A5066038194- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Cancer-related Molecular Pathways
- Bone Metabolism and Diseases
- Melanoma and MAPK Pathways
- Health and Wellbeing Research
- Cell Adhesion Molecules Research
- Cancer Genomics and Diagnostics
- melanin and skin pigmentation
- Protease and Inhibitor Mechanisms
- CRISPR and Genetic Engineering
- Bone health and treatments
- Chromatin Remodeling and Cancer
- DNA Repair Mechanisms
- Prostate Cancer Treatment and Research
- Education and Learning Interventions
- Cytokine Signaling Pathways and Interactions
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
Korea National Sport University
2022-2025
University of Southern California
2015-2024
Southern California University for Professional Studies
2023
USC Norris Comprehensive Cancer Center
2007-2013
Samjin Pharm (South Korea)
2013
King Faisal Specialist Hospital & Research Centre
2013
Dongnam Institute of Radiological & Medical Sciences
2011
Broad Center
2009-2011
Rockefeller University
2000-2008
University of Rochester
2001
Although limited proteolysis of the histone H3 N-terminal tail (H3NT) is frequently observed during mammalian differentiation, specific genomic sites targeted for H3NT and functional significance cleavage remain largely unknown. Here we report first method to identify examine H3NT-cleaved regions in mammals, called chromatin immunoprecipitation (ChIP) acetylated (ChIPac). By applying ChIPac combined with deep sequencing (ChIPac-seq) an established cell model osteoclast discovered that...
macroH2A (mH2A) is an unusual histone variant consisting of a H2A-like domain fused to large nonhistone region. In this work, we show that mH2A represses p300- and Gal4-VP16-dependent polymerase II transcription, have dissected the mechanism by which repression realized. The repressive effect observed at level initiation but not elongation interferes with p300-dependent acetylation. region responsible for both transcription inhibition addition, presence within nucleosome able block...
Linker histone H1 has been generally viewed as a global repressor of transcription by preventing the access factors to sites in chromatin. However, recent studies suggest that can interact with other regulatory for its action negative modulator specific genes. To investigate these aspects, we established human cell line expressing H1.2, one subtypes, purification H1-interacting proteins. Our results showed H1.2 stably associate sets cofactors and ribosomal proteins significantly repress...
The incorporation of histone variants into nucleosomes is one the main strategies that cell uses to regulate structure and function chromatin. Histone H2A.Z an evolutionarily conserved H2A variant preferentially localized within at transcriptional start site (TSS). reorganizes local chromatin recruits machinery for gene activation. High expression has been reported in several types cancers causally linked genomic instability tumorigenesis. However, it not entirely clear how overexpression...
SET and MYND domain containing protein 3 (SMYD3) is a histone methyltransferase, which has been implicated in cell growth cancer pathogenesis. Increasing evidence suggests that SMYD3 can influence distinct oncogenic processes by acting as gene-specific transcriptional regulator. However, the mechanistic aspects of transactivation whether acts concert with other transcription modulators remain unclear. Here, we show interacts human positive coactivator 4 (PC4) such interaction potentiates...
Abstract Our recent work has shown that DCAF1 (also known as VprBP) is overexpressed in colon cancer and phosphorylates histone H2AT120 to drive epigenetic gene inactivation oncogenic transformation. We have extended these observations by investigating whether also non-histone proteins an additional mechanism linking its kinase activity development. now demonstrate EZH2 at T367 augment nuclear stabilization enzymatic cells. Consistent with this mechanistic role, DCAF1-mediated...
A global transcriptional co-activator, the SNF/SWI complex, has been characterized as a chromatin remodeling factor that enhances accessibility of machinery to DNA within repressive structure. On other hand, mutations in some human complex components have linked tumor formation. We show here SYT, partner protein generating synovial sarcoma fusion SYT-SSX, associates with native complexes. The SYT unique QPGY domain, which is also present largest subunits, p250 and newly identified homolog...
H2A.Z is an evolutionarily conserved H2A variant that plays a key role in the regulation of chromatin transcription. To understand molecular mechanism exchange, we purified two distinct H2A.Z-interacting complexes termed small and big from human cell line. The complex contains most components SRCAP remodeling TIP60 HAT complexes, whereas possesses only subset subunits. Our exchange analysis revealed both enhance incorporation H2A.Z-H2B dimer into nucleosome. In addition, TIP60-mediated...
DNA methyltransferases (DNMTs) play an important role in establishing and maintaining methylation. Aberrant expression of DNMTs their isoforms has been found many types cancer, contribution to aberrant methylation proposed. Here, we generated HEK 293T cells stably transfected with each 13 different (DNMT1, two DNMT3A isoforms, nine DNMT3B DNMT3L) assessed the changes induced by DNMT. We obtained profiles repetitive elements 1505 CpG sites from 808 cancer-related genes. that have specific...
Increasing evidence suggests that linker histone H1 can influence distinct cellular processes by acting as a gene-specific regulator. However, the mechanistic basis underlying such specificity and whether acts in concert with other chromatin-altering activities remain unclear. Here, we show one of subtypes, H1.2, stably interacts Cul4A E3 ubiquitin ligase PAF1 elongation complexes interaction potentiates target gene transcription via induction H4K31 ubiquitylation, H3K4me3, H3K79me2. Cul4A,...
Calcium/calmodulin-dependent protein kinase II (CaMKII) plays a central role in pathological cardiac hypertrophy, but the mechanisms by which it modulates gene activity nucleus to mediate hypertrophic signaling remain unclear. Here, we report that nuclear CaMKII activates transcription directly binding chromatin and regulating phosphorylation of histone H3 at serine-10. These specific activities are demonstrated both vitro primary neonatal rat cardiomyocytes. Activation agonists increases...
Abstract Linker histone H1 is a protein component of chromatin and has been linked to higher-order compaction global gene silencing. However, growing body evidence suggests that plays gene-specific role, regulating relatively small number genes. Here we show H1.2, one the subtypes, overexpressed in cancer cells contributes H1.2 gets recruited distinct regions manner dependent on EZH2-mediated H3K27me3 inhibits transcription multiple growth suppressive genes via modulation architecture. The...