A5101851921- Hepatitis B Virus Studies
- Cell death mechanisms and regulation
- Respiratory Support and Mechanisms
- Ultrasound in Clinical Applications
- Liver Disease Diagnosis and Treatment
- interferon and immune responses
- Viral Infections and Outbreaks Research
- Cancer-related Molecular Pathways
- Hepatitis C virus research
- RNA modifications and cancer
- Airway Management and Intubation Techniques
- Cytokine Signaling Pathways and Interactions
- Intracerebral and Subarachnoid Hemorrhage Research
- Nosocomial Infections in ICU
- Pneumonia and Respiratory Infections
- Cancer, Hypoxia, and Metabolism
- Intensive Care Unit Cognitive Disorders
- Abdominal Surgery and Complications
- Virus-based gene therapy research
- Lung Cancer Treatments and Mutations
- Acute Ischemic Stroke Management
- Traumatic Brain Injury and Neurovascular Disturbances
- RNA Interference and Gene Delivery
- Computational Drug Discovery Methods
- Medical Research and Treatments
Lianyungang Oriental Hospital
2018-2024
Nanjing Medical University
2020-2024
Xuzhou Medical College
2018-2024
The First People’s Hospital of Lianyungang
2019-2024
Shandong University
2009-2021
Inner Mongolia Medical University
2020
University of Pennsylvania
2016
Liaocheng People's Hospital
2016
Institute of Immunology
2013
International Drug Development
2013
TIPE2, the tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 (TNFAIP8L2), plays an essential role in maintaining immune homeostasis. It is highly expressed macrophages and negatively regulates inflammation through inhibiting Toll-like receptor signaling. In this paper, we utilized RAW264.7 cells stably transfected with a TIPE2 expression plasmid, as well TIPE2-deficient to study roles of LPS-induced nitric oxide (NO) urea production. The results showed that TIPE2-deficiency...
We aimed to evaluate and compare the diagnostic performance of RNA-mNGS DNA-mNGS workflow in bacterial pneumonia, fungal pneumonia tuberculosis.
Phenotypic switching of vascular smooth muscle cells (VSMCs) is known to play a key role in the development atherosclerosis. However, mechanisms that mediate VSMC phenotypic are unclear. We report here TIPE2, tumor necrosis factor (TNF) α-induced protein 8-like 2 (TNFAIP8L2), plays an atheroprotective by regulating VSMCs response oxidized low-density lipoprotein (ox-LDL) stimuli. TIPE2-deficient treated with ox-LDL expressed lower levels contractile proteins such as SMαA, SM-MHC and...
Tumor necrosis factor-alpha-induced protein-8 like-3 (TNFAIP8L3, TIPE3) is a newly discovered member of TNFAIP8 family and regarded as lipid second messenger transfer protein that promotes cancer. Yet the nature cells tissues express TIPE3 has not been determined. In this study, we examined expression in various murine human by immunohistochemistry quantitative PCR. We found was almost identical most organs from mice. cytoplasmic expressed preferentially epithelial-derived with secretory...
TIPE3 (TNFAIP8L3) is the transfer protein of phosphoinositide second messengers that promote cancer. Its role in breast cancer has not been evaluated. We report here was significantly upregulated human tissues as compared with adjacent non-tumor from same patients. The level invasive ductal carcinoma significant higher than situ (DCIS), and lymphatic metastasized Additionally, positively correlated epidermal growth factor receptor 2 (HER-2), expression high-invasive cell lines low-invasive...
Objective . To identify the clinical correlations between plasma growth differentiation factor-15 (GDF-15), skeletal muscle function, and acute wasting in ICU patients with mechanical ventilation. In addition, to investigate its diagnostic value for ICU-acquired weakness (ICU-AW) predictive 90-day survival mechanically ventilated patients. Methods 95 respiratory failure, who required ventilation therapy, were randomly selected among hospitalized from June 2017 January 2019. The GDF-15 level...
