A5033766217- RNA Research and Splicing
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Vitamin D Research Studies
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Platelet Disorders and Treatments
- Cancer-related Molecular Pathways
- Thyroid Disorders and Treatments
- Thyroid Cancer Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Advanced Breast Cancer Therapies
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
Monash University
2017-2023
Hudson Institute of Medical Research
2019-2023
Australian Regenerative Medicine Institute
2018-2021
Australian National University
2012-2018
Discovery Institute
2018
Gene expression is regulated through multiple steps at both transcriptional and post-transcriptional levels. The net abundance of mature mRNA species in cells determined by the balance between transcription degradation. Thus, regulation stability a key event that can greatly affect level mRNAs cells. within be measured indirectly analyzing half-life following inhibition, where changes levels are assumed to reflect Determination as measure useful understanding gene underlying mechanisms...
The establishment and maintenance of pluripotency depend on precise coordination gene expression. We establish serine-arginine-rich splicing factor 3 (SRSF3) as an essential regulator RNAs encoding key components the mouse circuitry, SRSF3 ablation resulting in loss its overexpression enhancing reprogramming. Strikingly, binds to core transcription Nanog mRNA facilitate nucleo-cytoplasmic export independent splicing. In absence binding, is sequestered nucleus protein levels are severely...
RNA processing is increasingly recognized as a critical control point in the regulation of different hematopoietic lineages including megakaryocytes responsible for production platelets. Platelets are anucleate cytoplasts that contain rich repertoire RNAs encoding proteins with essential platelet functions derived from parent megakaryocyte. It largely unknown how binding contribute to development and We show serine-arginine-rich splicing factor 3 (SRSF3) megakaryocyte maturation generation...
Abstract MicroRNA (miRNA) biogenesis is tightly regulated to maintain distinct miRNA expression patterns. Almost half of mammalian miRNAs are generated from clusters, but this process not well understood. We show here that Serine‐arginine rich splicing factor 3 (SRSF3) controls the processing miR‐17‐92 cluster in pluripotent and cancer cells. SRSF3 binding multiple CNNC motifs downstream Drosha cleavage sites within required for efficient cluster. depletion specifically compromises two...
Mammalian cells express hundreds of RNA binding proteins (RBPs) that are essential regulators metabolism. RBP activity plays a central role in the control gene expression programs and identification RNA-protein interactions is critical for comprehensive understanding regulation cells. In recent years, various tools techniques to identify these have been developed. Among those, immunoprecipitation precise powerful assay can be used establish physical interaction an individual with its target...
Abstract Almost a half of microRNAs (miRNAs) in mammalian cells are generated from polycistronic primary transcripts encoding more than one miRNA. Mature miRNAs clusters frequently regulate complementary sets target mRNAs. How the processing individual within is controlled to give rise distinct miRNA levels vivo not fully understood. Our investigation SRSF3 (Serine-Arginine Rich Splicing Factor3) regulated noncoding RNAs pluripotent identified miR-17-92 cluster as key target, binding CNNC...
Abstract Vitamin D is a group of fat-soluble secosteroids. Apart from its main physiological role in calcium homeostasis, several studies have demonstrated wide range functions vitamin which can be associated with cancer. Even though the exact mechanism underlying protective action against cancer not clearly understood, there strong experimental evidence showing that participates cell growth regulation, apoptosis and proliferation normal malignant breast cells. This has lead to hypothesis...
Two recessive mammary tumour susceptibility loci, SuprMam1 (for suppressor of tumours) on chromosome 7 (110-140 Mb) and SuprMam2 2 (120-140MB) have been identified in the BALB/c mouse strain Trp53+/- model spontaneous breast cancer [1]. We studied gland morphology expression levels potential candidate genes SM09 congenic mice (BALB/c SuprMam loci C57BL/6 background) comparison to parental strains, identify within that might be responsible for higher mice. We analysed wholemounts...