A5012986870- Pancreatic and Hepatic Oncology Research
- Autophagy in Disease and Therapy
- Cancer Genomics and Diagnostics
- Pancreatitis Pathology and Treatment
- Phagocytosis and Immune Regulation
- Epigenetics and DNA Methylation
- Neuroendocrine Tumor Research Advances
- Cancer Immunotherapy and Biomarkers
- Adenosine and Purinergic Signaling
- Radiomics and Machine Learning in Medical Imaging
- Cancer Cells and Metastasis
- Gallbladder and Bile Duct Disorders
- Renal cell carcinoma treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Molecular Biology Techniques and Applications
- Cancer Research and Treatments
- Gastric Cancer Management and Outcomes
- Intraperitoneal and Appendiceal Malignancies
- Pediatric Hepatobiliary Diseases and Treatments
- Gastrointestinal disorders and treatments
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Pancreatic function and diabetes
- Inflammatory Biomarkers in Disease Prognosis
- Glycosylation and Glycoproteins Research
Kyushu University
2000-2025
Kitakyushu Municipal Medical Center
2020-2024
Johns Hopkins Medicine
2018-2024
Johns Hopkins University
2018-2024
Cancer Research Center
2019-2023
Muroran Institute of Technology
2023
Deutschen Konsortium für Translationale Krebsforschung
2020
Yamaguchi Prefecture Central Hospital
2019
Seattle University
2017
Kawasaki Medical School
2013-2014
Vascular endothelial growth factor-C (VEGF-C) functions specifically to induce lymphangiogenesis. We examined the relationship between expression of VEGF-C and clinicopathological features in patients with colorectal cancer. The 99 primary tumours 18 metastatic lymph nodes from cancer was immunohistochemically. To verify mRNA expression, reverse transcriptase-polymerase chain reaction (RT-PCR) carried out. correlated lymphatic involvement, metastasis, depth invasion. On other hand,...
Background Necroptosis is a form of programmed cell death that accompanied by release intracellular contents, and reportedly contributes to various diseases. Here, we investigate the significance necroptosis in pancreatic cancer. Methods We used immunohistochemistry western blot analysis evaluate expression key mediators necroptosis—receptor-interacting serine/threonine protein kinase 3 (RIP3) mixed lineage domain-like (MLKL)—in human also tested effects conditioned media (CM) from...
Extracellular signal-regulated kinases (ERKs) have been related to multiple cancers, including breast cancer, hepatocellular lung cancer and colorectal cancer. ERK1/2 inhibitor can suppress growth of KRAS-mutant pancreatic tumors by targeting cell. However, no studies shown the expression on stromal its effect cancer-stromal interaction.Immunohistochemistry western blotting were performed detect p-ERK1/2 in tissues cells. Cell viability assay was used study IC50 ERK cells (PCCs) primary...
To compare the risk of neoplastic progression by germline mutation status versus family history without a known (familial risk) among individuals with an increased for pancreatic cancer who are undergoing surveillance.Of 464 high-risk in Cancer Pancreas Screening program at Johns Hopkins Hospital were surveillance, 119 had deleterious susceptibility gene; 345 met criteria surveillance but not to harbor mutation. We used next-generation sequencing identify previously unrecognized mutations...
The tumor microbiome of patients with pancreas ductal adenocarcinoma (PDAC) includes bacteria normally present in the upper gastrointestinal tract. If predominant source intratumoral PDAC is retrograde migration from duodenum, duodenal fluid could be a representative biospecimen for determining profiles or at risk developing PDAC.We performed case-control study comparing bacterial and fungal (16S 18S rRNA) secretin-stimulated collections 308 undergoing endoscopy including 134 normal control...
Intratumor bacteria modify the tumor immune microenvironment and influence outcomes of various tumors. Periodontal pathogen Fusobacterium nucleatum has been detected in pancreatic cancer tissues is associated with poor prognosis. However, it remains unclear how F. affects cancer. Here, we compared clinical features colonization tissues. was 15.5% (13/84) patients. The size significantly larger nucleatum-positive group than negative group. To clarify biological effect intratumor on...
