A5031283652- Spine and Intervertebral Disc Pathology
- Cervical and Thoracic Myelopathy
- Spinal Fractures and Fixation Techniques
- Musculoskeletal pain and rehabilitation
- Medical Imaging and Analysis
- Pain Mechanisms and Treatments
- Scoliosis diagnosis and treatment
- Spinal Cord Injury Research
- Spinal Hematomas and Complications
- Spondyloarthritis Studies and Treatments
- Bone Metabolism and Diseases
- Peripheral Artery Disease Management
- Infectious Diseases and Tuberculosis
- Anesthesia and Pain Management
- Pharmacological Effects of Natural Compounds
- Pelvic and Acetabular Injuries
- Plant Toxicity and Pharmacological Properties
- Vascular Procedures and Complications
- Venous Thromboembolism Diagnosis and Management
- TGF-β signaling in diseases
- Heterotopic Ossification and Related Conditions
- Hippo pathway signaling and YAP/TAZ
- Cancer Treatment and Pharmacology
- Glutathione Transferases and Polymorphisms
- Shoulder Injury and Treatment
Shanghai Changzheng Hospital
2015-2024
Central South University
2020-2023
State Key Laboratory of High Performance Complex Manufacturing
2023
Ruijin Hospital
2013-2022
Second Military Medical University
2013-2022
Shanghai Jiao Tong University
2008-2021
Affiliated Hospital of Guizhou Medical University
2019-2021
Xiangya Hospital Central South University
2020
Traditional Chinese Medicine Hospital of Kunshan
2019
Shanghai University of Traditional Chinese Medicine
2019
Evidence suggests that intervertebral disc degeneration (IVDD) can be induced by Propionibacterium acnes (P. acnes), although the underlying mechanisms are unclear. In this study, we analyzed pathological changes in degenerated human discs (IVDs) infected with P. acnes. Compared acnes-negative samples, acnes-positive IVDs showed increased apoptosis of nucleus pulposus cells (NPCs) concomitant severe IVDD. Then, a acnes-inoculated IVD animal model was established, and IVDD infection promoting...
The precise role of apoptosis in the pathogenesis intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that expression miR-27a was increased. overexpression further verified using real-time RT-PCR. Bioinformatics target prediction identified phosphoinositide-3 kinases (PI3K) as putative targets miR-27a. Furthermore, inhibited PI3K by directly targeting their 3'-UTRs, this inhibition abolished mutation binding sites....
Abstract Objectives Apoptosis of nucleus pulposus ( NP ) cells is a major cause intervertebral disc degeneration. To elucidate relationships between caveolin‐1 and cytokine‐induced apoptosis, we investigated the role in apoptosis rat related signalling pathway. Materials methods Rat were treated with interleukin IL )‐1β or tumour necrosis factor alpha TNF ‐α), knockdown β‐catenin was achieved using specific si RNA s. Then, apoptotic level expression activation caveolin‐1/β‐catenin assessed...
Currently there are no effective therapies available for the excruciating neuropathic pain that develops after spinal cord injuries (SCI). As such, a great deal of effort is being put into investigation novel therapeutic targets can alleviate this pain. One such target acrolein, highly reactive aldehyde produced as byproduct oxidative stress and inflammation capable activating transient receptor potential ankyrin 1 (TRPA1) cation channel, known to be involved in transmission propagation...
Abstract P38MAPK mediates cytokine induced inflammation in nucleus pulposus (NP) cells and involves multiple cellular processes which are related to intervertebral disc degeneration (IDD). The aim of this study was investigate the expression, activation function p38 MAPK isoforms (α,β, γ δ) degenerative NP effect on macrophage polarization. P38 α, β δ preferential expressed, whereas p38γ isoform is absent human tissue. LV-sh-p38α, sh-p38β transfection significantly decreased ADAMTS-4,-5,...
Purpose . To investigate whether P. acnes could induce disc degeneration and Modic changes when inoculated into the discs of rabbits. Method A wild-type strain isolated from a patient associated with change was intervertebral Meanwhile, S. aureus injected to establish model discitis as comparison standard control. MRI histological were observed. Results Both acnes- -inoculated rabbits showed hyperintense signals at endplates hypointense nucleus pulposus on T2WI. However, only resulted in...
