A5061289037- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- Pancreatitis Pathology and Treatment
- Gastric Cancer Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Gastrointestinal Tumor Research and Treatment
- Surgical Simulation and Training
- Neuroendocrine Tumor Research Advances
- Colorectal Cancer Surgical Treatments
- Metastasis and carcinoma case studies
- AI in cancer detection
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Esophageal Cancer Research and Treatment
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Esophageal and GI Pathology
- Microtubule and mitosis dynamics
- Anatomy and Medical Technology
- Cancer, Hypoxia, and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
Kyushu University
2016-2025
Kumamoto University
2014-2025
Kyushu University Hospital
2009-2023
Initiatives (Denmark)
2008-2023
Institut national de recherche en informatique et en automatique
2021
East Asia School of Theology
2021
Chiba University
2017
Seattle University
2017
Pancreas Centre (Canada)
2011-2015
Japan Society for the Promotion of Science
2013-2015
Abstract Radiotherapy represents a major treatment option for patients with pancreatic cancer, but recent evidence suggests that radiation can promote invasion and metastasis of cancer cells. Interactions between cells surrounding stromal may play an important role in aggressive tumor progression. In the present study, we investigated invasive phenotype response to coculture irradiated fibroblasts. Using vitro assay, demonstrated nonirradiated fibroblasts significantly increased ability and,...
Due to the poor prognosis of pancreatic cancer, novel diagnostic modalities for early diagnosis and new therapeutic strategy are urgently needed. Recently, microRNA-21 (miR-21) was reported be strongly overexpressed in cancer as well other solid cancers. We investigated functional roles miR-21, which have not been fully elucidated cancer. miR-21 expression assessed cell lines (14 lines, primary cultures normal epithelial cells fibroblasts, a human ductal line) tissue samples (25 tissues 25...
Importance Evidence of implementation laparoscopic gastrectomy for locally advanced gastric cancer is currently insufficient, as the primary end point in previous prospective studies was evaluated at a median follow-up time 3 years. More robust evidence necessary to verify noninferiority gastrectomy. Objective To compare 5-year survival outcomes between laparoscopy-assisted distal (LADG) and open (ODG) with D2 lymph node dissection cancer. Design, Setting, Participants This multicenter,...
Abstract Background Recently, the microRNA-200 family was reported to affect cancer biology by regulating epithelial mesenchymal transition (EMT). Especially, expression of miR-200c has been shown be associated with upregulating E-cadherin , a gene known involved in pancreatic behavior. However, significance is unknown. Methods In present study, we investigated relationship between and panel 14 cell lines macro-dissected formalin-fixed paraffin-embedded (FFPE) tissue samples obtained from 99...
Pancreatic stellate cells (PSC), which are implicated in desmoplasia pancreatic cancer, enhance the malignancy of cancer and confer resistance to established treatments. We investigated whether antifibrotic agent pirfenidone can suppress exert antitumor effects against cancer. Primary PSCs were from tissue obtained during surgery. In vitro, inhibited proliferation, invasiveness, migration a dose-dependent manner. Although supernatants untreated increased (PCC), pirfenidone-treated decreased...
The standard palliative chemotherapy for pancreatic ductal adenocarcinoma (PDAC) is gemcitabine-based chemotherapy; however, PDAC still presents a major therapeutic challenge. aims of this study were to investigate the expression pattern genes involved in gemcitabine sensitivity resected tissues and determine correlations gene with treatment outcome.We obtained formalin-fixed paraffin-embedded (FFPE) tissue samples from 70 patients PDAC. Of patients, 40 received adjuvant (AC). We measured...
Abstract Background Interactions between cancer cells and surrounding cancer-associated fibroblasts (CAFs) play an important role in progression. Invasive ductal carcinoma (IDC) of the pancreas is characterized by abundant fibrous connective tissue called desmoplasia. Podoplanin (PDPN) a lymphatic vessel marker (D2-40), expression PDPN stromal CAFs has been reported to be prognostic indicator various types cancer. Methods Expression pancreatic IDCs was assessed immunohistochemical...
