A5079916943- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Light effects on plants
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Plant Stress Responses and Tolerance
- Spectroscopy and Quantum Chemical Studies
- Marine and coastal ecosystems
- Metal-Catalyzed Oxygenation Mechanisms
- Redox biology and oxidative stress
- Protist diversity and phylogeny
- Mass Spectrometry Techniques and Applications
- Metalloenzymes and iron-sulfur proteins
- Metabolomics and Mass Spectrometry Studies
- Enzyme Structure and Function
- Micro and Nano Robotics
- Machine Learning in Bioinformatics
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- ATP Synthase and ATPases Research
- Electrocatalysts for Energy Conversion
- Microbial Community Ecology and Physiology
Okayama University
2004-2025
University of Münster
2016-2025
Institute of Plant Biology and Biotechnology
2014-2025
Tel Aviv University
2021
Institute for Biodiversity
2017
Milbank Memorial Fund
2011-2012
Friedrich Schiller University Jena
2002-2009
Institut de Biologie Physico-Chimique
2005-2007
University of Pennsylvania
2004-2007
Joint Genome Institute
2007
Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It model system for studying chloroplast-based photosynthesis, as well the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited common ancestor animals, but lost in plants. We sequenced ∼120-megabase nuclear genome performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated...
Plants protect themselves against herbivory with a diverse array of repellent or toxic secondary metabolites. However, many herbivorous insects have developed counteradaptations that enable them to feed on chemically defended plants without apparent negative effects. Here, we present evidence larvae the specialist insect, Pieris rapae (cabbage white butterfly, Lepidoptera: Pieridae), are biochemically adapted glucosinolate–myrosinase system, major chemical defense their host plants. The...
The fungus Fusarium fujikuroi causes “bakanae” disease of rice due to its ability produce gibberellins (GAs), but it is also known for producing harmful mycotoxins. However, the genetic capacity whole arsenal natural compounds and their role in fungus' interaction with remained unknown. Here, we present a high-quality genome sequence F. that was assembled into 12 scaffolds corresponding chromosomes described fungus. We used along ChIP-seq, transcriptome, proteome, HPLC-FTMS-based metabolome...
The unicellular green alga Chlamydomonas reinhardtii is a prime model for deciphering processes occurring in the intracellular compartments of photosynthetic cell. Organelle-specific proteomic studies have started to delineate its various subproteomes, but sequence-based prediction software necessary assign proteins subcellular localizations at whole genome scale. Unfortunately, existing tools are oriented toward land plants and tend mispredict localization nuclear-encoded algal proteins,...
Abstract Background Biogeochemical elemental cycling is driven by primary production of biomass via phototrophic phytoplankton growth, with 40% marine productivity being assigned to diatoms. Phytoplankton growth widely limited the availability iron, an essential component photosynthetic apparatus. The oceanic diatom Thalassiosira oceanica shows a remarkable tolerance low-iron conditions and was chosen as model for deciphering cellular response upon shortage this micronutrient. Results...
Hydrogen photoproduction by eukaryotic microalgae results from a connection between the photosynthetic electron transport chain and plastidial hydrogenase. Algal H2 production is transitory phenomenon under most natural conditions, often viewed as safety valve protecting overreduction. From colony screening of an insertion mutant library unicellular green alga Chlamydomonas reinhardtii based on analysis dark-light chlorophyll fluorescence transients, we isolated impaired in cyclic flow...
Calcineurin B-like proteins (CBLs) represent a family of calcium sensor that interact with group serine/threonine kinases designated as CBL-interacting protein (CIPKs). CBL-CIPK complexes are crucially involved in relaying plant responses to many environmental signals and regulating ion fluxes. However, the biochemical characterization has so far been hampered by low activities recombinant CIPKs. Here, we report on an efficient wheat germ extract-based vitro transcription/translation...
The basic question addressed in this study is how energy metabolism adjusted to cope with iron deficiency Chlamydomonas reinhardtii. To investigate the impact of on bioenergetic pathways, comparative proteomics was combined spectroscopic as well voltametric oxygen measurements assess protein dynamics linked functional properties respiratory and photosynthetic machineries. Although electron transfer largely compromised under deficiency, our quantitative data revealed that antenna size...
