A5001789535- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Histone Deacetylase Inhibitors Research
- Ubiquitin and proteasome pathways
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- French Urban and Social Studies
- Angiogenesis and VEGF in Cancer
- 14-3-3 protein interactions
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Salivary Gland Disorders and Functions
- Lymphatic System and Diseases
- Protist diversity and phylogeny
- Cell Adhesion Molecules Research
- Neurogenesis and neuroplasticity mechanisms
- Cancer, Hypoxia, and Metabolism
- Protease and Inhibitor Mechanisms
- Ion Transport and Channel Regulation
- Chronic Myeloid Leukemia Treatments
University of Liège
2008-2024
Université Libre de Bruxelles
2020-2024
Utrecht University
2016-2020
Gembloux Agro-Bio Tech
2006-2009
Walter and Eliza Hall Institute of Medical Research
1995
The blood-brain barrier (BBB) protects the central nervous system (CNS) from harmful blood-borne factors. Although BBB dysfunction is a hallmark of several neurological disorders, therapies to restore function are lacking. An attractive strategy repurpose developmental regulators, such as Wnt7a, into BBB-protective agents. However, safe therapeutic use Wnt ligands complicated by their pleiotropic Frizzled signaling activities. Taking advantage Wnt7a/b-specific Gpr124/Reck co-receptor...
Kinesin-1 is responsible for microtubule-based transport of numerous cellular cargoes. Here, we explored the regulation kinesin-1 by MAP7 proteins. We found that all four mammalian family members bind to kinesin-1. In HeLa cells, MAP7, MAP7D1, and MAP7D3 act redundantly enable kinesin-1–dependent microtubule recruitment truncated KIF5B-560, which contains stalk but not cargo-binding autoregulatory regions. vitro, purified increase landing rate processivity through transient association with...
The microtubule cytoskeleton regulates cell polarity by spatially organizing membrane trafficking and signaling processes. In epithelial cells, microtubules form parallel arrays aligned along the apico-basal axis, recent work has demonstrated that members of CAMSAP/Patronin family control apical tethering minus ends. Here, we show in mammalian intestinal spectraplakin ACF7 (also known as MACF1) specifically binds to CAMSAP3 is required for localization CAMSAP3-decorated Loss but not or its...
Microtubules control different aspects of cell polarization. In cells with a radial microtubule system, pivotal role in setting up asymmetry is attributed to the relative positioning centrosome and nucleus. Here, we show that loss had no effect on ability endothelial polarize move 2D 3D environments. contrast, non-centrosomal microtubules stabilized by minus-end-binding protein CAMSAP2 were required for directional migration substrates establishment polarized morphology soft matrices. was...
Intracellular transport relies on multiple kinesins, but it is poorly understood which kinesins are present particular cargos, what their contributions and whether they act simultaneously the same cargo. Here, we show that Rab6-positive secretory vesicles transported from Golgi apparatus to cell periphery by kinesin-1 KIF5B kinesin-3 KIF13B, determine location of secretion events. plays a dominant role, whereas KIF13B helps Rab6 reach freshly polymerized microtubule ends, binds poorly,...
Abstract Vertebrate organs require locally adapted blood vessels 1,2 . The gain of such organotypic vessel specializations is often deemed to be molecularly unrelated the process organ vascularization. Here, opposing this model, we reveal a molecular mechanism for brain-specific angiogenesis that operates under control Wnt7a/b ligands—well-known blood–brain barrier maturation signals 3–5 relies on Wnt7a/b-dependent expression Mmp25, which find enriched in brain endothelial cells. CRISPR–Cas9...
Class IIa histone deacetylases (HDACs) act as key transcriptional regulators in several important developmental programs. Their activities are controlled via phosphorylation-dependent nucleocytoplasmic shuttling. Phosphorylation of conserved serine residues triggers association with 14-3-3 proteins and cytoplasmic relocalization class HDACs, which leads to the derepression their target genes. Although a lot effort has been made toward identification inactivating kinases that phosphorylate...
Abstract Background Human T-cell leukemia virus type 1 (HTLV-1) and 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in dynamic struggle activating antagonistic interactions with host cells. Investigations focused on one or few genes have identified several human factors interacting viral proteins. Most the...
When a T cell and an antigen-presenting form immunological synapse, rapid dynein-driven translocation of the centrosome toward contact site leads to reorganization microtubules associated organelles. Currently, little is known about how regulation microtubule dynamics contributes this process. Here, we show that knockout KIF21B, kinesin-4 linked autoimmune disorders, causes overgrowth perturbs translocation. KIF21B restricts length by inducing pausing typically followed catastrophe....
