A5101576440- Spine and Intervertebral Disc Pathology
- Cervical and Thoracic Myelopathy
- Musculoskeletal pain and rehabilitation
- Spinal Fractures and Fixation Techniques
- Medical Imaging and Analysis
- Spinal Hematomas and Complications
- Osteoarthritis Treatment and Mechanisms
- Bone Metabolism and Diseases
- Pain Mechanisms and Treatments
- Plant Toxicity and Pharmacological Properties
- Inflammatory mediators and NSAID effects
- Scoliosis diagnosis and treatment
- TGF-β signaling in diseases
- Proteoglycans and glycosaminoglycans research
- Hippo pathway signaling and YAP/TAZ
- Anesthesia and Pain Management
- Extracellular vesicles in disease
- Pregnancy-related medical research
- Studies on Chitinases and Chitosanases
- Endometriosis Research and Treatment
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Tendon Structure and Treatment
- Advanced Memory and Neural Computing
- Rheumatoid Arthritis Research and Therapies
- Retinoids in leukemia and cellular processes
Shanghai Changzheng Hospital
2014-2025
Ankara Spine Center
2023
Second Military Medical University
2009-2022
Whether sensory nerve can sense bone density or metabolic activity to control homeostasis is unknown. Here we found prostaglandin E2 (PGE2) secreted by osteoblastic cells activates PGE2 receptor 4 (EP4) in nerves regulate formation inhibiting sympathetic through the central nervous system. osteoblasts increases when decreases as demonstrated osteoporotic animal models. Ablation of erodes skeletal integrity. Specifically, knockout EP4 gene cyclooxygenase-2 (COX2) significantly reduces volume...
Abstract Intervertebral disc (IVD) degeneration is known to aggravate with age and oxidative stress implicated in the pathogenesis of many age-related diseases. Nuclear factor (erythroid-derived-2)-like 2 (Nrf2) can confer adaptive protection against proteotoxic cells. In this study, we assessed whether Nrf2 protect nucleus pulposus (NP) addition, investigated expression NP tissue samples from patients different degrees IVD a mouse model aging influence H O -induced on autophagic pathways...
The sensory nerve was recently identified as being involved in regulation of bone mass accrual. We previously discovered that prostaglandin E2 (PGE2) secreted by osteoblasts could activate EP4 receptor to promote formation inhibiting sympathetic activity. However, the fundamental units are active osteoblasts, which originate from mesenchymal stromal/stem cells (MSCs). Here, we found after denervation, knockout nerves, or COX-2 significantly adipogenesis and inhibit osteogenesis adult mice....
The precise role of apoptosis in the pathogenesis intervertebral disc degeneration (IDD) remains to be elucidated. We analyzed degenerative nucleus pulposus (NP) cells and found that expression miR-27a was increased. overexpression further verified using real-time RT-PCR. Bioinformatics target prediction identified phosphoinositide-3 kinases (PI3K) as putative targets miR-27a. Furthermore, inhibited PI3K by directly targeting their 3'-UTRs, this inhibition abolished mutation binding sites....
Abstract Objectives Apoptosis of nucleus pulposus ( NP ) cells is a major cause intervertebral disc degeneration. To elucidate relationships between caveolin‐1 and cytokine‐induced apoptosis, we investigated the role in apoptosis rat related signalling pathway. Materials methods Rat were treated with interleukin IL )‐1β or tumour necrosis factor alpha TNF ‐α), knockdown β‐catenin was achieved using specific si RNA s. Then, apoptotic level expression activation caveolin‐1/β‐catenin assessed...
Abstract Spinal cord injury (SCI) represents a profound central nervous system affliction, resulting in irreversibly compromised daily activities and disabilities. SCI involves excessive inflammatory responses, which are characterized by the existence of high levels proinflammatory M1 macrophages, neuronal mitochondrial energy deficit, exacerbating secondary damage impeding axon regeneration. This study delves into mechanistic intricacies SCI, offering insights from perspectives neuroimmune...
Background contextLaminoplasty is a common surgical method used to treat patients with cervical ossification of the posterior longitudinal ligament (OPLL). Although laminoplasty preferred over traditional laminectomy, factors affecting complications and outcomes are unclear. Recently, sagittal balance indexes have been revealed be predictors clinical in degenerative diseases, but their relationships laminoplasty-treated OPLL remains unknown.PurposeThe purpose this study evaluate relationship...
Prospective clinical study.To identify changes in depression and anxiety after cervical total disc replacement (CTDR) among patients with spondylosis, to investigate their effects on the prognosis.Previous researches have reported relationships between mood disorders lumbar surgery. There been no previous studies of depressive CTDR outcome at 2-year postoperative phase.Eighty-five spondylosis who underwent were included. Patients evaluated preoperatively 6 time points, including 1 week,...
