Development of miniaturized devices that enable rapid and direct analysis the specific binding small molecules to proteins could be substantial importance discovery screening for new drug molecules. Here, we report highly sensitive label-free electrical detection small-molecule inhibitors ATP Abl by using silicon nanowire field-effect transistor devices. Abl, which is a protein tyrosine kinase whose constitutive activity responsible chronic myelogenous leukemia, was covalently linked...

The merger of nanoscale building blocks with flexible and/or low cost substrates could enable the development high-performance electronic and photonic devices potential to impact a broad spectrum applications. Here we demonstrate that high-quality, single-crystal nanowires can be assembled onto inexpensive glass plastic create basic transistor light-emitting diode devices. In our approach, high-temperature synthesis is separated from ambient-temperature solution-based assembly fabrication...

We investigate the self-assembly of colloidal spheres on periodically patterned templates. The surface potentials and phases are induced entropically by presence dissolved, nonadsorbing polymers. A rich variety two-dimensional fluidlike solidlike was observed to form template with both one- symmetry. same methodology then used nucleate an oriented single fcc crystal more than 30 layers thick. general approach provides a new route for directed novel mesoscopic structures.

In this paper, we demonstrate for the first time technique to using microfluidics fabricate tissue engineering scaffolds with uniform pore sizes. We investigate both bubble generation of microfluidic device and application foam as a scaffold. Our consists two concentric tapered channels, which are made by micropipettes. Nitrogen gas aqueous alginate solution Pluronic® F127 surfactant pumped through inner outer respectively. observe rich dynamic patterns bubbles encapsulated in liquid...

In this paper we present fundamental studies elucidating the role of entropy in particle suspensions. We focus on systems composed large colloidal particles along with a second, usually smaller species such as or polymer. describe direct measurements these interactions suspension, and systematically show how forces can be used to control self–assembly particles. The provides unified review experiments from our laboratory, few cases touches very recent results.

We report direct measurements of entropic interactions colloidal spheres in suspensions rodlike fd bacteriophage. investigate the influence sphere size, rod concentration, and ionic strength on these interactions. Although results compare favorably with a recent calculation, small discrepancies reveal effects due to flexibility. At high salt concentrations, potential turns repulsive as result viral adsorption bridging between spheres.

A new type of polymer-assisted self-assembly nanospheres at a water-air interface was uncovered. By adding merely 1-3 ppm polyethylene oxide in the water, polystyrene nanospheres, applicable to diameters ranging from 100 nm 1 μm, were found gradually move closer each other and eventually form close-packed structure confirmed its diffraction pattern. As it turns out, oxides are adsorbed onto surface giving rise effective screening coulomb repulsive force between followed by onset...

In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells foam scaffolds which resemble vivo pulmonary alveoli may give more insight on cellular behaviors vivo. consists ordered arrays uniform spherical pores gelatin. We found that cell morphology was different from 2D substrate. Next, applied direct current electric field (EF) 338 mV/mm through for study cells' migration within....

Abstract The spatial boundary condition (SBC) arising from the surrounding microenvironment imposes specific geometry and constraints that affect organogenesis tissue homeostasis. Mesenchymal stromal cells (MSCs) sensitively respond to alterations of mechanical cues generated SBC. However, provided by a three-dimensional (3D) environment are deprived in reductionist 2D culture system. This study investigates how SBC affects osteogenic differentiation MSCs using 3D scaffolds with...

We explore magnetic-field-induced ordering and microphase separation of aqueous ferrofluid mixtures with nonmagnetic latex spheres. The is a surfactant stabilized suspension magnetite (Fe3O4) particles average diameter 20 nm (including the approximately 2.5-nm thick layer); spheres are charge polymethylmethacrylate (PMMA) diameters 42 nm, 108 220 nm. In presence uniform magnetic field, needlelike droplets formed that eventually grew to sample-traversing columns at fields 600 G;...

Here we demonstrate an efficient method to fabricate large-domain monodisperse foam scaffolds made of gelatin for 3D cell culture. We tested three distinct tissue types cultured in composed uniform spherical pores. The cells displayed appropriate morphological and physiological characteristics: epithelial formed cyst-like structures were polarized inside pores, myoblasts adopted a tubular structure fused into myotubes, fibroblasts exhibited wide variety morphologies. Scaffolds with pores can...

We developed a microfluidic device to culture cellular spheroids of controlled sizes and suitable for live cell imaging by selective plane illumination microscopy (SPIM). cocultured human umbilical vein endothelial cells (HUVECs) within the formed hepatocellular carcinoma cells, studied distributions HUVECs over time. observed that migration depended on size spheroids. In ∼200 μm diameters, migrated outwards edges 48 h; while in ∼250 there was no outward up 72 h. addition, we effects...

Journal Article Three-dimensional fibroblast morphology on compliant substrates of controlled negative curvature Get access Yi-hsuan Lee, Lee Institute Physics, Academia Sinica, Taipei, Taiwan 11529. Fax: +886-22788-9829; Tel: +886-22789-6763 E-mail: leey@phys.sinica.edu.tw Search for other works by this author on: Oxford Academic Google Scholar Jung-ren Huang, Huang Department National Normal University, 11677 jrhuang@ntnu.edu.tw Yang-kao Wang, Wang Graduate Biomedical Materials and Tissue...

Atomic force microscopy (AFM) is becoming an increasingly popular method for studying cell mechanics, however the existing analysis tools determining elastic modulus from indentation experiments are unable to quantitatively account mechanical heterogeneity commonly found in biological samples. In this work, we numerically calculated force-indentation curves onto two-layered materials using analytic model. We that effect of underlying substrate can be predicted by mismatch moduli and...

In vivo, epithelial cells maintain structural integrity under dynamic mechanical perturbations. To study this, we treated various cell lines with long-term cyclic stretch (CS). Surprisingly, transitioned from cuboidal to columnar shape (columnarization) in MDCK cells, while others only elongated. This change correlated actin accumulation at the top and stress fiber realignment bottom. Blocking stimulation via FAK inhibition or reducing vinculin partially prevented columnarization; however,...

We report optical switching studies on nematic liquid crystal incorporated into structures based self-assembled colloids. compare the electro-optic responses of imbibed colloid-templated polymers, in interstitial space colloid crystals, and conventional polymer-dispersed crystals. characterize Bragg diffraction our templated liquid-crystal/polymer composites as a function electric field measure times. The response connected networks differs qualitatively from that isolated cavities.

We demonstrate that square two-dimensional grating templates can drive the growth of three-dimensional, face-centered-cubic (fcc) colloidal crystals by convective assembly. The symmetry [i.e., (100) planes parallel to substrate] underlying template was transferred crystal and maintained throughout its ∼50 layers. characterized grown on flat templated substrates using electron microscopy small-angle x-ray scattering (SAXS). SAXS measurements samples clearly show fourfold diffraction patterns...

ABSTRACT A tissue engineering chondrocytes/scaffold construct provides a promise to cartilage regeneration. The architecture of scaffold such as interconnections, porosities, and pore sizes influences the fates seeding cells including gene expression, survival, migration, proliferation, differentiation thus may determine success this approach. Scaffolds highly ordered uniform structures are desirable control cellular behaviors. In study, newly designed microfluidic device based on...