A5065375123- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Carcinogens and Genotoxicity Assessment
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Advanced biosensing and bioanalysis techniques
- PARP inhibition in cancer therapy
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Fluorescence Microscopy Techniques
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Chromosomal and Genetic Variations
- bioluminescence and chemiluminescence research
- Gastrointestinal motility and disorders
- RNA and protein synthesis mechanisms
- Telomeres, Telomerase, and Senescence
- Chromatin Remodeling and Cancer
- Photosynthetic Processes and Mechanisms
- Liver Disease Diagnosis and Treatment
- Enzyme Structure and Function
Erasmus MC
2016-2025
Erasmus MC Cancer Institute
2021-2025
Oncode Institute
2017-2024
Seqirus (United States)
2021
Erasmus University Rotterdam
2007-2019
Cancer Genomics Centre
2006-2018
University of Antwerp
2009-2014
Institut de Radiobiologie Cellulaire et Moléculaire
2013
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2013
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013
The human BTF2 basic transcription factor (also called TFIIH), which is similar to the δ in rat and b yeast, required for class II gene transcription. A strand displacement assay was used show that highly purified preparation of had an adenosine triphosphate-dependent DNA helicase activity, addition previously characterized carboxyl-terminal domain kinase activity. Amino acid sequence analysis tryptic digest generated from 89-kilodalton subunit indicated this polypeptide corresponded ERCC-3...
The DNA polymerase processivity factor proliferating cell nuclear antigen (PCNA) is central to both replication and repair. ring-shaped homotrimeric PCNA encircles slides along double-stranded DNA, acting as a "sliding clamp" that localizes proteins DNA. We determined the behavior of green fluorescent protein-tagged human (GFP-hPCNA) in living cells analyze its different engagements Photobleaching tracking foci revealed dynamic equilibrium between two kinetic pools PCNA, i.e., bound free...
The WD40-repeat protein DDB2 is essential for efficient recognition and subsequent removal of ultraviolet (UV)-induced DNA lesions by nucleotide excision repair (NER). However, how promotes NER in chromatin poorly understood. Here, we identify poly(ADP-ribose) polymerase 1 (PARP1) as a novel DDB2-associated factor. We demonstrate that facilitated poly(ADP-ribosyl)ation UV-damaged through the activity PARP1, resulting recruitment chromatin-remodeling enzyme ALC1. Depletion ALC1 rendered cells...
DNA-protein crosslinks (DPCs) arise from enzymatic intermediates, metabolism or chemicals like chemotherapeutics. DPCs are highly cytotoxic as they impede DNA-based processes such replication, which is counteracted through proteolysis-mediated DPC removal by spartan (SPRTN) the proteasome. However, whether affect transcription and how transcription-blocking repaired remains largely unknown. Here we show that severely RNA polymerase II-mediated preferentially in active genes...
The Cockayne syndrome B protein (CSB) is required for coupling DNA excision repair to transcription in a process known as transcription-coupled (TCR). patients show UV sensitivity and severe neurodevelopmental abnormalities. CSB DNA-dependent ATPase of the SWI2/SNF2 family. SWI2/SNF2-like proteins are implicated chromatin remodeling during transcription. Since structure also affects efficiency, activities within expected. Here we used purified recombinant investigate whether it can remodel...
To study the nuclear organization and dynamics of nucleotide excision repair (NER), endonuclease ERCC1/XPF (for cross complementation group 1/xeroderma pigmentosum F) was tagged with green fluorescent protein its mobility monitored in living Chinese hamster ovary cells. In absence DNA damage, complex moved freely through nucleus, a diffusion coefficient (15 ± 5 square micrometers per second) consistent molecular size. Ultraviolet light–induced damage caused transient dose-dependent...
The molecular pathway of p53-dependent apoptosis (programmed cell death) is poorly understood. Because p53 binds to the basal transcription-repair complex TFIIH and modulates its DNA helicase activities, we hypothesized that helicases XPB XPD are members p53-mediated apoptotic pathway. Whereas transfer a wild-type expression vector by microinjection or retroviral infection into primary normal human fibroblasts resulted in apoptosis, from individuals with xeroderma pigmentosum (XP), who...
Heterochromatin protein 1 (HP1) family members are chromatin-associated proteins involved in transcription, replication, and chromatin organization. We show that HP1 isoforms HP1-α, HP1-β, HP1-γ recruited to ultraviolet (UV)-induced DNA damage double-strand breaks (DSBs) human cells. This response requires the chromo shadow domain of is independent H3K9 trimethylation detect UV DSBs. Loss results high sensitivity light ionizing radiation nematode Caenorhabditis elegans, indicating essential...