A5102715514- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- RNA Research and Splicing
- Trypanosoma species research and implications
- Enzyme Structure and Function
- Protein Degradation and Inhibitors
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- Biochemical and Molecular Research
- Epigenetics and DNA Methylation
- Dendrimers and Hyperbranched Polymers
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Computational Drug Discovery Methods
- Cellular transport and secretion
- Chemical Synthesis and Analysis
- Advanced Polymer Synthesis and Characterization
- Protein Kinase Regulation and GTPase Signaling
- Fungal and yeast genetics research
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Bacterial Genetics and Biotechnology
Hefei National Center for Physical Sciences at Nanoscale
2015-2024
University of Science and Technology of China
2015-2024
Ministry of Education of the People's Republic of China
2023
Center for Excellence in Molecular Cell Science
2021
Hefei Institutes of Physical Science
2018
Hefei University
2005-2017
Center for Life Sciences
2016
Microscale (United States)
2012
East China Normal University
2010
Anhui University
2010
Human brahma-related gene 1 (Brg1) is a core protein in human SWI/SNF chromatin-remodeling complex which regulates expression. Brg1 contains bromodomain that has been shown to anchor the entire promoter nucleosomes by interacting with histones are acetylated at specific lysine residues. The belongs an important subclass of family for no structural information known. Here we report solution structure determined NMR. conserves left-handed, four-helix bundle topology found other structures....
Histone acetylation is a hallmark for gene transcription. As histone acetyltransferase, MOZ (monocytic leukemia zinc finger protein) important HOX expression as well embryo and postnatal development. In vivo, forms tetrameric complex with other subunits, including several chromatin-binding modules regulatory functions. Here we report the solution structure of tandem PHD (plant homeodomain) (PHD12) human in free state 1.47 Å crystal H3K14ac peptide, which reveals structural basis recognition...
Abstract The E3 ligase-degron interaction determines the specificity of ubiquitin‒proteasome system. We recently discovered that FEM1B, a substrate receptor Cullin 2-RING ligase (CRL2), recognizes C-degrons containing C-terminal proline. By solving several cryo-EM structures CRL2 FEM1B bound to different C-degrons, we elucidate dimeric assembly complex. Furthermore, reveal distinct dimerization states unmodified and neddylated uncover NEDD8-mediated activation mechanism . Our research also...
Pup (prokaryotic ubiquitin-like protein) from Mycobacterium tuberculosis is the first protein identified in non-eukaryotic cells. Although different proteins eukaryotes share low sequence similarity, their 3D (three-dimensional) structures exhibit highly conserved typical folds. Interestingly, our studies reveal that not only shares but also presents a totally distinguished structure compared with other superfamily proteins. Diverse predictions combined CD and NMR spectroscopic all...
The plant homeodomain finger 6 (PHF6) was originally identified as the gene mutated in X-linked mental retardation disorder Börjeson-Forssman-Lehmann syndrome. Mutations PHF6 have also been associated with T-cell acute lymphoblastic leukemia and myeloid leukemia. Approximately half of disease-associated mutations are distributed second conserved extended (ePHD2) PHF6, indicating functional importance ePHD2 domain. Here, we report high resolution crystal structure domain which contains an...
SUV39H1, the first identified histone lysine methyltransferase in human, is involved chromatin modification and gene regulation. SUV39H1 contains a chromodomain its N-terminus, which potentially plays role methyl-lysine recognition targeting. In this study, structure of human was determined by X-ray crystallography. The displays generally conserved fold compared with other solved chromodomains. However, different from chromodomains, possesses much longer helix at C-terminus. Furthermore,...
Hfq is a bacterial post-transcriptional regulator. It facilitates base-pairing between sRNA and target mRNA. mediates DsrA-dependent translational activation of rpoS mRNA at low temperatures. encodes the stationary-phase σ factor S , which central regulator in general stress response. However, structural information on Hfq–DsrA interaction not yet available. Although reported to hydrolyze ATP, ATP-binding site still unknown. Here, we report ternary crystal complex structure Escherichia coli...
A convenient approach for the high-throughput screening of dendrimer-binding drugs by NMR techniques including saturation transfer difference (STD) and Hadamard-encoded nuclear Overhauser effect measurements is presented. The results insoluble show that phenylbutazone sulfamethoxazole prefer to localize in interior pockets dendrimer, while mycophenolic acid mostly binds on dendrimer surface, noncharged like trimethoprim primidone do not interact with dendrimers. In another path soluble...
Methylation of H4K20 (Lys(20) histone H4) plays an important role in the regulation diverse cellular processes. In fission yeast, all three states methylation are catalysed by Set9. Pdp1 is a PWWP (proline-tryptophan-tryptophan-proline) domain-containing protein, which associates with Set9 to regulate its chromatin localization and methyltransferase activity towards H4K20. The structure domain, first domain identified binds methyl-lysine at site, was determined present study solution NMR....
Fully acetylated poly(amidoamine) (PAMAM) dendrimer was proposed as a biocompatible drug vehicle using dexamethasone 21-phosphate (Dp21) model drug. NMR techniques including 1H and 2D NOE were used to characterize the host−guest chemistry of dendrimer/Dp21 cationic complexes. The pH-dependent micellization, complexation, inclusion behaviors Dp21 observed in presence PAMAM dendrimers. Acetylated only encapsulates at acidic conditions, while can host both neutral conditions. orientation...
Abstract Motivation The ABACUS (a backbone-based amino acid usage survey) method uses unique statistical energy functions to carry out protein sequence design. Although some of its results have been experimentally verified, accuracy remains improvable because several important components the not specifically optimized for design or in contexts other parts method. computational efficiency also needs be improved support interactive online applications consideration a large number alternative...
Protein modifiers are involved in diverse biological processes and regulate the activity or function of target proteins by covalently conjugating to them. Although ubiquitin a number ubiquitin-like protein (Ubls) eukaryotes have been identified, no modifier has found prokaryotes; thus, their evolutionary origin remains puzzle. To infer relationships between sulfur carrier proteins, we solved solution NMR structure Urm1 (ubiquitin-related modifier-1) from Saccharomyces cerevisiae. Both...
Abstract Background Brd2 is a transcriptional regulator and belongs to BET family, less characterized novel class of bromodomain-containing proteins. contains two tandem bromodomains (BD1 BD2, 46% sequence identity) in the N-terminus conserved motif named ET (extra C-terminal) domain at C-terminus that also present some other bromodomain The have been shown bind acetylated histone H4 be responsible for mitotic retention on chromosomes, which probably distinctive feature family Although...
Brd4 belongs to the BET family. It is a multifunctional protein involved in transcription, replication, signal transduction pathway, and cell cycle progression. All of these functions are linked its association with acetylated chromatin. With tandem bromodomains, avidly binds diacetylated H4-AcK5/K12 H3-AcK9/K14 peptides. Solution structure second bromodomain (BD) reported this research. In addition piD-helix, which special for members, an incompact alphaZ' distinct from Brd2 BD2 found,...
Stable transmission of genetic information during cell division requires faithful mitotic spindle assembly and chromosome segregation. The Ran GTPase plays a key role in assembly. However, how the generation chemical gradient Ran-GTP at is coupled to post-translational modifications has never been characterized. Here, we solved complex structure with nucleotide release factor Mog1 delineated novel mitosis-specific acetylation-regulated Ran–Mog1 interaction Our structure-guided functional...