Abstract Background Mechanical power (MP) is the total energy released into entire respiratory system per minute which mainly comprises three components: elastic static power, Elastic dynamic and resistive power. However, to overcome resistance gas flow not key factor in causing lung injury, but (EP) generates baseline stretch of fibers overcomes elastance may be closely related ARDS severity. Thus, this study aimed investigate whether EP superior other ventilator variables for predicting...
Protein farnesyl transferase (FTase) catalyzes transfer of a 15-carbon group from diphosphate (FPP) to conserved cysteine in the C-terminal Ca(1)a(2)X motif range proteins, including oncoprotein H-Ras ("C" refers cysteine, "a" any aliphatic amino acid, and "X" acid) lipid chain interacts with, forms part peptide binding site. Previous studies have shown that biological function is ablated when it modified with lipids are 3-5 orders magnitude less hydrophobic than FPP. Here, we employed...
Farnesylation is an important post-translational modification essential for the proper localization and function of many proteins. Transfer farnesyl group from diphosphate (FPP) to proteins catalyzed by protein farnesyltransferase (FTase). We employed a library FPP analogues with range aryl groups substituting individual isoprene moieties examine some structural electronic properties transfer analogue peptide FTase. Analysis steady-state kinetics substrates revealed that multiple-turnover...
Non-Hodgkin’s lymphoma (NHL), which includes diffuse large B-cell (DLBCL) and peripheral T-cell lymphoma, is a refractory malignant tumor originated from the lymphatic system. TNFAIP8L2 (TIPE2 or necrosis-alpha-induced protein-8 like 2) negative regulator for inflammation an inhibitor carcinogenesis. However, whether TIPE2 plays role in lymphomagenesis unknown. In this study, we determined expression NHL by immunohistochemistry investigated its clinicopathological significance DLBCL. We...
A total of 121 patients with moderate to severe ARDS admitted the intensive care unit (ICU) from June 2017 April 2020 and treated invasive mechanical ventilation were sequentially included in this study. Their general information was collected, MP recorded at 0 h, 24 48 72 h after admission ICU. Professionally trained researchers performed LUS assessments. Patients divided into death survival groups according their 28-day prognosis. The trend four time points analyzed. receiver operating...
Abstract Knowing the storage variations in lakes and reservoirs are essential for water resources environmental management, especially regions facing scarcity. However, quantification of changes is limited by sparse in-situ observations spatial coverage space(air)-borne altimetric sensors that have been used conventionally retrieval. This hampers attribution analysis lake changes. Here, we combined long-term optical remote sensing multi-source terrain elevation data to derive monthly time...
AIM:To establish a mice model of hepatitis B by using HBV-transgenic mice, and to transfer HBV-specific cytotoxic T lymphocytes (CTL) induced from syngeneic BALB/c immunized eukaryotic expression vector containing HBV complete genome DNA. METHODS:HBV DNA was obtained digested pBR322-2HBV ligated with the pcDNA3.Recombinant pcDNA3-HBV identified restriction endonuclease assay transfected into human hepatoma cell line HepG2 lipofectin.ELISA used detect HBsAg in culture supernatant, RT-PCR...
To investigate the clinical correlations between mechanical power (MP) and lung ultrasound score (LUS), analyze their evaluation value of prognosis in patients with acute respiratory distress syndrome (ARDS).Patients moderate to severe ARDS, who underwent invasive ventilation admitted intensive care unit (ICU) Lianyungang Affiliated Hospital Xuzhou Medical University from January 2017 March 2019 were enrolled. The MP LUS recorded 0, 24, 48 72 hours after ICU admission. divided into death...
AIM:To evaluate the inhibitory effect of antisense phosphorothioate oligonucleotide (asON) complementary to initiator human telomerase catalytic subunit (hTERT) on growth hepatoma cells. METHODS:The as-hTERT was synthesized by using a DNA synthesizer.HepG2.2.15 cells were treated with at concentration 10 µmol/L.After 72 h, these obtained for detecting inhibition, activity methods MTT, TRAP-PCR-ELISA, respectively.BALB/c(nu/nu) mice injected HepG2.2.15 and human-nude model obtained.There...