A5066191404- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Pluripotent Stem Cells Research
- Chromosomal and Genetic Variations
- Light effects on plants
- Plant Disease Resistance and Genetics
- CRISPR and Genetic Engineering
- Genetic diversity and population structure
- Animal Behavior and Reproduction
- Cancer-related gene regulation
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Insect Utilization and Effects
- Renal and related cancers
Monash University
2016-2024
Australian Regenerative Medicine Institute
2020-2024
Abstract Cryptochromes (CRYs) are evolutionarily conserved photolyase-like photoreceptors found in almost all species, including mammals. CRYs regulate transcription by modulating the activity of several factors, but whether and how they affect pre-mRNA processing unknown. Photoperiod temperature closely associated seasonal cues that influence reproductive timing plants. mediate photoperiod-responsive floral initiation, it is largely unknown also involved thermosensory flowering. We...
The establishment and maintenance of pluripotency depend on precise coordination gene expression. We establish serine-arginine-rich splicing factor 3 (SRSF3) as an essential regulator RNAs encoding key components the mouse circuitry, SRSF3 ablation resulting in loss its overexpression enhancing reprogramming. Strikingly, binds to core transcription Nanog mRNA facilitate nucleo-cytoplasmic export independent splicing. In absence binding, is sequestered nucleus protein levels are severely...
Short tandem repeats (STRs) mutations are one of the most important fuels evolution, but often largely ignored despite their huge effects on phenotypic variations. Here, we leveraged seven Arabidopsis mutation accumulation lines to assess STR rate, which ranged from 1.93x10-2 4.40x10-3 per locus generation and was much higher than common SNP rate at level 10-9-10-8. Interspecific comparison revealed rapid turnover, with a large proportion (71.8%) only present in Arabidopsis. In addition,...
Abstract RNA splicing, and variations in this process referred to as alternative are critical aspects of gene regulation eukaryotes. From environmental responses plants being a primary link between genetic variation disease humans, splicing differences confer extensive phenotypic changes across diverse organisms (1–3). Regulation occurs through differential selection splice sites reaction, which results the abundance isoforms and/or events. However, genomic determinants that influence...
Efficient mRNA splicing is a prerequisite for protein biosynthesis and the eukaryotic machinery evolutionarily conserved among species of various phyla. At its catalytic core resides activated complex Bact consisting three small nuclear ribonucleoprotein complexes (snRNPs) U2, U5 U6 so-called NineTeen (NTC) which important spliceosomal activation. CWC15 an integral part NTC in humans it associated with other species. Here we show ubiquitous expression developmental importance Arabidopsis...
Triplet repeat expansions underlie several human genetic diseases such as Huntington's disease and Friedreich's ataxia. Although mutations are primarily known from humans, a triplet expansion associated defect has also been reported at the IIL1 locus in Bur-0 accession of model plant Arabidopsis thaliana. The is an example cryptic variation its phenotypic effects seen only under or environmental perturbation, with high temperatures resulting growth defect. Here we demonstrate that not...
In insects, many critical olfactory behaviours are mediated by the large odorant receptor ( Or ) gene family, which determines response properties of different classes neurons (ORNs). While ORN responses generally conserved within and between Drosophila species, variant alleles D. melanogaster Or22 locus have previously been shown to alter profile an class called ab3A. These show potential clinal variation, suggesting that selection is acting at this locus. Here, we investigated if changes...
RNA splicing, and variations in this process referred to as alternative are critical aspects of gene regulation eukaryotes. From environmental responses plants being a primary link between genetic variation disease humans, splicing differences confer extensive phenotypic changes across diverse organisms 1–3 . Current approaches for analysing rely on quantifying variant transcripts (i.e., isoforms) or events intron retention, exon skipping etc) 4, 5 However, occurs at the level selection...
SUMMARY Changes in splicing are observed between cells, tissues, organs, individuals, and species. These changes can mediate phenotypic variation ranging from flowering time differences plants to genetic diseases humans. However, the genomic determinants of largely unknown. Here, we quantified usage individual splice-sites uncover extensive individuals (genotypes) Arabidopsis, Drosophila Humans. We used this robust quantitative measure as a phenotype mapped splice-site using Genome-Wide...
Epigenetic gene silencing induced by expanded repeats can cause diverse phenotypes ranging from severe growth defects in plants to genetic diseases such as Friedreich’s ataxia humans 1 . The molecular mechanisms underlying repeat expansion-induced epigenetic remain largely unknown 2,3 Using a plant model, we have previously shown that induce smallRNAs which turn lead through the RNA-dependent DNA methylation pathway 4,5 Here, using suppressor screen, confirm key role for RdDM and identify...
Abstract In insects many critical olfactory behaviours are mediated by the large odorant receptor (Or) gene family, which determine response properties of different classes neurons (ORNs). While ORN responses generally conserved within and between Drosophila species, variant alleles D.melanogaster Or22 locus have previously been shown to profiles an class called ab3A. These show potential clinal variation, suggesting that selection is acting at this locus. Here, we investigated if changes...
Abstract N6 -methyladenosine (m 6 A) is the most predominant internal mRNA modification in eukaryotes, recognised by its reader proteins (so-called m A-readers) for regulating subsequent fates — splicing, export, localisation, decay, stability, and translation to control several biological processes. Although a few A-readers have been identified, yet list incomplete. Here, we identify new A-reader protein, Moloney leukaemia virus 10 homologue (MOV10), A pathway. MOV10 recognises A-containing...