Abstract Four-octyl itaconate (4-OI) is the cell-permeable derivative of that can activate Nrf2 signaling by alkylating Keap1’s cysteine residues. Here, we tested potential effect 4-OI on hydrogen peroxide (H 2 O )-induced oxidative injury in osteoblasts. In OB-6 cells and primary murine osteoblasts, was able to cascade cause Keap1–Nrf2 disassociation, protein stabilization, cytosol accumulation, nuclear translocation. also augmented antioxidant-response element reporter activity promoted...
Abstract Sensory nerves are long being recognized as collecting units of various outer stimuli; recent advances indicate that the sensory nerve also plays pivotal roles in maintaining organ homeostasis. Here, this study shows orchestrates intervertebral disc (IVD) homeostasis by regulating its extracellular matrix (ECM) metabolism. Specifically, genetical denervation IVD results loss water preserve molecule chondroitin sulfate (CS), reduction CS bio‐synthesis gene synthase 1 (CHSY1)...
Abstract Acrolein, an endogenous aldehyde, has been shown to be involved in sensory hypersensitivity after rat spinal cord injury ( SCI ), for which the pathogenesis is unclear. Acrolein can directly activate a pro‐algesic transient receptor protein ankyrin 1 TRPA 1) channel that exists neurons. Both acrolein and mRNA are elevated post , contributes activation of by consequently, neuropathic pain. In current study, we further showed that, post‐ elevation not only dorsal root ganglias but...
Accumulating evidence suggests that Propionibacterium acnes (P. acnes) is a novel pathogenic factor promoting intervertebral disc degeneration (IVDD). However, the underlying mechanisms by which P. induces IVDD have been unclear. In this study, we quantified severity of IVDD, as well expressions inducible nitric oxide synthase (iNOS)/nitric (NO) and cyclooxygenase (COX-2)/prostaglandin (PGE2) in human discs (IVDs) infected with acnes. Compared acnes-negative IVDs, acnes-positive IVDs showed...
Background contextLaminoplasty is a common surgical method used to treat patients with cervical ossification of the posterior longitudinal ligament (OPLL). Although laminoplasty preferred over traditional laminectomy, factors affecting complications and outcomes are unclear. Recently, sagittal balance indexes have been revealed be predictors clinical in degenerative diseases, but their relationships laminoplasty-treated OPLL remains unknown.PurposeThe purpose this study evaluate relationship...
Abstract An ultra-large structure-based virtual screening has discovered iKeap1 as a direct Keap1 inhibitor that can efficiently activate Nrf2 signaling. We here tested its potential effect against hydrogen peroxide (H 2 O )-induced oxidative injury in osteoblasts. In primary murine and human osteoblasts, robustly activated signaling at micromole concentrations. disrupted Keap1-Nrf2 association, causing protein stabilization, cytosol accumulation nuclear translocation The anti-oxidant...
A three-dimensional (3D) display provides an immersive user experience and represents the frontier of technology. The 3D based on polarizer films is relatively mature. However, currently used lead to bulk systems low efficiency. Micro-light-emitting diodes (micro-LEDs) have advantages small size, high contrast, power consumption, response frequency. micro-LED displays combine characteristics micro-LEDs simultaneously, which can be in portable electronics or projectors users with experience....
Study Design: A retrospective study. Objective: To compare clinical and radiologic outcomes of 3-level anterior cervical discectomy fusion between a zero-profile (Zero-P) spacer traditional plate in cases symptomatic spine spondylosis. Summary Background Data: Anterior decompression is indicated for patients with compression or stenosis the spinal cord. The Zero-P spacers have been used interbody 1 2 segments. However, there paucity published data regarding exact impact device on curvature...
Objective To investigate the clinical and imaging predictors of surgical outcomes in patients with ossification posterior longitudinal ligament (OPLL). Materials Methods From May 2010 to April 2012, a total 200 consecutive cervical OPLL were recruited for this study. Of them, 184 (130 men 54 women) who could be tracked more than 24 months after surgery finally included analysis. Their demographic, radiological data collected preoperatively. The recovery ratio terms JOA score was used assess...