A5100754890- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Enzyme Structure and Function
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- GaN-based semiconductor devices and materials
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Advanced Chemical Physics Studies
- Viral gastroenteritis research and epidemiology
- Surface and Thin Film Phenomena
- Bacterial Genetics and Biotechnology
- Semiconductor Quantum Structures and Devices
- Metal and Thin Film Mechanics
- Influenza Virus Research Studies
- Acoustic Wave Resonator Technologies
- Virus-based gene therapy research
- Ultrasound and Hyperthermia Applications
- Animal Virus Infections Studies
- Pain Mechanisms and Treatments
- ZnO doping and properties
University of Science and Technology of China
2021-2025
First Affiliated Hospital of Chengdu Medical College
2025
Anhui Provincial Center for Disease Control and Prevention
2025
Sun Yat-sen University
2019-2025
Sun Yat-sen University Cancer Center
2019-2025
State Key Laboratory of Oncology in South China
2019-2024
Taiyuan Central Hospital
2008-2024
Henan Agricultural University
2023-2024
Shanxi Medical University
2024
Sichuan University of Science and Engineering
2024
Scaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach limited by need to forward-design specific Watson-Crick base pairing manually for any given target structure. Here, we report general, top-down strategy design nearly arbitrary architectures autonomously based only on shape. Objects are represented as closed surfaces rendered polyhedral networks parallel duplexes, which enables complete scaffold routing with spanning tree...
The development of a safe and effective SARS-CoV-2 vaccine is public health priority. We designed subunit candidates using self-assembling ferritin nanoparticles displaying one two multimerized spikes: full-length ectodomain (S-Fer) or C-terminal 70 amino-acid deletion (SΔC-Fer). Ferritin an attractive nanoparticle platform for production vaccines, ferritin-based vaccines have been investigated in humans separate clinical trials. confirmed proper folding antigenicity spike on the surface by...
Abstract N 6 -methyladenosine (m A) is the most abundant ribonucleotide modification among eukaryotic messenger RNAs. The m A “writer” consists of catalytic subunit A-METTL complex (MAC) and regulatory A-METTL-associated (MACOM), latter being essential for enzymatic activity. Here, we report cryo-electron microscopy (cryo-EM) structures MACOM at a 3.0-Å resolution, uncovering that WTAP VIRMA form core structure ZC3H13 stretches conformation by binding VIRMA. Furthermore, 4.4-Å resolution...
Abstract Histamine receptors are a group of G protein-coupled (GPCRs) that play important roles in various physiological and pathophysiological conditions. Antihistamines target the histamine H 1 receptor (H R) have been widely used to relieve symptoms allergy inflammation. Here, uncover details regulation R by known second-generation antihistamines, thereby providing clues for rational design newer we determine cryo-EM structure apo form bound different antihistamines. In addition deep...
Paclitaxel is widely used in cancer treatments, but poor water-solubility and toxicity raise serious concerns. Here we report an RNA four-way junction nanoparticle with ultra-thermodynamic stability to solubilize load paclitaxel for targeted therapy. Each covalently loads twenty-four molecules as a prodrug. The RNA-paclitaxel complex structurally rigid stable, demonstrated by the sub-nanometer resolution imaging of cryo-EM. Using nanoparticles carriers increases 32,000-fold. Intravenous...
Specimens below 50 kDa have generally been considered too small to be analyzed by single-particle cryo-electron microscopy (cryo-EM). The high flexibility of pure RNAs makes it difficult obtain high-resolution structures cryo-EM. In bacteria, riboswitches regulate sulfur metabolism through binding the S-adenosylmethionine (SAM) ligand and offer compelling targets for new antibiotics. SAM-I, SAM-I/IV, SAM-IV are three most commonly found SAM riboswitches, but structure is still unknown. Here,...
3D polyhedral wireframe DNA nanoparticles (DNA-NPs) fabricated using scaffolded origami offer complete and independent control over NP size, structure, asymmetric functionalization on the 10–100 nm scale. However, complex sequence design needed for synthesis of these versatile DNA-NPs has limited their widespread use to date. While automated algorithms DAEDALUS vHelix-BSCOR apply synthesized either uniformly dual or hybrid single-dual duplex edges, respectively, are relatively compliant...