Intervertebral discs (IVDs) provide stability and flexibility to the spinal column; however, IVDs, in particular nucleus pulposus (NP), undergo a degenerative process characterized by changes disc extracellular matrix (ECM), decreased cell viability, reduced synthesis of proteoglycan type II collagen. Here, we investigated efficacy feasibility stem therapy using bone marrow mesenchymal cells (BMSCs) over-expressing morphogenetic protein 7 (BMP7) promote ECM remodeling degenerated IVDs....
Abstract Intervertebral disc degeneration (IDD) is characterized by disordered extracellular matrix (ECM) metabolism, implicating subdued anabolism and enhanced catabolic activities in the nucleus pulposus (NP) of discs. Pro-inflammatory cytokines such as interleukin-1β (IL-1β) are considered to be potent mediators ECM breakdown. Hemeoxygenase-1 (HO-1) has been reported participate cellular anti-inflammatory processes. The purpose this study was investigate HO-1 modulation metabolism human...
Intervertebral disc degeneration (IDD) has been identified as one of the predominant factors leading to persistent low back pain and disability in middle-aged elderly people. Dysregulation Prostaglandin E2 (PGE2) can cause IDD, while low-dose celecoxib maintain PGE2 at physiological level activate skeletal interoception. Here, nano fibers have extensively used treatment novel polycaprolactone (PCL) loaded with were fabricated for IDD treatment. In vitro studies demonstrated that had ability...
ABSTRACT The enzyme chondroitin polymerizing factor (ChPF) is primarily involved in extension of the sulfate backbone required for synthesis sulfated glycosaminoglycan (sGAG). Transforming growth beta (TGF‐β) upregulates sGAG nucleus pulposus cells; however, mechanisms mediating this induction are incompletely understood. Our study demonstrated that ChPF expression was negatively correlated with grade degenerative intervertebral disc disease. Treatment cells TGF‐β induced and enhanced...
// Bo Hu 1, * , Chen Xu Ye Tian Changgui Shi 1 Ying Zhang Lianfu Deng 2 Hongyu Zhou Peng Cao Huajiang and Wen Yuan Department of Spine Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, China Shanghai Key Laboratory for Bone Joint Diseases, Institute Orthopaedics Traumatology, Ruijin Jiao Tong University School Medicine, These authors contributed equally to this work Correspondence to: Chen, email: spine.chen@163.com Yuan, yuanwenspine@126.com Cao,...
The interspinous process motion (ISM) method can provide a more accurate assessment of postoperative subaxial cervical fusion than Cobb angle which is used commonly in clinical practice. However, the ISM presents measurement results millimeters cannot be directly compared with data. We proposed modified for functional spinal unit range (FSU ROM) and evaluate its repeatability reliability measuring ROM surgical segment after artificial disc replacement surgery. A total 81 patients who...
Study Design Retrospective Cohort Study. Objective The aim of this study was to evaluate the efficacy our proposed surgical procedure named unilateral exposure channel-assisted (UCA) laminoplasty in treating degenerative cervical myelopathy (DCM), and postoperative volume changes paraspinal muscles. Methods From January 2020 2022, 104 patients with DCM underwent open-door laminoplasty, among which, 63 UCA (UCA group) 41 traditional (LP group). radiological clinical parameters as well...
Background/Aims: Intervertebral discs consist of an extracellular matrix (ECM) with a central gelatinous nucleus pulposus (NP) enclosed in outer layer known as the annulus fibrosus. ECM metabolic disorders result loss boundary between fibrosus and NP, which can lead to intervertebral disc degeneration (IDD). Proinflammatory cytokines, such interleukin (IL)-1β, mediate progression IDD. Nicotinamide phosphoribosyltransferase (Nampt) catalyzes first step biosynthesis nicotinamide adenine...
Background/Aims: Chondroitin sulfate synthase 1 (CHSY1) is a glycosyltransferases involved in the biosynthesis of chondroitin and dermatan glycosaminoglycan (GAG). TGF-β can stimulate sulfated GAG production nucleus pulposus cells; however, underlying mechanisms are poorly understood. Methods: CHSY1 expression was examined rat treated with using real-time PCR Western blot analysis. Lentiviral knockdown performed to determine downstream effectors measure effect c-Jun Sp1 on mediated promoter...
Degeneration of intervertebral disc is a major cause lower back pain and neck pain. Studies have tried to unveil the regulatory network using either transcriptomic or proteomic analysis. However, neither fully elucidated exact mechanism degeneration process. Since post-transcriptional regulation may affect gene expression by modulating translational process mRNA protein product, combined study provide more insight into key Intervertebral degeneration. In order obtain data, we performed...
Four-level cervical spondylotic myelopathy (CSM) is a common disease affecting large number of people, with the optimal surgical strategy remaining controversial. This study compared clinical outcomes, radiological parameters, and postoperative complications primarily performed procedures such as anterior discectomy fusion (ACDF), open-door laminoplasty (LAMP), laminectomy (LF) in treating four-level CSM. A total 116 patients who received ACDF (38 cases), LAMP (45 LF (33 cases) were followed...