A5053920984- Cellular Mechanics and Interactions
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Magnesium in Health and Disease
- Electron Spin Resonance Studies
- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Wnt/β-catenin signaling in development and cancer
- Ion channel regulation and function
- Blood Pressure and Hypertension Studies
- Liver physiology and pathology
- Hemodynamic Monitoring and Therapy
- Bone and Dental Protein Studies
- Renal and related cancers
- Dialysis and Renal Disease Management
- Cardiac electrophysiology and arrhythmias
- TGF-β signaling in diseases
- Chronic Kidney Disease and Diabetes
- Tendon Structure and Treatment
- Mass Spectrometry Techniques and Applications
- Tissue Engineering and Regenerative Medicine
- Bone Metabolism and Diseases
- Advanced NMR Techniques and Applications
- Ion Channels and Receptors
- Magnesium Alloys: Properties and Applications
National Tsing Hua University
1997-2023
Chang Gung University
2013-2022
National Yang Ming Chiao Tung University
2014-2021
Chang Gung Memorial Hospital
2020-2021
Memorial Hospital of South Bend
2021
National Yang Ming University Hospital
2016
Yale University
2015
University of Virginia
2001-2014
National Taiwan Normal University
2012
Chung Hwa University of Medical Technology
2008
There is a growing interest in cell therapies using mesenchymal stromal cells (MSCs) for repairing bone defects. MSCs have the ability to differentiate into osteoprogenitors and osteoblasts as well form calcified matrix. However, molecular mechanisms governing mineralization during osteogenic differentiation remain unclear. Non-collagenous proteins extracellular matrix are believed control different aspects of mineralization. Since osteocalcin most abundant non-collagenous protein, purpose...
Abstract Microenvironments that modulate fate commitments of mesenchymal stromal cells (MSCs) are composed chemical and physical cues, but the latter ones much less investigated. Here we demonstrate intermittent fluid shear stress (IFSS), a potent physiologically relevant mechanical stimulus, regulates osteogenic differentiation MSCs through Transient receptor potential melastatin 7 (TRPM7)-Osterix axis. Immunostaining showed localization TRPM7 near or at cell membrane upon IFSS calcium...
Current SDSL-EPR methods allow measurement of dipolar distances in the 8-70 A range; however, use extrinsic probes complicates interpretation these modeling macromolecular structure and conformational changes. The data presented here show that interprobe correlate only weakly with Cbeta-Cbeta distances, especially for are on order spin label tether lengths. Explicitly incorporating into process increases experiment/model correlation 4-fold reduces distance error from 6 to 3 A.
Addition of menthol to cigarettes may be associated with increased initiation smoking. The potential mechanisms underlying this association are not known. Menthol, likely due its effects on cold-sensing peripheral sensory neurons, is known inhibit the sensation irritation elicited by respiratory irritants. However, it remains unclear whether modulates cigarette smoke irritancy and nicotine absorption during initial exposures cigarettes, thereby facilitating smoking initiation. Using...
Abstract The spatial boundary condition (SBC) arising from the surrounding microenvironment imposes specific geometry and constraints that affect organogenesis tissue homeostasis. Mesenchymal stromal cells (MSCs) sensitively respond to alterations of mechanical cues generated SBC. However, provided by a three-dimensional (3D) environment are deprived in reductionist 2D culture system. This study investigates how SBC affects osteogenic differentiation MSCs using 3D scaffolds with...
TGF-beta is a therapeutic target for renal fibrosis. Scientists have long sought ways to antagonize ameliorate diabetic nephropathy. Bone morphogenetic protein (BMP-2) member of the superfamily and highly regulated in kidney. Thus, role BMP-2 was investigated NRK-49F cells (rat fibroblasts). We showed that TGF-beta1 induces an increase fibronectin. Treatment with exogenous or pCMV-BMP-2 significantly reversed TGF-beta1-induced fibronectin concomitant significant decrease type I receptors...
