A5044605627- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neuropharmacology Research
- Genomics and Chromatin Dynamics
- Herbal Medicine Research Studies
- Chromosomal and Genetic Variations
- Ion channel regulation and function
- Sperm and Testicular Function
- Ovarian function and disorders
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Ion Channels and Receptors
- Bone Tissue Engineering Materials
- Neurogenesis and neuroplasticity mechanisms
- Electrospun Nanofibers in Biomedical Applications
Merck (Germany)
2022
Instituto Gulbenkian de Ciência
2011-2018
Erasmus MC
2008-2012
Universidade do Porto
2009
Erasmus University Rotterdam
2009
End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles three mammalian EBs in controlling dynamics and analyzed domains involved. Protein depletion rescue experiments showed that EB1 EB3, but not EB2, promote persistent growth by suppressing catastrophes. Furthermore, demonstrated vitro cells EB behavior depends on calponin homology domain does require dimer formation. In contrast, dimerization is...
The ends of growing microtubules (MTs) accumulate a set diverse factors known as MT plus end–tracking proteins (+TIPs), which control microtubule dynamics and organization. In this paper, we identify SLAIN2 key component +TIP interaction networks. We showed that the C-terminal part bound to end-binding (EBs), cytoplasmic linker (CLIPs), CLIP-associated characterized in detail with EB1 CLIP-170. Furthermore, found N-terminal interacted ch-TOG, mammalian homologue polymerase XMAP215. Through...
Dynamic microtubules are important to maintain neuronal morphology and function, but whether activity affects the organization of dynamic is unknown. Here, we show that a protocol induce NMDA-dependent long-term depression (LTD) rapidly attenuates microtubule dynamics in primary rat hippocampal neurons, removing microtubule-binding protein EB3 from growing plus-ends dendrites. This effect requires entry calcium mediated by activation NR2B-containing NMDA-type glutamate receptor. The rapid...
ABSTRACT The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to existing structure (canonical duplication) or de novo. How centrosomes novo not known. master driver biogenesis, PLK4, critical for recruitment several centriole components. Here, we investigate beginning taking advantage Xenopus egg...
Here we demonstrate an efficient method to fabricate large-domain monodisperse foam scaffolds made of gelatin for 3D cell culture. We tested three distinct tissue types cultured in composed uniform spherical pores. The cells displayed appropriate morphological and physiological characteristics: epithelial formed cyst-like structures were polarized inside pores, myoblasts adopted a tubular structure fused into myotubes, fibroblasts exhibited wide variety morphologies. Scaffolds with pores can...
Establishment and maintenance of the mitotic spindle requires balanced activity microtubule-associated proteins motors. In this study we have addressed how microtubule plus-end tracking protein Mast/Orbit/CLASP cytoplasmic dynein regulate process in Drosophila melanogaster embryos S2 cells. We show that Mast accumulates at kinetochores early mitosis, which is followed by a poleward streaming upon attachment. This leads to reduction levels during metaphase anaphase depends largely on minus...
Summary statement PLK4 binds to microtubules and self assembles into supramolecular assemblies that recruit tubulin trigger de novo MTOC formation in Xenopus laevis extracts. Abstract The centrosome is an important microtubule-organizing center (MTOCs) animal cells it consists of two barrel-shaped structures (centrioles), surrounded by the pericentriolar material (PCM), which nucleates microtubules. PCM components form condensates, concentrate microtubule nucleators. Centrosomes can close...