A5079297450- Ion channel regulation and function
- SARS-CoV-2 and COVID-19 Research
- Renal and related cancers
- Renal cell carcinoma treatment
- Advanced biosensing and bioanalysis techniques
- Mitochondrial Function and Pathology
- Cardiac electrophysiology and arrhythmias
- COVID-19 Clinical Research Studies
- RNA and protein synthesis mechanisms
- Meat and Animal Product Quality
- Network Traffic and Congestion Control
- Photosynthetic Processes and Mechanisms
- Network Packet Processing and Optimization
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Ion Transport and Channel Regulation
- CRISPR and Genetic Engineering
- Lipid Membrane Structure and Behavior
- Animal Virus Infections Studies
- Enzyme Structure and Function
- Cell death mechanisms and regulation
- Nanopore and Nanochannel Transport Studies
- Electrochemical Analysis and Applications
Westlake University
2020-2025
Peking University
2025
Fujian Provincial Hospital
2025
Fujian Medical University
2025
Fuzhou University
2025
Chongqing Jiaotong University
2025
Sun Yat-sen Memorial Hospital
2023-2024
East China Normal University
2008-2024
Zhongda Hospital Southeast University
2019-2024
Ministry of Natural Resources
2024
Voltage-gated sodium (Nav) channels are responsible for the initiation and propagation of action potentials. They associated with a variety channelopathies targeted by multiple pharmaceutical drugs natural toxins. Here, we report cryogenic electron microscopy structure putative Nav channel from American cockroach (designated NavPaS) at 3.8 angstrom resolution. The voltage-sensing domains (VSDs) four repeats exhibit distinct conformations. entrance to asymmetric selectivity filter vestibule...
The voltage-gated calcium channel Ca(v)1.1 is engaged in the excitation-contraction coupling of skeletal muscles. complex consists pore-forming subunit α1 and auxiliary subunits α2δ, β, γ. We report structure rabbit determined by single-particle cryo-electron microscopy. four homologous repeats are arranged clockwise extracellular view. γ subunit, whose resembles claudins, interacts with voltage-sensing domain repeat IV (VSD(IV)), whereas cytosolic β located adjacent to VSD(II) α1. α2 loops...
Gating a calcium channel The type 2 ryanodine receptor (RyR2) controls the release of ions from sarcoplasmic reticulum in cardiac cells—the initiating step muscle contraction. Mutations RyR2 are associated with diseases. Peng et al. used single-particle electron cryomicroscopy to determine structure porcine heart at 4.4-Å resolution closed and 4.2-Å open. structures reveal how interdomain motions result conformational change cytoplasmic region that is transduced by central domain cause open...
Animal toxins that modulate the activity of voltage-gated sodium (Nav) channels are broadly divided into two categories-pore blockers and gating modifiers. The pore tetrodotoxin (TTX) saxitoxin (STX) responsible for puffer fish shellfish poisoning in humans, respectively. Here, we present structures insect Nav channel NavPaS bound to a modifier toxin Dc1a at 2.8 angstrom-resolution presence TTX or STX 2.6-Å 3.2-Å resolution, inserts cleft between VSDII NavPaS, making key contacts with both...
The pandemic of COVID-19, caused by SARS-CoV-2, is a major global health threat. Epidemiological studies suggest that bats (Rhinolophus affinis) are the natural zoonotic reservoir for SARS-CoV-2. However, host range SARS-CoV-2 and intermediate hosts facilitate its transmission to humans remain unknown. interaction coronavirus with receptor key genetic determinant cross-species transmission. uses angiotensin-converting enzyme 2 (ACE2) as enter cells in species-dependent manner. In this study,...
Recent studies have suggested that microRNA biomarkers could be useful for stratifying lung cancer subtypes, but signatures varied between different populations. Squamous cell carcinoma (SCC) is one major subtype of urgently needs to aid patient management. Here, we undertook the first comprehensive investigation on in Chinese SCC patients.MicroRNA expression was measured cancerous and noncancerous tissue pairs strictly collected from patients (stages I-III), who had not been treated with...
RIP3 is an essential upstream kinase in necroptosis. The pseudokinase MLKL functions as a substrate of to mediate downstream signaling. molecular mechanism by which recognizes and phosphorylates remains unknown. Here, we report the crystal structures mouse domain, kinase-like binary complex between two. Both adopt canonical fold. Free exists active conformation, whereas MLKL-bound stabilized AMP-PNP inactive conformation. formation RIP3-MLKL complex, involving their respective N- C-lobes,...
Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. A signal is generated by analyte-induced probe desorption. To address nonspecific displacement and the false positive signal, we herein report covalently linked for adenosine triphosphate (ATP) detection. fluorophore amino dual modified was to carboxyl group GO with coupling efficiency of ∼50%. The linearity, specificity, stability, regeneration covalent sensor were...
The ryanodine receptors (RyRs) are intracellular calcium channels responsible for rapid release of Ca2+ from the sarcoplasmic/endoplasmic reticulum (SR/ER) to cytoplasm, which is essential excitation-contraction (E-C) coupling cardiac and skeletal muscles. near-atomic resolution structure closed RyR1 revealed molecular details this colossal channel, while long-range allosteric gating mechanism awaits elucidation. Here, we report cryo-EM structures rabbit in three conformations at about 4 Å...
Abstract The pandemic of Coronavirus Disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a major global health threat. Epidemiological studies suggest that bats are the natural zoonotic reservoir for SARS-CoV-2. However, host range SARS-CoV-2 and intermediate hosts facilitate its transmission to humans remain unknown. interaction with receptor key genetic determinant cross-species transmission. uses angiotensin-converting enzyme (ACE2) as enter...