A5006320890- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- EEG and Brain-Computer Interfaces
- Circadian rhythm and melatonin
- Advanced Sensor and Energy Harvesting Materials
- Advanced Memory and Neural Computing
- Microbial Inactivation Methods
- Conducting polymers and applications
- Microfluidic and Bio-sensing Technologies
- Electrohydrodynamics and Fluid Dynamics
- Spaceflight effects on biology
- Photochromic and Fluorescence Chemistry
- Muscle activation and electromyography studies
- CRISPR and Genetic Engineering
- Electrochemical Analysis and Applications
- Retinal Development and Disorders
- Light effects on plants
- Advanced biosensing and bioanalysis techniques
- Molecular Communication and Nanonetworks
- Force Microscopy Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Olfactory and Sensory Function Studies
- Transgenic Plants and Applications
- Nanowire Synthesis and Applications
Toyohashi University of Technology
2016-2025
Juntendo University
2016
University of California, Berkeley
2005-2009
Tokyo University of Science
1998-2009
RIKEN Center for Brain Science
2009
The University of Tokyo
1998-2009
Institute of Molecular and Cell Biology
2007
In multicellular organisms, circadian oscillators are organized into multitissue systems which function as biological clocks that regulate the activities of organism in relation to environmental cycles and provide an internal temporal framework. To investigate organization a mammalian system, we constructed transgenic rat line luciferase is rhythmically expressed under control mouse Per1 promoter. Light emission from cultured suprachiasmatic nuclei (SCN) these rats was invariably robustly...
The mammalian SCN contains a biological clock that drives remarkably precise circadian rhythms in vivo and vitro. This study asks whether the cycle-to-cycle variability of behavioral mice can be attributed to precision individual pacemakers within or their interactions. authors measured standard deviation period from 7-day recordings running wheel activity, Period1 gene expression cultured explants, firing rate patterns dispersed neurons. Period intact tissue animal was lower than single...
The analysis of cell signaling requires the rapid and selective manipulation protein function. We have synthesized photoswitches that covalently modify target proteins reversibly present withdraw a ligand from its binding site due to photoisomerization an azobenzene linker. describe here properties glutamate photoswitch controls ion channel in cells. Affinity labeling geometric constraints ensure only targeted channel, enables spatial patterns light favor one location over another....
An ultrastretchable film device is developed that can follow the shape of spherical and large deformable biological samples such as heart brain tissues. Although composed biocompatible parylene for substrate metal layers platinum (Pt)/titanium (Ti), which are unstretchable materials, shows a high stretchability by patterning slits "Kirigami" design. A Pt/Ti-microelectrode array embedded in 11 µm thick with 5 × 91 exhibits strain ≈250% at 9 mN strain-force (0.08 MPa stress) Young's modulus 23...
ABSTRACT We have previously shown that endochondral ossification is finely regulated by the Clock system expressed in chondrocytes during postnatal skeletogenesis. Here we show a sophisticated modulation of bone resorption and mass through its expression bone-forming osteoblasts. Brain muscle aryl hydrocarbon receptor nuclear translocator-like protein 1 (Bmal1) Period1 (Per1) were with oscillatory rhythmicity vivo, circadian rhythm was also observed cultured osteoblasts Per1::luciferase...
Similar to neurons, microglia have an intrinsic molecular clock. The master clock oscillator Bmal1 modulates interleukin‐6 upregulation in microglial cells exposed lipopolysaccharide. can play a role inflammatory responses. We previously demonstrated that gliotransmitter ATP induces transient expression of the gene Period1 via P2X7 purinergic receptors cultured microglia. In this study, we further investigated mechanisms underlying regulation pro‐inflammatory cytokine production by molecules...
Photoswitched tethered ligands (PTLs) can be used to remotely control protein function with light. We have studied the geometric and conformational factors that determine efficacy of PTL gating in ionotropic glutamate receptor iGluR6 using a family photoiosomerizable MAG (maleimide-azobenzene-glutamate) PTLs covalently attach clamshell ligand-binding domain. Experiments molecular dynamics simulations modified proteins show optical switching depends on 2 factors: (i) relative occupancy...
The mammalian circadian system is orchestrated by a master pacemaker in the brain, but many peripheral tissues also contain independent or quasi-independent oscillators. adaptive significance of clocks these structures must lie, large part, phase relationships between constituent oscillators and their micro- macroenvironments. To examine relationship postnatal development, which dependent on endogenous programs maternal/environmental influences, oscillators, authors assessed pineal, liver,...