To evaluate whether germline variants in genes encoding pancreatic secretory enzymes contribute to cancer susceptibility, we sequenced the coding regions of CPB1 and other known pancreatitis susceptibility (PRSS1, CPA1, CTRC, SPINK1) a hospital series cases controls. Variants CPB1, CPA1 (encoding carboxypeptidase B1 A1), CTRC were evaluated second set with familial More deleterious variants, defined as having impaired protein secretion induction endoplasmic reticulum (ER) stress transfected...
AimsAlthough dietary saturated fatty acids (SFA) are considered atherogenic, associations between SFAs intake and stroke coronary heart disease still debated. We sought to test the hypothesis that SFA is associated inversely with risk of its subtypes positively among Japanese, whose average lower than Westerners.
Abstract Purpose: CA19–9 synthesis is influenced by common variants in the fucosyltransferase (FUT) enzymes FUT3 and FUT2. We developed a clinical test to detect FUT variants, evaluated its diagnostic performance for pancreatic ductal adenocarcinoma (PDAC). Experimental Design: A representative set of controls from Cancer Pancreas Screening study was identified each functional group. Diagnostic sensitivity determined first testing 234 PDAC cases, followed 134-case validation set, all whom...
Although immune checkpoint blockade (ICB) therapy has proven to be extremely effective at managing certain cancers, its efficacy in treating pancreatic ductal adenocarcinoma (PDAC) been limited. Therefore, enhancing the effect of ICB could improve prognosis PDAC. In this study, we focused on histamine receptor H1 (HRH1) and investigated impact for We assessed HRH1 expression cancer cell (PCC) specimens from PDAC patients through public data analysis immunohistochemical (IHC) staining. The...
Pancreatic cancer progression involves components of the tumor microenvironment, including stellate cells, immune endothelial and extracellular matrix. Although peripancreatic fat is main stromal component involved in extra-pancreatic invasion, its roles local invasion metastasis pancreatic remain unclear. This study investigated role adipose tissue using genetically engineered mice (Pdx1-Cre; LSL-KrasG12D; Trp53R172H/+) an vitro model organotypic invasion. Mice fed a high diet had...
Levels of carcinoembryonic antigen (CEA), carbohydrate 19-9 (CA19-9), and cancer 125 (CA-125) in blood are used as markers to determine the response patients with therapy, but not identify pancreatic cancer.We obtained samples from 504 undergoing surveillance 2002 through 2018 who did develop measured levels tumor CA19-9, CEA, CA-125, thrombospondin-2. Single-nucleotide polymorphisms (SNPs) FUT3, FUT2, ABO, GAL3ST2 that have been associated were establish SNP-defined ranges for each marker....
Pancreatic ductal adenocarcinoma (PDAC) is characterized by abundant stroma in which microenvironmental (niche) factors promote PDAC progression. In mouse models, reduction of the increased proportion poorly differentiated with a worse prognosis. Here, we aimed to clarify effects on that may define phenotype and induce distinct therapeutic responses.The molecular features based differentiation grade were clarified genome transcriptome analysis using organoids (PDOs). We identified dependency...
Although recent studies revealed that adipose tissue accelerates pancreatic tumor progression with excessive extracellular matrix, key players for desmoplasia in the microenvironment remains unknown. Here, we investigated roles of tissue-derived stromal cells (ASCs) desmoplastic lesions and by vitro vivo experiments. In a three-dimensional (3-D) organotypic fat invasion model using visceral from CAG-EGFP mice, GFP-positive fibroblastic infiltrated toward cancer cells. When were inoculated...
Pancreatic ductal adenocarcinoma (PDAC) is considered a "cold" tumor because the immune microenvironment (TIME) exhibits poor intratumoral T-cell infiltration. This study aimed to identify marker genes associated with induction of cold TIME in PDAC cells. We orthotopically transplanted 10 primary cultures derived from KrasG12D/+; Trp53R172H/+; Pdx-1-Cre (KPC) mice into immunocompetent and evaluated by immunohistochemistry (IHC) staining CD8. divided two groups: group low CD8+ infiltration...
Pancreatic cancer shows very poor prognosis and high resistance to conventional standard chemotherapy immunotherapy; therefore, the development of new breakthrough therapies is highly desirable. We retrospectively evaluated safety efficacy neoantigen peptide-pulsed dendritic cell (Neo-P DC) vaccine therapy after surgical treatment pancreatic cancer. The result showed induction neoantigen-specific T cells in 13 (81.3%) 16 patients who received Neo-P DC vaccines. In survival analysis nine...