Abstract Kaempferol is a natural flavonoid. Previous studies have reported that kaempferol has anti‐proliferation activities and induces apoptosis in many cancer cell lines. However, there are no reports on human osteosarcoma. In this study, we investigate the anti‐cancer effects molecular mechanisms of osteosarcoma cells. Our results demonstrate significantly reduces viabilities U‐2 OS, HOB 143B cells, especially OS cells dose‐dependent manner, but exerts low cytotoxicity fetal osteoblast...

We present a novel testing scheme for TSVs in 3D IC by performing on-chip TSV monitoring before bonding, using sense amplification technique that is commonly seen on DRAM. By virtue of the inherent capacitive characteristics, we can detect faulty with little area overhead circuit under test.

Pre-bond test is preferred for a three-dimensional integrated circuit (3D IC), since it reduces stacking yield loss and thus saves cost. In this paper, we present two schemes testing through-silicon vias (TSVs) by performing on-chip screening before wafer thinning bonding. The first scheme blind TSVs, which have one end floating, using charge-sharing technique commonly seen in DRAM. second open-sleeve shorted to the substrate, voltage-dividing ROM. By virtue of inherent capacitive resistive...

Berberine (BBR) is a natural alkaloid with significant antitumor activities against many types of cancer cells. This study investigated the molecular mechanisms by which BBR suppresses growth HER2-overexpressing breast The results show that induces G1-phase cell cycle arrest interfering expression cyclins D1 and E it cellular apoptosis through induction mitochondria/caspase pathway. data also indicate inhibits promotes down-regulating HER2/PI3K/Akt signaling Furthermore, shown combination...

A new type of polymer-assisted self-assembly nanospheres at a water-air interface was uncovered. By adding merely 1-3 ppm polyethylene oxide in the water, polystyrene nanospheres, applicable to diameters ranging from 100 nm 1 μm, were found gradually move closer each other and eventually form close-packed structure confirmed its diffraction pattern. As it turns out, oxides are adsorbed onto surface giving rise effective screening coulomb repulsive force between followed by onset...

Cucurbitacin E, a tetracyclic triterpenes compound extracted from cucurbitaceous plants, has been shown to exhibit anticancer and anti-inflammatory activities. The purpose of this study was elucidate whether cucurbitacin E promotes cell cycle arrest induces apoptosis in T24 cells further explore the underlying molecular mechanisms. effects on cell's growth accompanied morphological changes were examined by MTT assay phase-contrast microscope. DNA content, mitochondrial membrane potential...

Liver cancer is one of the most commonly diagnosed cancers and leading cause death in human populations. Butein, a tetrahydroxychalcone, has been shown to induce apoptosis many cells, but effects butein on migration invasion liver cells are not reported. Herein, we found that effective suppression SK-HEP-1 hepatocarcinoma by using Matrigel cell assay system. The gelatin zymography indicated inhibited activity matrix metalloproteinases 2 (MMP-2) MMP-9. Western blotting analysis decreased...

Chrysophanol (1,8‐dihydroxy‐3‐methylanthraquinone) is one of the anthraquinone compounds, and it has been shown to induce cell death in different types cancer cells. The effects chrysophanol on human lung have not well studied. purpose this study examine chrysophanol‐induced cytotoxic also investigate such influences that involved apoptosis or necrosis A549 cells vitro . Our results indicated decreased viable a dose‐ time‐dependent manner. promoted release reactive oxygen species (ROS) Ca 2+...

To investigate the effects of ellagic acid on growth inhibition TSGH8301 human bladder cancer cells in vitro, were incubated with various doses for different time periods. The phase-contrast microscope was used examining and photographing morphological changes cells. Flow cytometric assay to measure percentage viable cells, cell cycle distribution, apoptotic ROS, mitochondrial membrane potential (ΔΨm), Ca2+, caspase-9 -3 activities after exposure acid. Western blotting examine apoptosis...

Objectives: To focus on bee venom‐induced apoptosis in human bladder cancer TSGH‐8301 cells and to investigate its signaling pathway ascertain whether intracellular calcium iron (Ca 2+ ) is involved this effect. Methods: Bee cytotoxic effects, productions of reactive oxygen species Ca the level mitochondrial membrane potential (ΔΨm) were analyzed by flow cytometry. Apoptosis‐associated proteins examined Western blot analysis confocal laser microscopy. Results: cell morphological changes...

Phenethyl isothiocyanate (PEITC), an effective anticancer and chemopreventive agent, has been reported to inhibit cancer cell growth through cell-cycle arrest induction of apoptotic events in various human cells models. However, whether PEITC inhibits oral squamous carcinoma HSC-3 its underlying mechanisms is still not well elucidated. In the present study, we evaluated inhibitory effects examined PEITC-modulated apoptosis. The contrast-phase flow cytometric assays were used for examining...

Total mortality and sudden cardiac death is highly prevalent in patients with chronic kidney disease (CKD). In CKD patients, the protein-bound uremic retention solute indoxyl sulfate (IS) independently associated cardiovascular disease. However, underlying mechanisms of this association have yet to be elucidated. The relationship between IS electrocardiographic parameters was investigated a prospective observational study among early patients. arrhythmogenic effect evaluated by vitro...

Tanshinone IIA extracted from Danshen, a popular medicinal herb used in traditional Chinese medicine, exhibits cardio-protective effects. However, the mechanism of its cardioprotective effect is not well established. The aims this study were to examine whether tanshinone may alter angiotensin II (Ang II)-induced cell proliferation and identify putative underlying signaling pathways rat cardiac fibroblasts. Cultured fibroblasts pre-treated with stimulated Ang II, endothelin-1 (ET-1)...

Reliable multilevel resistive switching in nanoscale cells is desirable for the wide adoption of random access memory as next-generation nonvolatile memory. We designed NiO-based arrays multilayered NiO/Pt nanowires to explore effects. Nonpolar reproducibly occurs with significantly reduced voltages, narrow voltage distributions and a robust effect. A high resistance ratio (∼105) between high- low-resistance states enables stable multilevels that can be induced easily by series pulsed...

Doxorubicin (Dox) causes the generation of intracellular reactive oxygen species (ROS) and inactivates insulin-like growth factor 1 (IGF1) signaling, leading to cardiomyocyte apoptosis. IGF-binding protein 3 (IGFBP3) is most abundant circulating IGF1 carrier with high affinity, which has been reported mediate ROS-induced Hypoxia-inducible 1α (HIF1A), an upstream IGFBP3 regulated by prolyl hydroxylase domain (PHD) through hydroxylation. In this study, we investigated role IGFBP3, HIF1A, PHD...

This investigation demonstrates the magnetic properties and nanostructures of CoPt3 wire arrays that were fabricated by electrodeposition using a porous alumina template. The X-ray absorption analysis clearly verified occurrence phase transition in nanowires. significantly influences enhances coercivity squareness. nanowires was from random alloy distribution to anisotropically ordered (L12). thermally induced L12 through "cluster-in-cluster" intermediate state via interdiffusion processes...