To identify a high-risk group of patients with pancreatic ductal adenocarcinoma (PDAC), independently arising in the pancreas intraductal papillary mucinous neoplasm (IPMN), using histopathologic subtypes.Pathologic features IPMN distinct PDAC, including subtypes and PDAC phenotypes, have not been well characterized. Mucin expression patterns mutational status GNAS KRAS are useful to explore relationship between these 2 lesion types.Clinicopathologic data 179 resected IPMNs 180 PDACs without...
Background Necroptosis is a form of programmed cell death that accompanied by release intracellular contents, and reportedly contributes to various diseases. Here, we investigate the significance necroptosis in pancreatic cancer. Methods We used immunohistochemistry western blot analysis evaluate expression key mediators necroptosis—receptor-interacting serine/threonine protein kinase 3 (RIP3) mixed lineage domain-like (MLKL)—in human also tested effects conditioned media (CM) from...
Extracellular signal-regulated kinases (ERKs) have been related to multiple cancers, including breast cancer, hepatocellular lung cancer and colorectal cancer. ERK1/2 inhibitor can suppress growth of KRAS-mutant pancreatic tumors by targeting cell. However, no studies shown the expression on stromal its effect cancer-stromal interaction.Immunohistochemistry western blotting were performed detect p-ERK1/2 in tissues cells. Cell viability assay was used study IC50 ERK cells (PCCs) primary...
Cancer‑associated fibroblasts (CAFs) promote the progression of pancreatic ductal adenocarcinoma (PDAC) via tumor‑stromal interactions. Neutrophil extracellular traps (NETs) are DNA meshworks released from neutrophils together with proteolytic enzymes against foreign pathogens. Emerging studies suggest their contribution to liver metastasis in several types cancer. Herein, order investigate role NETs PDAC, effects NET inhibitors on spontaneous PDAC mouse models were evaluated. It was...
Intratumor bacteria modify the tumor immune microenvironment and influence outcomes of various tumors. Periodontal pathogen Fusobacterium nucleatum has been detected in pancreatic cancer tissues is associated with poor prognosis. However, it remains unclear how F. affects cancer. Here, we compared clinical features colonization tissues. was 15.5% (13/84) patients. The size significantly larger nucleatum-positive group than negative group. To clarify biological effect intratumor on...
Abstract Recent microarray analyses showed that the S100 family contains members are candidate diagnostic markers or therapeutic targets. In present study, to evaluate involvement of S100A6 in pancreatic cancer and its clinical usefulness for diagnosis, we examined mRNA expression tissues juice from patients with different diseases. To investigate role carcinogenesis potential as a marker early detection cancer, did immunohistochemistry microdissection-based analysis normal ducts,...
The microRNA-17-92 cluster is an oncogene in human B cell lymphomas and lung cancers. Previous microRNA microarray data revealed that miR-17-5p, a member of the miR-17-92 cluster, upregulated pancreatic cancer. However, involvement miR-17-5p expression carcinogenesis has not well been studied. In present study, we measured levels cancer lines, primary cultures normal ductal cells, formalin-fixed paraffin-embedded (FFPE) tissue samples derived from 80 patients who underwent pancreatectomy for...
OBJECTIVES: Pancreatic ductal adenocarcinoma (PDAC) is often characterized by a prominent desmoplastic stroma that induced partially alpha-smooth muscle actin (SMA)-expressing activated pancreatic stellate cells (PSCs). This study aimed to investigate the significance of alpha-SMA expression in PDAC and correlation between mRNA levels patient prognosis. METHODS: We obtained formalin-fixed, paraffin-embedded tissue samples from 109 patients with PDAC, who underwent pancreatectomy at our...
To improve the prognosis of patients after resection pancreatic cancer, most appropriate and efficient treatment should be provided to specific subsets patients. Our aim was identify promising microRNAs as markers predict responses gemcitabine in with resected cancer.Two gemcitabine-resistant cancer cell lines were established, global microRNA expression analyses performed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Eleven miRNAs selected putative predictive...