A5050868157- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- Axon Guidance and Neuronal Signaling
- Mesenchymal stem cell research
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Osteoarthritis Treatment and Mechanisms
- Pancreatic and Hepatic Oncology Research
- Bone Metabolism and Diseases
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related gene regulation
- HER2/EGFR in Cancer Research
- Renal and related cancers
- Neuroblastoma Research and Treatments
- Circadian rhythm and melatonin
- Hippo pathway signaling and YAP/TAZ
- Urinary Tract Infections Management
- Cancer Cells and Metastasis
- Radiopharmaceutical Chemistry and Applications
- Glioma Diagnosis and Treatment
- T-cell and B-cell Immunology
Okayama University
1990-2025
Centre National de la Recherche Scientifique
2005-2025
Niigata University of Pharmacy and Medical and Life Sciences
2016-2025
Kyoto Prefectural University of Medicine
2025
Université Paris Cité
2025
RIKEN Center for Integrative Medical Sciences
2010-2019
The University of Tokyo
2010-2018
Mito Saiseikai General Hospital
2018
Ebara (Japan)
2018
Obayashi (Japan)
2018
In vertebrates, densely methylated DNA is associated with inactive transcription.Actors in this process include proteins of the MBD family that can recognize CpGs and repress transcription.Kaiso, a structurally unrelated protein, has also been shown to bind CGCGs through its three Kru ¨ppel-like C 2 H zinc fingers.The human genome contains two uncharacterized proteins, ZBTB4 ZBTB38, contain Kaiso-like fingers.We report ZBTB38 vitro vivo.Unlike Kaiso, they single CpGs.When transfected mouse...
Circadian rhythm disturbances are well established in neurological diseases. However, how these disruptions cause homeostatic imbalances remains poorly understood. Brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein 1 (Bmal1) is a major circadian clock transcriptional activator, Bmal1 deficiency male Bmal1nestin-/- mice induced marked astroglial activation without affecting the number of astrocytes brain spinal cord. deletion caused blood-brain barrier (BBB)...
Although adoptive transfer of cytotoxic T lymphocytes (CTL) offer a promising cancer therapeutic direction, the generation antigen-specific CTL from patients has faced difficulty in efficient expansion ex vivo culture. To resolve this issue, several groups have proposed that induced pluripotent stem cell technology be applied for CTL, which retain expression same T-cell receptor as original CTL. However, these previous studies, regenerated are mostly CD8αα+ innate type and less activity than...
ABSTRACT We have previously shown that endochondral ossification is finely regulated by the Clock system expressed in chondrocytes during postnatal skeletogenesis. Here we show a sophisticated modulation of bone resorption and mass through its expression bone-forming osteoblasts. Brain muscle aryl hydrocarbon receptor nuclear translocator-like protein 1 (Bmal1) Period1 (Per1) were with oscillatory rhythmicity vivo, circadian rhythm was also observed cultured osteoblasts Per1::luciferase...
Abstract T‐cell acute leukemia and lymphoma have a poor prognosis. Although new therapeutic agents been developed, their effects are suboptimal. α‐Pinene, monoterpene compound, has an antitumor effect on solid tumors; however, few comprehensive investigations conducted its impact hematologic malignancies. This report provides analysis of the potential benefits using α‐pinene as agent for treatment tumors. We found that inhibited proliferation malignancies, especially in tumor cell lines EL‐4...
Polycomb group (PcG) proteins play an important role in the control of developmental gene expression higher organisms. In mammalian systems, PcG participate pluripotency, cell fate, cycle regulation, X chromosome inactivation and parental imprinting. this study we have analysed function mouse protein polycomblike 2 (Pcl2), one three homologues Drosophila Polycomblike (Pcl) protein. We show that Pcl2 is expressed at high levels during early embryogenesis embryonic stem (ES) cells. At...
Reprogramming of antigen-specific T lymphocytes into induced pluripotent stem cells (iPSCs) and their subsequent re-differentiation has enabled expansion functional in vitro, thus opening up new approaches for immunotherapy cancer other diseases. In this study, we have established a robust protocol to reprogram human invariant NKT (Vα24+ iNKT) cells, which been shown act as cellular adjuvants exert anti-tumor activity mice humans, re-differentiate the iNKT cell-derived iPSCs cells. These...
Objective: To identify factors predicting the development of high-risk lesions in remnant pancreas after surgery for intraductal papillary mucinous neoplasm (IPMN). Background: IPMN has unique features, including multifocality, adenoma-carcinoma sequence, and distinct pancreatic ductal adenocarcinoma (PDAC) same pancreas. Careful attention should, therefore, be paid to metachronous occurrence lesions, high-grade dysplasia or invasive carcinoma (HGD/INV) concomitant PDAC partial...
Similar to neurons, microglia have an intrinsic molecular clock. The master clock oscillator Bmal1 modulates interleukin‐6 upregulation in microglial cells exposed lipopolysaccharide. can play a role inflammatory responses. We previously demonstrated that gliotransmitter ATP induces transient expression of the gene Period1 via P2X7 purinergic receptors cultured microglia. In this study, we further investigated mechanisms underlying regulation pro‐inflammatory cytokine production by molecules...
The Polycomb group of proteins forms at least two distinct complexes designated the repressive complex-1 (PRC1) and PRC2. These cooperate to mediate transcriptional repression their target genes, including Hox gene cluster Cdkn2a genes. Mammalian Polycomb-like Pcl2/Mtf2 is expressed as four different isoforms, longest one contains a Tudor domain plant homeodomain (PHD) fingers. Pcl2 complex with PRC2 binds genes in PRC2-dependent manner. We show that functional component required for...
The immune system encompasses acquired and innate immunity that matures through interaction with microenvironmental components. Cytokines serve as environmental factors foster functional maturation of cells. Although NOD/SCID/IL2rgKO (NSG) humanized mice support investigation human in vivo, a species barrier between cells the mouse microenvironment limits well function. To study roles cytokines cell development, we created NSG expressing hIL-7 hIL-15. alone was not sufficient for supporting...
Despite recent advances in pluripotent stem cell-based approaches to induce skeletal cells, recapitulating human limb development terms of structure and longitudinally oriented growth remains an unresolved challenge. Here, we report a method differentiate cells into region-specific organoids harboring GDF5+PRG4+ interzone/articular chondrocyte progenitors (IZ/ACPs) SP7+ plate chondrocytes (GPCs) via PRRX1⁺ limb-bud mesenchymal cells. Comparative analysis demonstrated marked similarities...
NKT cells demonstrate antitumor activity when activated to produce Th1 cytokines by DCs loaded with alpha-galactosylceramide, the prototypic cell-activating glycolipid antigen. However, most patients do not have sufficient numbers of induce an effective immune response in this context, indicating a need for source that could be used supplement endogenous cell population. Induced pluripotent stem (iPSCs) hold tremendous potential cell-replacement therapy, but whether it is possible generate...
Induced pluripotent stem cell (iPSC)-derived T cells may provide future therapies for cancer patients, but those generated by current methods, such as the OP9/DLL1 system, have shown abnormalities that pose major barriers clinical translation. Our data indicate these iPSC-derived CD8 single-positive are more like CD4
Pancreatic juice is a promising type of diagnostic sample for pancreatic cancer, and members the mucin (MUC) family are candidates. To evaluate utility MUC as markers, we measured mRNA expression in tissues obtained from patients with different diseases well cancer cell lines by real-time PCR. Furthermore, to support possibility early diagnosis quantification MUC1 MUC5AC, immunohistochemistry microdissection-based quantitative analysis were carried out. There was no significant correlation...