A5101433491- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Intraocular Surgery and Lenses
- Retinal and Macular Surgery
- Retinal Development and Disorders
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Extracellular vesicles in disease
- Ocular Diseases and Behçet’s Syndrome
- Neuroinflammation and Neurodegeneration Mechanisms
- Chronic Kidney Disease and Diabetes
- Drug Transport and Resistance Mechanisms
- Corneal surgery and disorders
- Cytokine Signaling Pathways and Interactions
- Vascular Malformations Diagnosis and Treatment
- Sphingolipid Metabolism and Signaling
- Cholesterol and Lipid Metabolism
- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Immune Response and Inflammation
- interferon and immune responses
- Carbohydrate Chemistry and Synthesis
- Ophthalmology and Visual Impairment Studies
- Glycosylation and Glycoproteins Research
- Airway Management and Intubation Techniques
Nanjing Medical University
2016-2025
Jiangsu Province Hospital
2016-2025
Jiujiang First People's Hospital
2012-2024
Huzhou University
2024
Huzhou Central Hospital
2017-2024
Zhejiang University
2024
Jiamusi University
2024
81th Hospital of PLA
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2023
Weatherford College
2023
M2-polarized tumor-associated macrophages (TAMs) are key regulators of the link between inflammation and cancer. A negative correlation infiltration intensity TAMs prognosis pancreatic cancer has been reported. Epithelial-mesenchymal transition (EMT) is an important biological process in progression primary tumors toward metastasis. Inflammation-induced EMT previously shown, therefore, we hypothesized could induce Toll-like receptor 4 (TLR4) signaling active role tumor during chronic...
Klotho , an antiaging gene with restricted organ distribution, is mainly expressed in the kidney tubules; mutant mice have shortened life span, arteriosclerosis, anemia, and osteoporesis, features common to patients chronic renal failure. Conceivably, reduction of expression may contribute development failure; alternatively, its overexpression lead amelioration injury ICR-derived glomerulonephritis (ICGN) mouse model subtle immune complex-mediated disease. To address this issue, four...
Diabetic retinopathy (DR) is a well-known microvascular complication related to inflammation. Mcc950 potent and specific inhibitor of the NLRP3 inflammasome but its influence on DR has not been studied. Thus, we evaluated anti-inflammatory effects high-glucose-induced human retinal endothelial cells (HRECs) potential underlying mechanism. In surgical excised proliferative membranes from patients, high expression NLRP3, caspase 1 IL-1β was observed co-localization occurred in CD31+ labeled...
Age-related macular degeneration (AMD) is the leading cause of blindness among elderly in developed countries. Although pathogenic factors, such as oxidative stress, inflammation and genetics are thought to contribute development AMD, little known about relationships priorities between these factors. Here, we show that chronic photo-oxidative stress an environmental factor involved AMD pathogenesis. We first demonstrated exposure light induced phospholipid oxidation mouse retina, which was...
Abstract It is known that oxidative stress plays a pivotal role in age-related macular degeneration (AMD) pathogenesis. Alpha-mangostin the main xanthone purified from mangosteen as anti-oxidative properties. The aim of study was to test protective effect alpha-mangostin against both retina light-damaged mice model and hydrogen peroxide (H 2 O )-stressed RPE cells. We observed significantly inhibited light-induced photoreceptors 200 μM H -induced apoptosis generation reactive oxygen species...
Vitreous fibrovascular membranes (FVMs), the hallmark of proliferative diabetic retinopathy (PDR), cause retinal hemorrhage, detachment, and eventually blindness. However, little is known about pathophysiology FVM. In this study, we used single-cell RNA sequencing on surgically harvested PDR-FVMs generated a comprehensive cell atlas Eight cellular compositions were identified, with microglia as major population. We identified GPNMB+ subpopulation microglia, which presented both profibrotic...
Accumulating evidence has suggested the significant role of long noncoding RNAs (lncRNA) in regulating ferroptosis, while its regulatory mechanism diabetic retinopathy (DR) remains unelucidated. In this work, we first demonstrated that lncRNA zinc finger antisense 1 (ZFAS1) is upregulated high glucose-cultured human retinal endothelial cells (hRECs) and ZFAS1 inhibition attenuated glucose- (HG-) induced which was evidenced by cell viability, total iron ferrous levels, reactive oxygen species...
Abstract Diabetic retinopathy (DR) is a vision-threatening diabetic complication that characterized by microvasculature impairment and immune dysfunction. The present study demonstrated M2 microglia intensively participated in retinal microangiopathy human proliferative membranes, mice retinas, retinas of with oxygen-induced (OIR) mice, streptozotocin-induced DR mice. Further vivo vitro experiments showed exosomes derived from polarized (M2-exo) could reduce pericyte apoptosis promote...
Atrogin-1/MAFbx is a major atrophy-related E3 ubiquitin ligase that expressed specifically in striated muscle. Although the contribution of atrogin-1 to cardiac and muscle hypertrophy/atrophy has been examined extensively, it remains unclear whether plays an essential role simulated ischemia/reperfusion-induced apoptosis primary cardiomyocytes. Here we showed markedly enhanced cardiomyocytes via activation JNK signaling. Overexpression increased phosphorylation c-Jun decreased Foxo3a. In...
