A5031515506- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- Cancer-related Molecular Pathways
- Renal and related cancers
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- ATP Synthase and ATPases Research
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Hedgehog Signaling Pathway Studies
- Climate Change Communication and Perception
- Cancer Genomics and Diagnostics
- Genetics, Bioinformatics, and Biomedical Research
- Plant Molecular Biology Research
- 14-3-3 protein interactions
- Genetic Syndromes and Imprinting
- Lipid metabolism and biosynthesis
- Cancer-related gene regulation
- Cancer Cells and Metastasis
- Congenital heart defects research
- Evolution and Genetic Dynamics
Instituto Gulbenkian de Ciência
2016-2025
National Institutes of Health
2017
National Cancer Institute
2017
Center for Cancer Research
2017
Cold Spring Harbor Laboratory
2017
Swedish University of Agricultural Sciences
2012
Cancer Research UK
2004-2007
University of Cambridge
2004-2007
University of Siena
2007
Associação Viver a Ciência
2004
Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated tumor development, is an upstream regulator of biogenesis necessary for centriole formation. We found that overexpression SAK/PLK4 could induce amplification centrioles Drosophila embryos and their de novo formation unfertilized eggs. Both processes required the activity DSAS-6 DSAS-4, two molecules Thus, template-free self-assembly...
The centriole and basal body (CBB) structure nucleates cilia flagella, is an essential component of the centrosome, underlying eukaryotic microtubule-based motility, cell division polarity. In recent years, components CBB-assembly machinery have been identified, but little known about their regulation evolution. Given diversity cellular contexts encountered in eukaryotes, remarkable conservation CBB morphology, we asked whether general mechanistic principles could explain assembly. We...
Abstract Centrosomes are the major microtubule organising centres of animal cells. Deregulation in their number occurs cancer and was shown to trigger tumorigenesis mice. However, incidence, consequence origins this abnormality poorly understood. Here, we screened NCI-60 panel human cell lines systematically analyse centriole structure. Our screen shows that amplification is widespread highly prevalent aggressive breast carcinomas. Moreover, identify another recurrent feature cells: size...
An important feature of fertilization is the asymmetric inheritance centrioles. In most species it sperm that contributes initial centriole, which builds first centrosome essential for early development. However, given centrioles are thought to be exceptionally stable structures, mechanism behind centriole disappearance in female germ line remains elusive and paradoxical. We elucidated a program maintenance fruit flies, led by Polo kinase pericentriolar matrix (PCM): The PCM down-regulated...
Adult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute the functional regeneration of heart. In order understand mechanisms underlying this plasticity we performed longitudinal studies on single cardiomyocytes culture. We find that majority enter S phase, a process occurs response serum-activated pathways is dependent phosphorylation retinoblastoma protein. However, more than half these cells stably arrest at either entry mitosis...
ABSTRACT The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to existing structure (canonical duplication) or de novo. How centrosomes novo not known. master driver biogenesis, PLK4, critical for recruitment several centriole components. Here, we investigate beginning taking advantage Xenopus egg...
Cilia are essential for cell signaling and sensory perception. In many types, a cytoskeletal structure called the ciliary rootlet links cilium to body. Previous studies indicated that rootlets support long-term stability of some cilia. Here we report Drosophila melanogaster Rootletin (Root), sole orthologue mammalian paralogs C-Nap1, assembles into diverse lengths among neuron subtypes. Root mutant neurons lack have dramatically impaired function, resulting in behavior defects associated...