A5052805211- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Genetics and Neurodevelopmental Disorders
- Protein Degradation and Inhibitors
- Pluripotent Stem Cells Research
- Remote Sensing in Agriculture
- Metabolomics and Mass Spectrometry Studies
- interferon and immune responses
- Acute Myeloid Leukemia Research
- Angiogenesis and VEGF in Cancer
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Remote-Sensing Image Classification
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Traditional Chinese Medicine Analysis
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Bone Metabolism and Diseases
- Glycosylation and Glycoproteins Research
- Nuclear Structure and Function
China Agricultural University
1998-2025
University of Alabama at Birmingham
2011-2024
Virginia Commonwealth University
2022-2024
University of Alabama
2014
St. Vincent's Birmingham
2013
University of North Carolina at Chapel Hill
2001-2007
Howard Hughes Medical Institute
2007
Kyushu University
2004
UNC Lineberger Comprehensive Cancer Center
2002
Segeberger Kliniken
2002
Polycomb group (PcG) proteins play important roles in maintaining the silent state of HOX genes. Recent studies have implicated histone methylation long-term gene silencing. However, a connection between PcG-mediated silencing and has not been established. Here we report purification characterization an EED-EZH2 complex, human counterpart Drosophila ESC-E(Z) complex. We demonstrate that complex specifically methylates nucleosomal H3 at lysine 27 (H3-K27). Using chromatin immunoprecipitation...
The Polycomb group (PcG) protein Eed is implicated in regulation of imprinted X-chromosome inactivation extraembryonic cells but not random X embryonic cells. Drosophila homolog the Eed-Ezh2 PcG complex achieves gene silencing through methylation histone H3 on lysine 27 (H3-K27), which suggests a role for H3-K27 inactivation. Here we demonstrate that transient recruitment to inactive chromosome (Xi) occurs during initiation both and accompanied by methylation. Recruitment Xi depend Xist RNA...
Acetylation of core histone tails plays a fundamental role in transcription regulation. In addition to acetylation, other posttranslational modifications, such as phosphorylation and methylation, occur tails. Here, we report the purification, molecular identification, functional characterization H4–specific methyltransferase PRMT1, protein arginine methyltransferase. PRMT1 specifically methylates 3 ( Arg ) H4 vitro vivo. Methylation by facilitates subsequent acetylation p300. However,...
The amino-terminal histone tails are subject to covalent post-translational modifications such as acetylation, methylation, and phosphorylation. In the code hypothesis, these exposed unstructured accessible a repertoire of regulatory factors that specifically recognize various modified histones, thereby generating altered chromatin structures mediate specific biological responses. Here, we report lysine (Lys) 79 H3, which resides in globular domain, is methylated eukaryotic organisms. yeast...
The frequent occurrence of crop pests and diseases is one the important factors leading to reduction quality yield. Since are characterized by high similarity fast movement, this poses a challenge for artificial intelligence techniques identify in timely accurate manner. Therefore, we propose new high-precision real-time method maize pest detection, Maize-YOLO. network based on YOLOv7 with insertion CSPResNeXt-50 module VoVGSCSP module. It can improve detection accuracy speed while reducing...
The polycomb repressive complex 2 (PRC2) is the major methyltransferase for H3K27 methylation, a modification critical maintaining repressed gene expression programs throughout development. It has been previously shown that PRC2 maintains histone methylation patterns during DNA replication in part through its ability to bind H3K27me3. However, mechanism by which recognizes H3K27me3 unclear. Here we show WD40 domain of EED, component, methyllysine histone-binding domain. crystal structures...
Members of the FOXO (forkhead O) class transcription factors are tumor suppressors that also control aging and organismal life span. Mammalian degradation is proteasome-mediated, although ubiquitin E3 ligase for remains to be defined. We show MDM2 binds FOXO1 FOXO3A promotes their ubiquitination degradation, a process apparently dependent on phosphorylation at AKT sites activity MDM2. Binding occurs through region directs its cellular trafficking forkhead box FOXO1. in cell-free system,...
Polycomb group (PcG) proteins are required for maintaining the silent state of homeotic genes and other important developmental regulators. The silencing function PcG has been linked to their intrinsic histone modifying enzymatic activities. EED-EZH2 complex, containing core subunits EZH2, EED, SUZ12, RbAp48, functions as a H3K27-specific methyltransferase. Here we describe identification characterization related protein complex which is distinguished from previous by presence another...
RBM15, an RNA binding protein, determines cell-fate specification of many tissues including blood. We demonstrate that RBM15 is methylated by protein arginine methyltransferase 1 (PRMT1) at residue R578, leading to its degradation via ubiquitylation E3 ligase (CNOT4). Overexpression PRMT1 in acute megakaryocytic leukemia cell lines blocks megakaryocyte terminal differentiation downregulation level. Restoring level rescues blocked overexpression. At the molecular level, binds pre-messenger...
Polycomb repressive complex two (PRC2) has been implicated in embryonic stem (ES) cell pluripotency; however, the mechanistic roles of this are unclear. It was assumed that ES cells contain PRC2 with same subunit composition as identified HeLa and Drosophila embryos. Here, we report mouse contains at least three additional subunits: JARID2, MTF2, a novel protein denoted esPRC2p48. esPRC2p48 highly expressed compared to differentiated cells. Importantly, knockdowns or alter level...
Abstract Blue light (BL)-dependent H+ pumping by guard cells, which drives stomatal opening, is inhibited abscisic acid (ABA). We investigated this response with respect to the activity of plasma membrane H+-ATPase using Vicia cell protoplasts. ATP hydrolysis H+-ATPase, phosphorylation and binding 14-3-3 protein stimulated BL were ABA at 10 μ m. All these responses similarly hydrogen peroxide (H2O2) 1 mm. The ABA-induced inhibitions BL-dependent partially restored ascorbate, an intracellular...
Post-translational histone modifications play important roles in regulating gene expression programs, which turn determine cell fate and lineage commitment during development. One such modification is ubiquitination, primarily targets H2A H2B. Although ubiquitination of H2B has been generally linked to silencing activation, respectively, the functions eukaryote development are not well understood. Here, we identified USP12 USP46 as deubiquitinases that regulate Xenopus prefer nucleosomal...
Post-translational histone modifications play important roles in regulating chromatin structure and function. Histone H2B ubiquitination deubiquitination have been implicated transcriptional regulation, but the function of is not well defined, particularly higher eukaryotes. Here we report purification ubiquitin-specific peptidase 49 (USP49) as a H2B-specific deubiquitinase demonstrate that by USP49 required for efficient cotranscriptional splicing large set exons. forms complex with...
Neural crest cells arise from the border of neural plate and epidermal ectoderm, migrate extensively differentiate into diverse cell types during vertebrate embryogenesis. Although much has been learnt about growth factor signals gene regulatory networks that regulate development, limited information is available on how epigenetic mechanisms control this process. In study, we show Polycomb repressive complex 2 (PRC2) cooperates with transcription Snail2/Slug to modulate development in...