Within the central nervous system, Wnt7a/b are unambiguously discriminated from other Wnt ligands by an endothelial receptor complex made of glycosylphosphatidylinositol (GPI)-anchored Reck and adhesion G protein-coupled (GPCR) Gpr124. is a Wnt7a/b-specific receptor, while Gpr124 facilitates delivery Reck-bound to Frizzled, through partially characterized mechanisms. We report that, in zebrafish, Gpr124-Frizzled interactions dominated intracellular scaffolds that exploit striking molecular...
Class IIa histone deacetylases (HDACs) are found both in the cytoplasm and nucleus where they repress genes involved several major developmental programs.In response to specific signals, repressive activity of class HDACs is neutralized through their phosphorylation on multiple N-terminal serine residues 14-3-3-mediated nuclear exclusion.Here, we demonstrate that subjected signal-independent export relies constitutive phosphorylation.We identify EMK C-TAK1, two members microtubule...
DUSP3 phosphatase, also known as Vaccinia-H1 Related (VHR) encoded by DUSP3/Dusp3 gene, is a relatively small member of the dual-specificity protein phosphatases. In vitro studies showed that negative regulator ERK and JNK pathways in several cell lines. On other hand, implicated human cancer. It has been alternatively described having tumor suppressive oncogenic properties. Thus, available data suggest plays complex contradictory roles tumorigenesis could be type-dependent. Since most these...
Vascular supply of tumors is one the main targets for cancer therapy. Here, we investigated if plocabulin (PM060184), a novel marine-derived microtubule-binding agent, presents antiangiogenic and vascular-disrupting activities. The effects on microtubule network dynamics were studied HUVEC endothelial cells. We have also its capillary tube structures formation or destabilization in three-dimensional collagen matrices. In vivo experiments performed different tumor cell lines. vitro studies...
Sjögren's syndrome (SS) is an exocrinopathy characterized by the hypofunction of salivary glands (SGs). Aquaporin-5 (AQP5); a water channel involved in saliva formation; aberrantly distributed SS SG acini and contributes to glandular dysfunction. We aimed investigate role ezrin AQP5 mislocalization SGs. The AQP5-ezrin interaction was assessed immunoprecipitation proteome analysis proximity ligation assay immortalized human cells. demonstrated, for first time, between AQP5. A model complex...
Saliva secretion requires effective translocation of aquaporin 5 (AQP5) water channel to the salivary glands (SGs) acinar apical membrane. Patients with Sjögren's syndrome (SS) display abnormal AQP5 localization within cells from SGs that correlate sicca manifestation and hypofunction. Several proteins such as Prolactin-inducible protein (PIP) may regulate trafficking observed in lacrimal mice. However, role AQP5-PIP complex remains poorly understood. In present study, we show PIP interacts...
Cardiovascular disease linked to atherosclerosis is the leading cause of death worldwide. Atherosclerosis mainly dysfunction in vascular endothelial cells and subendothelial accumulation oxidized forms LDL. In present study, we investigated role myeloperoxidase LDL (Mox-LDL) cell dysfunction. We studied effect proinflammatory Mox-LDL treatment on motility, a parameter essential for normal processes such as angiogenesis blood vessel repair. This particularly important context an atheroma...
Rasa3 is a GTPase activating protein of the GAP1 family which targets R-Ras and Rap1. Although catalytic inactivation or deletion in mice leads to severe hemorrhages embryonic lethality, biological function cellular location underlying these defects remains unknown. Here, using combination loss studies mouse zebrafish as well vitro cell biology approaches, we identify key role for endothelial cells vascular lumen integrity. Specific ablation endothelium, but not megakaryocytes platelets,...
Muscle formation is controlled by a number of key myogenic transcriptional regulators that govern stage-specific gene expression programs and act as terminal effectors intracellular signaling pathways. To date, the role phosphatases in cascades instructing muscle development remains poorly understood. Here, we show specific PP2A-B55δ holoenzyme necessary for skeletal myogenesis. The primary to dephosphorylate histone deacetylase 4 (HDAC4) following myocyte differentiation ensure repression...
Lymphangiogenesis, the formation of lymphatic vessels, is tightly linked to development venous vasculature, both at cellular and molecular levels. Here, we identify a novel role for Sorbs1, founding member SoHo family cytoskeleton adaptor proteins, in vascular zebrafish.