Cyclic photosynthetic electron flow (CEF) is crucial to photosynthesis because it participates in the control of chloroplast energy and redox metabolism, particularly induced under adverse environmental conditions. Here we report that down-regulation localized Ca 2+ sensor (CAS) protein by an RNAi approach Chlamydomonas reinhardtii results strong inhibition CEF anoxia. Importantly, this rescued increase extracellular concentration, inferring -dependent. Furthermore, identified a protein,...
* Guard cell movements are regulated by environmental cues including, for example, elevations in extracellular Ca(2+) concentration. Here, the subcellular localization and physiological function of Ca(2+)-sensing receptor (CAS) protein was investigated. CAS ascertained microscopic analyses green fluorescent (GFP) fusion proteins biochemical fractionation assays. Comparative guard movement investigations were performed wild-type cas loss-of-function mutant lines Arabidopsis thaliana....
Abstract The plant-specific calcium binding protein CAS (calcium sensor) has been localized in chloroplast thylakoid membranes of vascular plants and green algae. To elucidate the function Chlamydomonas reinhardtii, we generated analyzed eight independent knockdown C. reinhardtii lines (cas-kd). Upon transfer to high-light (HL) growth conditions, cas-kd were unable properly induce expression LHCSR3 that is crucial for nonphotochemical quenching. Prolonged exposure HL revealed a severe light...
Abstract The Chlamydomonas reinhardtii proton gradient regulation5 (Crpgr5) mutant shows phenotypic and functional traits similar to mutants in the Arabidopsis (Arabidopsis thaliana) ortholog, Atpgr5, providing strong evidence for conservation of PGR5-mediated cyclic electron flow (CEF). Comparing Crpgr5 with wild type, we discriminate two pathways CEF determine their maximum rates. PGR5/proton regulation-like1 (PGRL1) ferredoxin (Fd) pathway, involved recycling excess reductant increase ATP...
Summary RBOHF from Arabidopsis thaliana represents a multifunctional NADPH oxidase regulating biotic and abiotic stress tolerance, developmental processes guard cell aperture. The molecular components mechanisms determining activity remain to be elucidated. Here we combined protein interaction studies, biochemical genetic approaches, pathway reconstitution analyses identify characterize proteins that confer regulation elucidated adjust activity. While the Ca 2+ sensor‐activated kinases CIPK...
Abstract Photosystem I (PSI) enables photo-electron transfer and regulates photosynthesis in the bioenergetic membranes of cyanobacteria chloroplasts. Being a multi-subunit complex, its macromolecular organization affects dynamics photosynthetic membranes. Here we reveal chloroplast PSI from green alga Chlamydomonas reinhardtii that is organized as homodimer, comprising 40 protein subunits with 118 transmembrane helices provide scaffold for 568 pigments. Cryogenic electron microscopy...
Abstract Photosynthetic algae have evolved mechanisms to cope with suboptimal light and CO 2 conditions. When energy exceeds fixation capacity, Chlamydomonas reinhardtii activates photoprotection, mediated by LHCSR1/3 PSBS, the Concentrating Mechanism (CCM). How signals converge regulate these processes remains unclear. Here, we show that excess photoprotection- CCM-related genes altering intracellular concentrations depletion of drives responses, even in total darkness. High levels, derived...
ABSTRACT With the recent development of techniques for analyzing transmembrane thylakoid proteins by two-dimensional gel electrophoresis, systematic approaches proteomic analyses membrane became feasible. In this study, we established detailed protein maps Chlamydomonas reinhardtii light-harvesting (Lhca and Lhcb) extensive tandem mass spectrometric analysis. We predicted eight distinct Lhcb proteins. Although major were highly similar, identified peptides which unique specific lhcbm gene...
Summary Functional proteomics of membrane proteins is an important tool for the understanding protein networks in biological membranes but structural studies on this part proteome are limited. In study we undertook such approach to analyse photosynthetic thylakoid isolated from wild‐type and mutant strains Chlamydomonas reinhardtii. Thylakoid were separated by high‐resolution two‐dimensional gel electrophoresis (2‐DE) analysed immuno‐blotting mass spectrometry presence membrane‐spanning...
Photosynthetic organisms respond to changes in ambient light by modulating the size and composition of their light-harvesting complexes, which case green alga Chlamydomonas reinhardtii consists >15 members a large extended family chlorophyll binding subunits. How expression is coordinated unclear. Here, we describe analysis an insertion mutant, state transitions mutant3 (stm3), show has increased levels LHCBM subunits associated with antenna photosystem II. The mutated nuclear gene stm3...