Electronic devices used to record biological signals are important in neuroscience, brain-machine interfaces, and medical applications. Placing electronic below the skin surface recording muscle offers accurate robust electromyography (EMG) recordings. The device stretchability flexibility must be similar tissues achieve an intimate integration of with tissues. However, conventional elastomer-based EMG electrodes have a Young's modulus that is ≈20 times higher than muscle. In addition, these...
Abstract Long‐term quantitative observations of the physiological state brain are essential technologies in various fields. In vivo electrophysiology with microelectrodes enables high spatiotemporal recordings neuronal activities; however, tissue damage occurs due to electrode penetration, which limits recording period. A neural technology is introduced that uses a 5‐µm‐diameter microneedle device. The implanted mouse cortex shows capabilities 1 day after implantation, exceeds year‐long...
Abstract Major depressive disorder (MDD) is one of the most chronic disease and life-threatening 
illnesses globally. However, mainstay medications do not provide sufficient relief for 
depressive patients with MDD. Thus, there an urgent need development analysis 
methods that allow drug screening observation phenotypes. We developed a tiny 
microneedle electrode block module called Toyohashi probe, which can be inserted into 
a mouse brain minimal...
Organisms maintain circadian rhythms corresponding to approximately 24 h in the absence of external environmental cues, and they synchronize phases their autonomous clocks light-dark cycles, feeding timing, other factors. The suprachiasmatic nucleus (SCN) occupies top position hierarchy mammalian system functions as a photic-dependent oscillator, while food-entrainable oscillator (FEO) entrains digestive peripheral tissues behaviors according timing. In mammals, neuropeptide Y (NPY) from...
We have identified novel mammalian homologues of a Drosophila clock gene, timeless , and designated them as human TIMELESS1 (h TIM1 ) mouse Timeless1 (m Tim1 ), respectively. These genes were mapped by FISH to chromosomal regions 12q12‐13 in 10D3 mouse. The deduced amino acid sequences hTim1 mTim1 proteins 1208 1197 acids length shared 83% identity. Northern blot analysis single transcript 4.5 kb expressed widely many tissues examined. Unlike the counterpart, levels m exhibited no prominent...
Three mammalian Period ( Per ) genes, termed Per1 , Per2 and Per3 have been identified as structural homologues of the Drosophila circadian clock gene, period per ). The three genes are rhythmically expressed in suprachiasmatic nucleus (SCN), central pacemaker mammals. phases peak mRNA levels for SCN slightly different. Light sequentially induces transcripts but not mice. These data others suggest that each gene has a different partially redundant function To elucidate system vivo we...
This study was undertaken to understand further the mechanisms and dynamics of oxidation lipids in low density lipoprotein (LDL) particles, aiming specifically at elucidating material balance between oxygen uptake products found also relative susceptibilities cholesteryl ester core phosphatidylcholine outer monolayer LDL particles. It that considerable amount could not be accounted for by conjugated diene or total peroxides. Total peroxide measured from phosphine oxide formed...
Abstract Investigations into mechanisms in various cortical areas can be greatly improved and supported by stable recording of single neuronal activity. In this study, fine silicon wire electrodes (diameter 3 μm, length 160 μm) are fabricated vapor–liquid–solid (VLS) growth with the aim stabilizing reducing invasiveness on measurement procedure. The electrode is a modular 1 × mm 2 conductive block that assembled number different device packages, for example rigid or flexible printed circuit...
Microscale needle technology is important in electrophysiological studies, drug/chemical delivery systems, optogenetic applications, and so on. In this study, dissolvable needle-base scaffold realizes penetration of high-aspect-ratio flexible microneedles (e.g., <5 μm diameter >500 length) into biological tissues. This methodology, which applicable to numerous microneedles, should reduce the invasiveness provide safer tissue penetrations than conventional approaches. As a service our authors...
Intracellular recording nanoscale electrode devices provide the advantages of a high spatial resolution and sensitivity. However, length nanowire/nanotube-based nanoelectrodes is currently limited to <10 μm long due fabrication issues for high-aspect-ratio nanoelectrodes. The concept reported here can address technological limitations by fabricating >100 nanoscale-tipped electrodes, which show intracellular capability.