living cell is an active, fluctuating, and crowded environment. Yet, it maintains high level coherent organization, which readily apparent in the intracellular transport network. Membrane-bound compartments called endosomes play key role carrying cargo, conjunction with myriad components including cargo adaptor proteins, membrane sculptors, motor cytoskeleton. These coordinate to effectively navigate specific locations, even though underlying protein interactions enzymatic...

Microfluidic platforms enable long-term quantification of stochastic behaviors individual bacterial cells under precisely controlled growth conditions. Yet, quantitative comparisons physiological parameters and cell different microorganisms in experimental device modalities is not readily possible owing to experiment-specific details affecting physiology confounding ways. To rigorously assess the effects mechanical confinement, we designed, engineered, performed side-by-side experiments...

Abstract Our current understanding of the bacterial cell cycle is framed largely by population-based experiments that focus on behavior idealized average cells. Most famously, contributions Cooper and Helmstetter help to contextualize phenomenon overlapping replication cycles observed in rapidly growing bacteria. Despite undeniable value these approaches, their necessary reliance cells washes out stochasticity inherent single growth physiology limiting mechanistic value. To bridge this gap,...

ABSTRACT Organisms are able to partition resources adaptively between growth and repair. The precise nature of optimal partitioning how this emerges from cellular dynamics including insurmountable trade-offs remains an open question. We construct a mathematical framework estimate the corresponding maximal rate constrained by empirical scaling laws. model biosynthesis tradeoff governing ribosome economy replicating functional proteins pool, also energy arising finite budget cell. Through...