A5076150919- Zebrafish Biomedical Research Applications
- Neural dynamics and brain function
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Congenital heart defects research
- Marine animal studies overview
- Genetics and Neurodevelopmental Disorders
- Axon Guidance and Neuronal Signaling
- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Orbital Angular Momentum in Optics
- Autism Spectrum Disorder Research
- Microfluidic and Bio-sensing Technologies
- Functional Brain Connectivity Studies
- Child Development and Digital Technology
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Neuroendocrine regulation and behavior
- Developmental Biology and Gene Regulation
- Animal Vocal Communication and Behavior
- Plant Molecular Biology Research
- Connexins and lens biology
The University of Queensland
2016-2025
The University of Melbourne
2022-2025
Allen Institute for Brain Science
2024-2025
Florey Institute of Neuroscience and Mental Health
2023
Clinic for Special Children
2022
New Leaf Center
2022
Eaton (United States)
2022
Institute of Biomedical Science
2019
University of California, San Francisco
2007-2010
Queensland Eye Institute
2009
Enhancer function, from sponges to humans Identifying the function of enhancers, DNA regions that help regulate gene expression and evolve rapidly, has been difficult. This area research hampered by difficultly in identifying functional conservation. Wong et al. now show despite low sequence conservation, enhancer is strongly conserved through animal kingdom (see Perspective Harmston). Transgenic sponge enhancers zebrafish mice demonstrates these sequences can drive cell type–specific across...
The optic tectum of zebrafish is involved in behavioral responses that require the detection small objects. superficial layers tectal neuropil receive input from retinal axons, while its deeper convey processed information to premotor areas. Imaging with a genetically encoded calcium indicator revealed deep layers, as well dendrites single neurons, are preferentially activated by visual stimuli. This spatial filtering relies on GABAergic interneurons (using neurotransmitter γ-aminobutyric...
We have carried out a Gal4 enhancer trap screen in zebrafish, and generated 184 stable transgenic lines with interesting expression patterns throughout the nervous system. Of these, three display clear tectum, each distinguishable stereotyped distribution of expressing cells. Detailed morphological analysis single cells, using genetic "Golgi-like" labelling method, revealed four common cell types (superficial, periventricular, shallow radial glial), along range other less neurons. The...
Abstract Background Loss or disrupted expression of the FMR1 gene causes fragile X syndrome (FXS), most common monogenetic form autism in humans. Although disruptions sensory processing are core traits FXS and autism, neural underpinnings these phenotypes poorly understood. Using calcium imaging to record from entire brain at cellular resolution, we investigated neuronal responses visual auditory stimuli larval zebrafish, using fmr1 mutants model FXS. The purpose this study was alterations...
Abstract Since the time of their introduction, optical tweezers (OTs) have grown to be a powerful tool in hands biologists. OTs use highly focused laser light guide, manipulate, or sort target objects, typically nanoscale microscale range. been particularly useful making quantitative measurements forces acting cellular systems; they can reach inside living cells and used study mechanical properties fluids structures that contain. As all are conducted without physically contacting system...
Abstract The vestibular system, which detects gravity and motion, is crucial to survival, but the neural circuits processing information remain incompletely characterised. In part, this because movement needed stimulate system hampers traditional neuroscientific methods. Optical trapping uses focussed light apply forces targeted objects, typically ranging from nanometres a few microns across. principle, optical of otoliths (ear stones) could produce fictive stimuli in stationary animal. Here...
Abstract Habituation is a form of learning during which animals stop responding to repetitive stimuli, and deficits in habituation are characteristic several psychiatric disorders. Due technical challenges, the brain-wide networks mediating poorly understood. Here we report calcium imaging larval zebrafish repeated visual looming stimuli. We show that different functional categories loom-sensitive neurons located locations throughout brain, both properties their resulting behavior can be...
The study of dendritic development in CNS neurons has been hampered by a lack complex structures that can be studied tractable genetic system. In an effort to develop such system, we recently characterized the highly dendrites vertical system (VS) Drosophila visual Using VS as model show here using loss-of-function mutations endogenous Cdc42, member Rho family small GTPases, is required for multiple aspects morphogenesis. Cdc42-mutant display normal complexity but increased length compared...
The cerebellum is a brain region responsible for motor coordination and refining programs. While great deal known about the structure connectivity of mammalian cerebellum, fundamental questions regarding its function in behavior remain unanswered. Recently, zebrafish has emerged as useful model organism cerebellar studies, owing part to similarity circuits between mammals. cell types composing their cortical are generally conserved with mammals, lack deep nuclei, instead majority output...
Errors in clinical decision-making are disturbingly common. Here, we show that structured information–sharing networks among clinicians significantly reduce diagnostic errors, and improve treatment recommendations, as compared to groups of ...Errors Recent studies have found 10 15% all decisions regarding diagnoses inaccurate. experimentally study the ability ...