A5047305204- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- interferon and immune responses
- Microtubule and mitosis dynamics
- Protein Degradation and Inhibitors
- Chromosomal and Genetic Variations
- Protein Structure and Dynamics
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Trypanosoma species research and implications
- Genetics and Neurodevelopmental Disorders
- Biochemical and Molecular Research
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Cloud Computing and Resource Management
- Cellular transport and secretion
Hefei National Center for Physical Sciences at Nanoscale
2008-2025
University of Science and Technology of China
2016-2025
Hubei University of Medicine
2025
Taihe Hospital
2025
Guiyang Medical University
2025
Yunnan Academy of Forestry
2025
Huazhong University of Science and Technology
2025
Tongji Hospital
2025
Affiliated Hospital of Guizhou Medical University
2025
Veterans Health Administration
2024
The polycomb repressive complex 2 (PRC2) is the major methyltransferase for H3K27 methylation, a modification critical maintaining repressed gene expression programs throughout development. It has been previously shown that PRC2 maintains histone methylation patterns during DNA replication in part through its ability to bind H3K27me3. However, mechanism by which recognizes H3K27me3 unclear. Here we show WD40 domain of EED, component, methyllysine histone-binding domain. crystal structures...
Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. A group of germline specific single and multiTudor domain containing proteins (TDRDs) represented drosophila its mammalian orthologs Tdrd1, Tdrd4/RNF17, Tdrd6 play evolutionarily conserved roles in germinal granule/nuage formation germ cell specification differentiation. However, their physiological ligands, the biochemical structural basis for ligand recognition,...
N(6)-Methyladenosine (m(6)A) is the most prevalent internal RNA modification in eukaryotes. ALKBH5 belongs to AlkB family of dioxygenases and has been shown specifically demethylate m(6)A single-stranded RNA. Here we report crystal structures presence either its cofactors or inhibitor citrate. Catalytic assays demonstrate that catalytic domain can both DNA. We identify TCA cycle intermediate citrate as a modest ALKHB5 (IC50, ∼488 μm). The structural analysis reveals loop region immobilized...
Background The PWWP domain was first identified as a structural motif of 100–130 amino acids in the WHSC1 protein and predicted to be protein-protein interaction domain. It belongs Tudor 'Royal Family', which consists Tudor, chromodomain, MBT domains. While chromodomain domains have long been known bind methylated histones, shown exhibit histone binding ability only until recently. Methodology/Principal Findings has DNA domain, but sequence analysis previous studies show that exhibits...
Arginine methylation modulates diverse cellular processes and represents a molecular signature of germ-line-specific Piwi family proteins. A subset Tudor domains recognize arginine modifications, but the binding mechanism has been lacking. Here we establish that, like other germ-line proteins, ancestral staphylococcal nuclease domain-containing 1 (SND1) polypeptide is expressed associates with PIWIL1/Miwi in germ cells. We find that human SND1 binds PIWIL1 an methylation-dependent manner...
In this paper, we study online deadline-aware task dispatching and scheduling in edge computing. We jointly consider management of the networking bandwidth computing resources to meet maximum number deadlines. propose an algorithm Dedas, which greedily schedules newly arriving tasks considers whether replace some existing order make new deadlines satisfied. derive a non-trivial competitive ratio theoretically, our analysis is asymptotically tight. then build DeEdge, testbed installed with...
CFP1 is a CXXC domain-containing protein and an essential component of the SETD1 histone H3K4 methyltransferase complex. domain proteins direct different chromatin-modifying activities to various chromatin regions. Here, we report crystal structures in complex with six CpG DNA sequences. The crescent-shaped wedged into major groove DNA, distorting B-form interacts extensively DNA. elucidate molecular mechanism non-methylated CpG-binding specificity domain. motif confined by tripeptide...
H3K27me3 deposition at FLC requires FCA- COOLAIR binding; SSU72 antagonizes this binding, thus reducing .
N(6)-methyladenosine (m(6)A) is the most common reversible internal modification in mammalian RNA. Changes m(6)A levels have been implicated a variety of cellular processes, including nuclear RNA export, control protein translation, and splicing, they linked to obesity, cancer, other human diseases. METTL3 METTL14 are N(6)-adenosine methyltransferases that work more efficiently stable METTL3-METTL14 heterodimer complex (METTL3-14). ALKBH5 an m(6)A-RNA demethylase belongs AlkB family...
Numerous RNAs are exported from the nucleus, abnormalities of which lead to cellular complications and diseases. How thousands circular (circRNAs) nucleus remains elusive. Here, we provide lines evidence demonstrate a link between conserved Exportin 4 (XPO4) nuclear export subset circRNAs in metazoans. Exonic (ecircRNAs) with higher expression levels, larger length, lower GC content more sensitive XPO4 deficiency. Cellular insufficiency leads circRNA accumulation, circRNA:DNA (ciR-loop)...
Human brahma-related gene 1 (Brg1) is a core protein in human SWI/SNF chromatin-remodeling complex which regulates expression. Brg1 contains bromodomain that has been shown to anchor the entire promoter nucleosomes by interacting with histones are acetylated at specific lysine residues. The belongs an important subclass of family for no structural information known. Here we report solution structure determined NMR. conserves left-handed, four-helix bundle topology found other structures....
Abstract A large number of structurally diverse epigenetic reader proteins specifically recognize methylated lysine residues on histone proteins. Here we describe comparative thermodynamic, structural and computational studies recognition the positively charged natural trimethyllysine its neutral analogues by This work provides experimental theoretical evidence that predominantly via a combination favourable cation– π interactions release high-energy water molecules occupy aromatic cage...
In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to heptameric Sm ring for assembly on pre-snRNAs at conserved site [A(U) 4–6 G]. Gemin5, a WD40 protein component SMN complex, is responsible recognizing pre-snRNAs. addition, Gemin5 has been reported specifically bind m 7 G cap. this study, we show that domain both necessary and sufficient binding by isothermal titration calorimetry (ITC) mutagenesis assays. We further determined crystal...