A5019122793- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- Algal biology and biofuel production
- Renal and related cancers
- Cerebrospinal fluid and hydrocephalus
- Genetics, Aging, and Longevity in Model Organisms
- Hedgehog Signaling Pathway Studies
- ATP Synthase and ATPases Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Connective tissue disorders research
- Advanced Thermodynamics and Statistical Mechanics
- Mitochondrial Function and Pathology
- Genetic Syndromes and Imprinting
- Cellular Mechanics and Interactions
- Ion channel regulation and function
- Microfluidic and Bio-sensing Technologies
- Food Quality and Safety Studies
- RNA regulation and disease
- Hearing, Cochlea, Tinnitus, Genetics
- Ion Transport and Channel Regulation
- Coastal wetland ecosystem dynamics
- Lipid metabolism and biosynthesis
Center for Life Sciences
2017-2025
Tsinghua University
2016-2025
Qingdao National Laboratory for Marine Science and Technology
2016-2025
King Center
2017-2020
National Health and Family Planning Commission
2018
Ministry of Environment, Forests and Climate Change
2013
University of California, Los Angeles
2007
Joint Genome Institute
2007
Institut de Biologie Physico-Chimique
2007
Sorbonne Université
2007
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It model system for studying chloroplast-based photosynthesis, as well the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited common ancestor animals, but lost in plants. We sequenced ∼120-megabase nuclear genome performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated...
Cilia and flagella are dynamic organelles that assembled disassembled during cell differentiation, stress, the cycle. Although intraflagellar transport (IFT) is well documented to be responsible for of ciliary/flagellar precursors from body flagella, little known about molecular mechanisms mobilizing body-localized make them available organelle assembly or disassembling microtubule-based axoneme shortening. Here, we show Chlamydomonas kinesin-13 (CrKinesin-13), a member family microtubule...
Intraflagellar transport (IFT) trains, multimegadalton assemblies of IFT proteins and motors, traffic in cilia. To study how trains assemble, we employed fluorescence protein-tagged Chlamydomonas reinhardtii. IFT-A motor are recruited from the cell body to basal pool, assembled into move through cilium, disperse back body. In contrast this ‘open’ system, IFT-B retrograde reenter pool a portion is reused directly anterograde indicating ‘semi-open’ system. Similar systems were also observed...
In ciliated cells, two types of microtubules can be categorized – cytoplasmic and axonemal. It has been shown that axonemal tubulins come from a “cytoplasmic pool” during cilia regeneration. However, the identity regulation this “pool” is not understood. Previously, we have Chlamydomonas kinesin-13 (CrKin13) phosphorylated flagellar regeneration, required for proper assembly. Here, show CrKin13 regulates depolymerization to control After loss before were quickly depolymerized, which...
The transition zone (TZ) of the cilium/flagellum serves as a diffusion barrier that controls entry/exit ciliary proteins. Mutations TZ proteins disrupt function and lead to multiple human diseases. However, systematic regulation composition signaling-related processes by different is not completely understood. Here, we reveal loss TCTN1 in Chlamydomonas reinhardtii disrupts assembly wedge-shaped structures TZ. Proteomic analysis cilia from WT three mutants, tctn1, cep290, nphp4, shows unique...
Specification of organelle size is crucial for cell function, yet we know little about the molecular mechanisms that report and regulate growth steady-state dimensions. The biflagellated green alga Chlamydomonas requires continuous-length feedback to integrate multiple events support flagellar assembly disassembly at same time maintain sensory motility functions organelle. Although several length mutants have been characterized, requisite reporter has not identified. Previously, showed...
Intraflagellar transport (IFT) particles or trains are composed of IFT-A and IFT-B complexes. To assess the working mechanism complex in IFT ciliogenesis, we have analyzed ift43 mutants Chlamydomnonas conjunction with other subunits. An null mutant a partial deletion IFT43 conserved domain has no short flagella. The accumulate not only but also IFT-Ain flagella, which is contrast to an ift140 mutant. necessary sufficient for function IFT43. directly interacts IFT121 loss results instability...
Single-cell metabolite analysis plays an important role in biological study. While mass spectrometry is a powerful tool for identification and quantitation of metabolites, the low absolute analyte amounts single cell difficulty sampling represent significant challenges analysis. In this study, we developed effective method with simple procedure analyzing cells. A was driven to capillary tip through electro-migration, followed by releasing contents electroporation, into sealed small volume...
Chlamydomonas reinhardtii, a bi-flagellated green alga, is model organism for studies of flagella or cilia related activities including cilia-based signaling, flagellar motility and biogenesis. Calcium has been shown to be key regulator these cellular processes whereas the signaling pathways linking calcium functions are less understood. Calcium-dependent protein kinases (CDPKs), which present in plants but not animals, also ciliated microorganisms led us examine their possible mechanisms...
Cilia and flagella are dynamic organelles that undergo assembly disassembly during each cell cycle. They structurally polarized, the mechanisms by which these disassembled incompletely understood. Here, we show flagellar resorption occurs in two distinct phases of length-dependent regulation. A CDK-like kinase, encoded shortening 1 (FLS1), is required for normal rate only distal part flagellum. Mechanistically, loss function FLS1 prevents initial phosphorylation CALK, an aurora-like kinase...