Diabetic retinopathy is a heterogeneous retinal degenerative disease with the microvascular dysfunction being recognized as hallmark of advanced stage. In this study, we demonstrated that exosomes collected from vitreous humor proliferative diabetic patients promoted proliferation, migration and tube formation ability primary human endothelial cells via its elevated miR-9-3p expression level. Müller glia were further sole source aberrantly expressed miR-9-3p, both in vitro vivo experiments...
Diabetic retinopathy (DR), one of the most common microangiopathic complications in diabetes, causes severe visual damage among working-age populations. Retinal vascular endothelial cells, key cell type DR pathogenesis, are responsible for abnormal retinal angiogenesis advanced stages DR. The roles exosomes have been largely unknown. In this study, we report first evidence that derived from vitreous humor patients with proliferative (PDR-exo) promote proliferation, migration, and tube...
Fritillaria ussuriensis is an endangered medicinal plant known for its notable therapeutic properties. Unfortunately, population has drastically declined due to the destruction of forest habitats. Thus, effectively protecting F. from extinction poses a significant challenge. A profound understanding genetic foundation crucial. To date, research on complete mitochondrial genome not yet been reported.
Niemann-Pick C1-Like 1 (NPC1L1) mediates intestinal absorption of dietary and biliary cholesterol. Ezetimibe, by inhibiting NPC1L1 function, is widely used to treat hypercholesterolemia in humans. Interestingly, ezetimibe treatment appears attenuate hepatic steatosis rodents humans without a defined mechanism. Overconsumption high-fat diet (HFD) represents major cause metabolic disorders including fatty liver. To determine whether how deficiency prevents HFD-induced steatosis, this study, we...
Purpose. To describe both the functional and pathological alternations in neurosensory retina a murine model of spontaneous type 2 diabetes (db/db mouse). Methods. db/db (BKS/DB-/-) mice heterozygous littermates (as control group) at various ages (12, 16, 20, 24, 28 weeks) were inspected with pattern electroretinogram (PERG), fundus fluorescein angiography (FFA), optical coherence tomography (OCT). Histological markers neuroinflammation (IBA-1 F4/80) evaluated by immunohistochemistry. In...
Diabetic retinopathy (DR) is one of the common and specific microvascular complications diabetes. This study aimed to investigate anti-angiogenic effect kaempferol explore its underlying molecular mechanisms. The mRNA expression level vascular endothelial growth factor (VEGF) placenta (PGF) concentrations secreted VEGF PGF were measured by qTR-PCR ELISA assay, respectively. Human retinal cells (HRECs) proliferation, migration, sprouting CCK-8 transwell, scratching wound, tube formation...
NLRP3, as a crucial inflammasome component, plays important roles in age-related macular degeneration. Though some activators of NLRP3 have been studied, microRNAs (miRNAs) which potentially regulate messenger RNA (mRNA) not fully explored retinal pigment epithelial (RPE) cells and retinopathy. In this study, by miRNA microarray profiling bioinformatic analysis, we identified that four miRNAs, miR-4286, miR-223-3p, miR-365a, miR-22-3p, may target mRNA RPE inflammatory damage vivo. Further,...
The role of tubular injury in diabetic nephropathy is relatively unknown, despite that apoptosis epithelial cells commonly observed human renal biopsies. GTPase Ras-proximate-1 (Rap1b) upregulated the hyperglycemic state and known to increase B-Raf, an antiapoptotic effector protein. In this study, effects high glucose on potential ability for Rap1b ameliorate these were investigated. kidneys mice, apoptotic dysmorphic mitochondria observed, Bcl-2 expression was decreased, Bax increased....
Objective To construct a life-sized eye model using the three-dimensional (3D) printing technology for fundus viewing study of system. Methods We devised our schematic based on Navarro's and redesigned some parameters because change corneal material implantation intraocular lenses (IOLs). Optical performance was compared with other two reported physical eyes ZEMAX optical design software. With computer aided (CAD) software, we designed 3D digital main structure eye, which used printing....
Objectives Diabetic retinopathy (DR) is a common diabetic eye disease which well-known as the result of microvascular retinal changes. Although potential biological functions astragaloside IV (AS IV) have long been described in traditional system medicine, its protective effect on DR remains unclear. This study aims to investigate function and mechanism AS type 2 db/db mice. Methods Db/db mice were treated with (4.5 mg/kg or 9 mg/kg) physiological saline by oral gavage for 20 weeks along...
Hypothermia therapy has been shown to confer robust protection against brain injury and cardiac arrest. However, the mechanisms underlying endothelial cell of hypothermia have not yet completely elucidated. Here, we investigated molecular effects on tumour necrosis factor-alpha (TNF-alpha)-induced barrier dysfunction apoptosis.Human umbilical vein cells (HUVECs) treated with TNF-alpha were incubated under normothermia (37 degrees C) or (33 C). [corrected] Endothelial permeability, actin...