Abstract This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess quality models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate reproducibility results different software developers and users (3) compare performance metrics used for model evaluation, particularly Fit-to-Map metrics, with focus on near-atomic resolution. Our findings demonstrate relatively high accuracy cryo-EM derived...
Background: Ferroptosis, a regulated cell death which is driven by the iron-dependent peroxidation of lipids, plays an important role in cancer. However, studies about ferroptosis-related Long non-coding RNAs (lncRNAs) breast cancer (BC) are limited. Besides, prognostic lncRNAs and their relationship to immune microenvironment remain unclear. This study aimed explore potential value Methods: RNA-sequencing data female patients were downloaded from TCGA database. 937 randomly separated into...
Assembly-line polyketide synthases, such as the 6-deoxyerythronolide B synthase (DEBS), are large enzyme factories prized for their ability to produce specific and complex products. By channeling protein-tethered substrates across multiple active sites in a defined linear sequence, these enzymes facilitate programmed small-molecule syntheses that could theoretically be harnessed access countless product structures. Using cryogenic electron microscopy study DEBS module 1, we present...
Numerous RNAs are exported from the nucleus, abnormalities of which lead to cellular complications and diseases. How thousands circular (circRNAs) nucleus remains elusive. Here, we provide lines evidence demonstrate a link between conserved Exportin 4 (XPO4) nuclear export subset circRNAs in metazoans. Exonic (ecircRNAs) with higher expression levels, larger length, lower GC content more sensitive XPO4 deficiency. Cellular insufficiency leads circRNA accumulation, circRNA:DNA (ciR-loop)...
Abstract The first RNA category of the Critical Assessment Techniques for Structure Prediction competition was only made possible because scientists who provided experimental structures to challenge predictors. In this article, these offer a unique and valuable analysis both successes areas improvement in predicted models. All 10 RNA‐only targets yielded predictions topologically similar experimentally determined structures. For one target, experimentalists were able phase their x‐ray...
Abstract Hybrid RNA:DNA origami, in which a long RNA scaffold strand folds into target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to limited extent despite its unique potential for biomedical delivery of mRNA, tertiary structure characterization RNAs, and fabrication artificial ribozymes. Here, we investigate design principles three-dimensional wireframe RNA-scaffolded origami rendered as polyhedra composed dual-duplex edges. We computationally...
Abstract Vitamin C (L-ascorbic acid) is an essential nutrient for human health, and its deficiency has long been known to cause scurvy. Sodium-dependent vitamin transporters (SVCTs) are responsible uptake tissue distribution in mammals. Here, we present cryogenic electron microscopy structures of mouse SVCT1 both the apo substrate-bound states. Mouse forms a homodimer with each protomer containing core domain gate domain. The tightly packed extracellular interfaces between stabilize protein...
Abstract Nipah virus (NiV) is a World Health Organization priority pathogen and there are currently no approved drugs for clinical immunotherapy. Through the use of naïve human phage-displayed Fab library, two neutralizing antibodies (NiV41 NiV42) targeting NiV receptor binding protein (RBP) were identified. Following affinity maturation, derived from NiV41 display cross-reactivity against both Hendra (HeV), whereas antibody based on NiV42 only specific to NiV. Results immunogenetic analysis...
The stability and function of biomolecules are directly influenced by their myriad interactions with water. In this study, we investigated water through cryogenic electron microscopy (cryo-EM) on a highly solvated molecule, the Tetrahymena ribozyme, determined at 2.2 2.3 Å resolutions. By employing segmentation-guided ion modeling (SWIM), an approach combining resolvability chemical parameters, automatically modeled cross-validated molecules Mg 2+ ions in ribozyme core, revealing extensive...
RNA tetrahedral nanoparticles with two different sizes are successfully assembled by a one-pot bottom-up approach high efficiency and thermal stability. The reported design principles can be extended to construct higher-order polyhedral architectures for various applications such as targeted cancer imaging therapy. As service our authors readers, this journal provides supporting information supplied the authors. Such materials peer reviewed may re-organized online delivery, but not...