Cardiotoxins (CTXs) from cobra venom show cytotoxicity toward several cell types. They cause systolic heart arrest and severe tissue necrosis. Their interaction with phospholipids is established but by itself fails to explain the specificity of these toxins; other component(s) membrane must, therefore, intervene direct them their target. We herein show, for first time, that sulfated glycosaminoglycans, heparin, heparan sulfate (HS), chondroitin (CS), dermatan (DS), interact CTX A3, a major...
Magnesium is essential for numerous physiological functions. exists mostly in bone and the amount dynamically regulated by skeletal remodeling. Accelerating mass loss occurs when magnesium intake insufficient; whereas high could lead to mineralization defects. However, underlying regulatory mechanisms remain elusive. In present study, we investigated effects of extracellular concentration on osteogenic differentiation mesenchymal stromal/stem cells (MSCs) role transporter SLC41A1 process....
Abstract Lineage commitment and differentiation of mesenchymal stromal cells (MSCs) into osteoblasts in vitro is enhanced by a potent synthetic form glucocorticoid (GC), dexamethasone (Dex). Paradoxically, when used chronically patients, GCs exert negative effects on bone, phenomenon known as glucocorticoid-induced osteoporosis clinical practice. The mechanism how GC differentially affects bone precursor to become mature during osteogenesis remains elusive. In this study, the dose temporal...
Abstract Cellular biophysical properties are novel biomarkers of cell phenotypes which may reflect the status differentiating stem cells. Accurate characterizations cellular properties, in conjunction with corresponding biochemical could help to distinguish cells from primary cells, cancer and differentiated However, correlated evolution these course directed differentiation has not been well characterized. In this study, we applied video particle tracking microrheology (VPTM) measure...
Human mesenchymal stem cells (hMSCs) can differentiate into osteoblasts and are regulated by chemical cues. The recombinant N-terminal (1-34 amino acids) fragment of the parathyroid hormone (PTH (1-34)) is identified to promote osteogenesis. osteoanabolic effects intermittent PTH (1-34) treatment linked a complex consisting signaling pathways; additionally, protein kinase C (PKC) act as mediators multifunctional transduction pathways, but role PKC δ (PKCδ), downstream target in regulating...
Abstract Background Mesenchymal stromal cells (MSCs) are multipotent and have great potential in cell therapy. Previously we reported the differentiation of human MSCs into hepatocytes vitro that these can rescue fulminant hepatic failure. However, conventional static culture method neither maintains growth factors at an optimal level constantly nor removes cellular waste efficiently. In addition, not only is duration differentiating hepatocyte lineage from required to improve, but also need...
Hemodialysis (HD) therapy is an indispensable tool used in critical care management. Patients undergoing HD are at risk for intradialytic adverse events, ranging from muscle cramps to cardiac arrest. So far, there no effective device-integrated algorithm assist medical staff response these events a step earlier during HD.We aimed develop machine learning algorithms predict unbiased manner.Three-month dialysis and physiological time-series data were collected all patients who underwent...
In vitro differentiation of stem cells into specific cell lineages provides a stable supply for therapy and tissue engineering. Therefore, understanding the mechanisms underlying such processes is critical generating committed lineage-specific progenies effectively. We previously developed two-step protocol to differentiate mesenchymal stromal (MSCs) hepatocyte-like cells. Since hepatic involves mesenchymal-epithelial transition (MET), we hypothesize that promoting MET could further...
Abstract Progressive renal disease is characterized by the accumulation of extracellular matrix proteins in interstitium. Hence, developing agents that antagonize fibrogenic signals a critical issue facing researchers. The present study investigated blood‐circulation‐promoting Chinese herb, safflower, on fibrosis status NRK‐49F cells, normal rat kidney interstitial fibroblast, to evaluate underlying signal transduction mechanism transforming growth factor‐beta (TGF‐β), potent factor....