A5100691215- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Immune Cell Function and Interaction
- Carbohydrate Chemistry and Synthesis
- T-cell and B-cell Immunology
- Berberine and alkaloids research
- Semiconductor materials and devices
- Cancer, Lipids, and Metabolism
- Electrochemical Analysis and Applications
- Glycosylation and Glycoproteins Research
- Ion-surface interactions and analysis
- Lipid metabolism and biosynthesis
- Pharmacogenetics and Drug Metabolism
- Alkaloids: synthesis and pharmacology
- Pesticide Residue Analysis and Safety
- Language, Metaphor, and Cognition
- Forensic Toxicology and Drug Analysis
- Analytical Chemistry and Sensors
- Antibiotics Pharmacokinetics and Efficacy
- Thin-Film Transistor Technologies
- Advancements in Semiconductor Devices and Circuit Design
Tsinghua University
2012-2025
Northeast Agricultural University
2025
Chinese Academy of Fishery Sciences
2025
Research Institute of Precision Instruments (Russia)
2025
Xidian University
2024
South China Agricultural University
2024
Qingdao Center of Resource Chemistry and New Materials
2024
China Southern Power Grid (China)
2024
Beijing University of Technology
2024
Xi'an Technological University
2009-2023
Mass spectrometry-based lipidomics is the primary tool for structural analysis of lipids but effective localization carbon-carbon double bonds (C=C) in unsaturated to distinguish C=C location isomers remains challenging. Here, we develop a large-scale lipid platform by coupling online derivatization through Paternò-Büchi reaction with liquid chromatography-tandem mass spectrometry. This provides rich information on isomers, revealing locations more than 200 glycerophospholipids bovine liver...
Lipids play a pivotal role in biological processes and lipid analysis by mass spectrometry (MS) has significantly advanced lipidomic studies. While the structure specificity of proves to be critical for studying functions lipids, current mainstream methods large-scale can only identify classes fatty acyl chains, leaving C=C location sn-position unidentified. In this study, combining photochemistry tandem MS we develop simple but effective workflow enable near-complete characterization with...
Abstract Single-cell analysis is critical to revealing cell-to-cell heterogeneity that would otherwise be lost in ensemble analysis. Detailed lipidome characterization for single cells still far from mature, especially when considering the highly complex structural diversity of lipids and limited sample amounts available a cell. We report development general strategy enabling single-cell lipidomic with high specificity. Cell fixation applied retain cell during batch treatments prior In...
Small molecules that can selectively target cancer stem cells (CSCs) remain rare currently and exhibit no common structural features. Here we report a series of guaianolide sesquiterpene lactones (GSLs) their derivatives eradicate acute myelogenous leukemia (AML) or progenitor cells. Natural GSL compounds arglabin, an anticancer clinical drug, micheliolide (MCL), are able to reduce the proportion AML (CD34⁺CD38⁻) in primary Targeting is further confirmed by sharp reduction colony-forming...
Mass spectrometry (MS) imaging of lipids in tissues with high structure specificity is challenging the effective fragmentation position-selective structures and sensitive detection multiple lipid isomers. Herein, we develop an MS3 method for simultaneous analysis phospholipid C═C sn-position isomers by on-tissue photochemical derivatization, nanospray desorption electrospray ionization (nano-DESI), a dual-linear ion trap MS system. A novel laser-based sensing probe developed real-time...
This study aimed to assess the impact of incorporating rapeseed meal (RM) as a partial substitute for fish (FM) in diet cultured hybrid sturgeon ( Acipenser schrenckii ♀ × baerii ♂). A total 450 juvenile with similar weights were randomly assigned five dietary groups, each triplicates 30 per tank. For 12 weeks, FM was replaced varying percentages RM (0%, 25%, 50%, 75%, and 100%). Results indicated decreasing trend final body weight (FBW), gain, survival rates (SRs) ratio increased. Growth...
Introducing structural modifications into biomolecules represents a powerful approach to dissect their functions and roles in biological processes. Bacterial polysaccharides, despite rich information essential bacterium-host interactions bacterial virulence, have largely been unexplored for vivo modifications. In this study, we demonstrate the incorporation of panel monosaccharide analogs polysaccharides highly homogenous manner via metabolic engineering promiscuous sugar nucleotide...
MicroRNAs (miRNAs) have emerged as critical epigenetic regulators involved in cancer progression. miR-320a has been identified to be a novel tumour suppressive miRNA colorectal (CRC). However, the detailed molecular mechanisms are not fully understood. Here, we reported that inversely associated with CRC aggressiveness both cell lines and clinical specimens. Functional studies demonstrated significantly decreased capability of migration/invasion induced G0/G1 growth arrest vitro vivo....
Graphene is a new kind of two-dimensional carbon nanomaterial with excellent properties and promising for solid-phase microextraction (SPME). Plastic microtubes such as poly(tetrafluoroethylene) (PTFE) poly(ether ether ketone) are ideal substrates in-tube SPME. However, immobilization graphene layers onto these materials still problem due to their nature chemical resistance. In order solve the problem, we proposed novel method based on universal mussel-inspired polydopamine (PD)...
MicroRNAs (miRNAs) have been reported to play crucial roles in regulating a variety of genes pivotal for tumor metastasis. MicroRNA-301a (miR-301a) is overexpressed and displays oncogenic activity many cancers. However, little known about the potential miR-301a colorectal cancer (CRC). Taqman probe stem-loop real-time PCR was used quantitatively measure expression level 48 cases CRC tissues matched adjacent non-tumor mucosa as well cell lines. mimics inhibitors were up-regulate down-regulate...
As a major class of mammalian lipids, phosphatidylcholines (PCs) often contain mixtures structural isomers, resulting from different lipogenesis pathways. Profiling PCs at the isomer level, however, remains challenging in lipidomic settings, especially for characterizing positions fatty acyls on glycerol backbone (sn-positions) and locations carbon-carbon double bonds (C[double bond, length as m-dash]Cs) unsaturated acyl chains. In this work, we have developed workflow profiling down to sn-...
Currently there are no effective therapies available for the excruciating neuropathic pain that develops after spinal cord injuries (SCI). As such, a great deal of effort is being put into investigation novel therapeutic targets can alleviate this pain. One such target acrolein, highly reactive aldehyde produced as byproduct oxidative stress and inflammation capable activating transient receptor potential ankyrin 1 (TRPA1) cation channel, known to be involved in transmission propagation...
The development and performance of two mass spectrometry (MS) workflows for the intraoperative diagnosis isocitrate dehydrogenase (IDH) mutations in glioma is implemented by independent teams at Mayo Clinic, Jacksonville, Huashan Hospital, Shanghai. infiltrative nature gliomas makes rapid necessary to guide extent surgical resection central nervous system (CNS) tumors. combination tissue biopsy MS analysis used here satisfies this requirement. key feature both described methods use tandem...