A5066928395- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Cancer-related gene regulation
- Cellular transport and secretion
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- CAR-T cell therapy research
- Chromatin Remodeling and Cancer
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Extracellular vesicles in disease
- Neuroscience and Neuropharmacology Research
- Tissue Engineering and Regenerative Medicine
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Acute Myeloid Leukemia Research
- Pluripotent Stem Cells Research
- Immunotherapy and Immune Responses
- Histone Deacetylase Inhibitors Research
- Glycosylation and Glycoproteins Research
Erasmus MC
2016-2025
Erasmus University Rotterdam
2015-2024
Istituti di Ricovero e Cura a Carattere Scientifico
2023
Agostino Gemelli University Polyclinic
2023
Università Cattolica del Sacro Cuore
2023
ORCID
2020
Codarts Rotterdam
2019
Netherlands Metabolomics Centre
2014-2017
Maastricht University
2016
Shenzhen University
2015
Polycomb-repressive complex 1 (PRC1) has a central role in the regulation of heritable gene silencing during differentiation and development. PRC1 recruitment is generally attributed to interaction chromodomain core protein Polycomb with trimethyl histone H3K27 (H3K27me3), catalyzed by second complex, PRC2. Unexpectedly we find that RING1B, catalytic subunit PRC1, associated monoubiquitylation H2A are targeted closely overlapping sites wild-type PRC2-deficient mouse embryonic stem cells...
Transcription factors, such as Oct4, are critical for establishing and maintaining pluripotent cell identity. Whereas the genomic locations of several pluripotency transcription factors have been reported, spectrum their interaction partners is underexplored. Here, we use an improved affinity protocol to purify Oct4-interacting proteins from mouse embryonic stem cells (ESCs). Subsequent purification Oct4 Sall4, Tcfcp2l1, Dax1, Esrrb resulted in interactome 166 proteins, including...
Recent evidence indicates there is a role for small membrane vesicles, including exosomes, as vehicles intercellular communication. Exosomes secreted by most cell types can mediate transfer of proteins, mRNAs, and microRNAs, but their in the transmission infectious agents less established. studies have shown that hepatocyte-derived exosomes containing hepatitis C virus (HCV) RNA activate innate immune cells, HCV between hepatocytes remains unknown. In this study, we investigated whether...
DNA double-strand break (DSB) repair by nonhomologous end joining (NHEJ) requires the assembly of several proteins on ends. Although biochemical studies have elucidated aspects NHEJ reaction mechanism, much less is known about in living cells, mainly because inability to visualize at damage. Here we provide evidence that a pulsed near IR laser can produce DSBs without any visible alterations nucleus, and show accumulate irradiated areas. The levels Ku accumulation diminished time, showing...
BICD2 is one of the two mammalian homologues Drosophila Bicaudal D, an evolutionarily conserved adaptor between microtubule motors and their cargo that was previously shown to link vesicles mRNP complexes dynein motor. Here, we identified a G2-specific role for in relative positioning nucleus centrosomes dividing cells. By combining mass spectrometry, biochemical cell biological approaches, show nuclear pore complex (NPC) component RanBP2 directly binds recruits it NPCs specifically G2 phase...
Cytoplasmic dynein is the major microtubule minus-end–directed cellular motor. Most activities require dynactin, but mechanisms regulating cargo-dependent dynein–dynactin interaction are poorly understood. In this study, we focus on recruitment to cargo by conserved motor adaptor Bicaudal D2 (BICD2). We show that and dynactin depend each other for BICD2-mediated targeting BICD2 N-terminus (BICD2-N) strongly promotes stable between both in vitro vivo. Direct visualization of live cells...
Sending neural stem cells back to the garage In brain's hippocampus, which modulates memories and emotions, generate new neurons, even during adulthood. How many neurons are generated, when, follows from balance between quiescence proliferation in pool of cells. Urbán et al. asked what signals send proliferating into a quiescent state. They found that key transcription factor promotes cellular was degraded through ubiquitinylation system. This molecular interaction regulated return resting...
Article11 January 2021Open Access Transparent process An organoid-derived bronchioalveolar model for SARS-CoV-2 infection of human alveolar type II-like cells Mart M Lamers orcid.org/0000-0002-1431-4022 Viroscience Department, Erasmus University Medical Center, Rotterdam, The NetherlandsThese authors contributed equally to this work Search more papers by author Jelte van der Vaart orcid.org/0000-0002-4126-8420 Oncode Institute, Hubrecht Royal Netherlands Academy Arts and Sciences Utrecht,...
DNA-protein crosslinks (DPCs) arise from enzymatic intermediates, metabolism or chemicals like chemotherapeutics. DPCs are highly cytotoxic as they impede DNA-based processes such replication, which is counteracted through proteolysis-mediated DPC removal by spartan (SPRTN) the proteasome. However, whether affect transcription and how transcription-blocking repaired remains largely unknown. Here we show that severely RNA polymerase II-mediated preferentially in active genes...
Membrane model systems consisting of phosphatidylcholines and hydrophobic α-helical peptides with tryptophan flanking residues, a characteristic motif for transmembrane protein segments, were used to investigate the contribution tryptophans peptide−lipid interactions. Peptides different lengths at positions in sequence incorporated relatively thick or thin lipid bilayers. The organization was assessed by NMR methods hydrogen/deuterium exchange combination mass spectrometry. Previously, it...
Transcription regulation involves enzyme-mediated changes in chromatin structure. Here, we describe a novel mode of histone crosstalk during gene silencing, which H2A monoubiquitylation is coupled to the removal H3 Lys 36 dimethylation (H3K36me2). This pathway was uncovered through identification dRING-associated factors (dRAF), Polycomb group (PcG) silencing complex harboring ubiquitin ligase dRING, PSC and F-box protein, demethylase dKDM2. In vivo, dKDM2 shares many transcriptional targets...
The vesicle-associated membrane protein-associated proteins (VAPs) VAPA and VAPB interact with lipid-binding carrying a short motif containing two phenylalanines in an acidic tract (FFAT motif) targets them to the cytosolic surface of endoplasmic reticulum (ER). A genetic mutation (P56S) conserved major sperm protein homology domain has been linked motor-neuron degeneration affected amyotrophic lateral sclerosis (ALS) patients. We report that CNS, is abundant motor neurons P56S substitution...
Ring1B/Rnf2 is a RING finger protein member of the Polycomb group (PcG) proteins, which form chromatin-modifying complexes essential for embryonic development and stem cell renewal are commonly deregulated in cancer. ubiquitin E3 ligase that catalyzes monoubiquitylation histone H2A, one modifications needed transcriptional repression activity PcG proteins. was shown to be part two complexes, PRC1 complex E2F6.com-1 complex, also contains non-PcG members, thus raising prospect additional...