A5051446500- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Bone Tissue Engineering Materials
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- Nuclear Structure and Function
- Spinal Fractures and Fixation Techniques
- Photonic and Optical Devices
- Nanopore and Nanochannel Transport Studies
- Silk-based biomaterials and applications
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Protein Tyrosine Phosphatases
- Electrospun Nanofibers in Biomedical Applications
- Biopolymer Synthesis and Applications
- Protist diversity and phylogeny
Utrecht University
2012-2025
Wuhan University
2019-2025
Xi’an University of Posts and Telecommunications
2024-2025
Shanghai Jiao Tong University
2013-2024
Henan Normal University
2014-2024
Boston University
2024
Tsinghua University
2016-2024
China University of Petroleum, East China
2024
Ministry of Education
2019-2024
Beijing Tsinghua Chang Gung Hospital
2024
Cytoplasmic dynein is the major microtubule minus-end–directed cellular motor. Most activities require dynactin, but mechanisms regulating cargo-dependent dynein–dynactin interaction are poorly understood. In this study, we focus on recruitment to cargo by conserved motor adaptor Bicaudal D2 (BICD2). We show that and dynactin depend each other for BICD2-mediated targeting BICD2 N-terminus (BICD2-N) strongly promotes stable between both in vitro vivo. Direct visualization of live cells...
Abstract Severe traumatic bleeding may lead to extremely high mortality rates, and early intervention stop plays as a critical role in saving lives. However, rapid hemostasis deep non-compressible trauma using highly water-absorbent hydrogel, combined with strong tissue adhesion bionic procoagulant mechanism, remains challenge. In this study, DNA hydrogel (DNAgel) network composed of natural nucleic acids water absorption, swelling instant is reported, like band-aid physically bleeding. The...
Significance The main stabilizer of the DNA double helix is not base-pair hydrogen bonds but coin-pile stacking base pairs, whose hydrophobic cohesion, requiring abundant water, indirectly makes interior dry so that can exert full recognition power. We report certain semihydrophobic agents depress energy (measurable in single-molecule experiments), leading to transiently occurring holes stack (monitorable via binding threading intercalators). Similar structures observed complexes with RecA...
The microtubule cytoskeleton regulates cell polarity by spatially organizing membrane trafficking and signaling processes. In epithelial cells, microtubules form parallel arrays aligned along the apico-basal axis, recent work has demonstrated that members of CAMSAP/Patronin family control apical tethering minus ends. Here, we show in mammalian intestinal spectraplakin ACF7 (also known as MACF1) specifically binds to CAMSAP3 is required for localization CAMSAP3-decorated Loss but not or its...
Abstract γ-Tubulin ring complex (γ-TuRC) is the major microtubule-nucleating factor. After nucleation, microtubules can be released from γ-TuRC and stabilized by other proteins, such as CAMSAPs, but biochemical cross-talk between minus-end regulation pathways poorly understood. Here we reconstituted this process in vitro using purified components. We found that all CAMSAPs could bind to minus ends of γ-TuRC-attached microtubules. CAMSAP2 CAMSAP3, which decorate stabilize growing not tracking...
Centrioles are microtubule-based organelles required for the formation of centrosomes and cilia. Centriolar microtubules, unlike their cytosolic counterparts, stable grow very slowly, but underlying mechanisms poorly understood. Here, we reconstituted in vitro interplay between proteins that cap distal centriole ends control elongation: CP110, CEP97, CPAP/SAS-4. We found whereas CEP97 does not bind to microtubules directly, CP110 autonomously binds microtubule plus ends, blocks growth,...
Hyperosmotic stress as physiologic dysfunction can reduce the cell volume and then redistribute both protein concentration ionic strength, but its effect on liquid-liquid phase separation (LLPS) is not well understood. Here, we map hyperosmotic-stress-induced nuclear LLPS of amyotrophic lateral sclerosis (ALS)-related proteins (fused in sarcoma [FUS], TAR DNA-binding 43 [TDP-43]). The dynamic reversibility FUS granules are continuable with increase hypertonic stimulation time, those TDP-43...
The increasing availabilities of GPS-enabled devices have given rise to the location-based social networking services (LBSN), in which users can record their travel experiences with GPS trajectories and share these among each other on Web communities. Usually, far denser points than necessary scenarios GPS-trajectory-sharing. Meanwhile, redundant will decrease performance LBSN systems even cause browser crashed. Existing line simplification algorithms only focus maintaining shape information...
Hfq is a bacterial protein that involved in several aspects of nucleic acids metabolism. It has been described as one the nucleoid associated proteins shaping chromosome, although it better known to influence translation and turnover cellular RNAs. Here, we explore role Escherichia coli C-terminal domain compaction double stranded DNA. Various experimental methodologies, including fluorescence microscopy imaging single DNA molecules confined inside nanofluidic channels, atomic force...
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