The medical community has expressed significant interest in the development of new types artificial bones that mimic natural bones. In this study, computed tomography (CT)-guided fused deposition modeling (FDM) was employed to fabricate polycaprolactone (PCL)/hydroxyapatite (HA) and PCL 3D goat femurs. vitro mechanical properties, cell biocompatibility, vivo performance a long load-bearing femur bone segmental defect model were studied. All results indicate CT-guided FDM is simple,...

Diabetic retinopathy (DR) is a well-known microvascular complication related to inflammation. Mcc950 potent and specific inhibitor of the NLRP3 inflammasome but its influence on DR has not been studied. Thus, we evaluated anti-inflammatory effects high-glucose-induced human retinal endothelial cells (HRECs) potential underlying mechanism. In surgical excised proliferative membranes from patients, high expression NLRP3, caspase 1 IL-1β was observed co-localization occurred in CD31+ labeled...

Abstract During peripheral nerve regeneration, current deoxyribonucleic acid (DNA)‐based therapeutic platforms face the challenge of precisely regulating Schwann cells (SCs) fate to sustain their repair phenotype due inability stably and integrate multiple bioactive components. Herein, strain‐promoted azide–alkyne cycloaddition reaction is utilized neurotrophic factor mimetic peptide RGI laminin‐derived IKVAV into DNA monomers. Through sequence self‐assembly, a programmable DNA‐peptide...

Abstract Surface-modified metal implants incorporating different ions have been employed in the biomedical field as bioactive dental with good osseointegration properties. However, molecular mechanism through which surface coatings exert biological activity is not fully understood, and effects difficult to achieve, especially osteopenic bone. In this study, We examined effect of zinc-modified calcium silicate two Zn contents induce osteogenic differentiation rat bone marrow-derived pericytes...

Lateral lumbar interbody fusion (LLIF) is a popular, minimally invasive technique that used to address challenging multilevel degenerative spinal diseases. It remains controversial whether supplemental instrumentation should be added for LLIF. In this study, we compared the kinematic stability afforded by stand-alone lateral cages with those supplemented bilateral pedicle screws and rods (PSR), unilateral PSR, or plate (LP) fixation using finite-element (FE) model of multi-level LLIF...

Introduction: Curcumin (CUR) is a general ingredient of traditional Chinese medicine, which has potential antitumor effects. However, its use clinically been limited due to low aqueous solubility and bioavailability. In order improve the therapeutic effect CUR on osteosarcoma (i.e., bone cancer), multifunctional micelle was developed here by combining active accumulating ability with tumor CD44 targeting capacity. Methods: The loaded micelles were self-assembled using alendronate-hyaluronic...

Abstract Background To compare standalone oblique lateral interbody fusion (OLIF) vs. OLIF combined with posterior bilateral percutaneous pedicle screw fixation (OLIF combined) for the treatment of lumbar spondylolisthesis. Methods This was a retrospective study patients who underwent or between 07/2014 and 08/2017 at two hospitals in China. Direct decompressions were not performed. Visual analog scale (VAS), Oswestry Disability Index (ODI), satisfaction rate, anterior/posterior disc heights...

Post-surgical peritoneal adhesions constitute a classic problem in surgery, and thus anti-adhesion materials are much required. In this study, series of polyester-PEG-polyester triblock copolymers with different biodegradable polyester compositions were synthesized, their properties examined, the vivo efficacies as biomaterials evaluated comparative way for first time. These samples not only exhibited various morphologies bulk state, but also possessed stabilities sol state. All polymer...

The effective treatment of urethral stricture remains a medical problem. use proinflammatory cytokines as stimuli to improve the reparative efficacy mesenchymal stem cells (MSCs) towards damaged tissues represents an evolving field investigation. However, therapeutic benefits this strategy in remain unknown. Here, we enriched exosomes derived from human umbilical cord-derived MSCs pretreated with or without tumor necrosis factor alpha (TNF-α) evaluate their effects vivo model TGFβ1-induced...

A novel electrospun-aligned nanoyarn/three-dimensional porous nanofibrous hybrid scaffold for annulus fibrosus tissue engineering Jun Ma,1,* Yunfei He,1,2,* Xilin Liu,1,* Weiming Chen,3 An Wang,1,4 Chia-Ying Lin,5,6 Xiumei Mo,3 Xiaojian Ye1 1Department of Orthopaedics, Changzheng Hospital, Second Military Medical University, Shanghai, 2Department Spinal Surgery, Lanzhou General Hospital Command Region, Lanzhou, 3College Chemistry, Chemical Engineering and Biotechnology, Donghua 4Department...

Although mycobacterial proteins in exosomes from peripheral serum of patients with tuberculosis (TB) have been identified, other exact compositions remain unknown. In the present study, a comprehensive proteomics analysis derived active TB (ATB) was performed. Exosomes ATB were characterized using nanoparticle tracking (NTA), transmission electron microscopy (TEM), and western blotting analysis. Then identified protein components quantified by label-free determined via bioinformatics A total...

Objective . Special hypoxic and hypertonic microenvironment in intervertebral discs (IVDs) decreases the treatment effect of cell transplantation. We investigated hypothesis that preconditioning (HP) could improve therapeutic bone mesenchymal stem cells (BMSCs) to IVD degeneration. Methods. BMSCs from green fluorescent protein-transgenic rats were pretreated with cobalt chloride (CoCl 2 , 100 μ M, 24 h) for conditions vitro Apoptosis (related pathways caspase-3 bcl-2) migration HIF-1 α...

There is no available literature for comparison on muscle atrophy between the "stand-alone" oblique lateral interbody fusion (OLIF) and regular OLIF (i.e., combined with percutaneous pedicle screws fixation (PPSF) in patients spondylolisthesis). This study aimed to identify changes back two surgeries.This was a retrospective cohort of who underwent or OLIF+PPSF at Beijing Jishuitan Hospital Shanghai ChangZheng 07/2014 10/2017. Computed tomography (CT) used measure functional cross-sectional...

Osteosarcoma (OS) is a malignant bone tumor among adolescents and young adults. IRF7 belongs to the transcription factor family of interferon regulatory factors (IRFs) has previously been described function as suppressor in multiple cancer types. However, biological functions cellular mechanism OS remain elusive. In this study, by quantitative real-time PCR (qRT-PCR) western blotting, we found that was downregulated OS, higher expression correlated with better survival prognosis. Moreover,...

Osteoporosis is a common disease among the ageing society. Oxidative stress caused by excessive accumulation of reactive oxygen species (ROS) aetiology osteoporosis. α-Lipoic acid (ALA) an antioxidant in body, which can eliminate excess ROS body and inhibits levels oxidative cells. Herein, we designed PEGylated hollow gold nanoparticles (HGNPs) loaded with ALA (mPEG@HGNPs-ALA) to remove treatment First, mPEG@HGNPs particle size ∼63 nm has been successfully synthesized. By comparing drug...

An apatite/wollastonite-derived (A/W) porous glass ceramic scaffold with highly interconnected pores was successfully fabricated by adding a plastic porosifier. The morphology, porosity and mechanical strength were characterized. results showed that the controllable pore size displayed open macropores. In addition, good in vitro bioactivity found for obtained soaking it simulated body fluid. Mesenchymal stem cells (MSCs) cultured, expanded seeded on scaffold, adhesion proliferation of MSCs...