A5101492640- Metabolomics and Mass Spectrometry Studies
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Colorectal Cancer Treatments and Studies
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Tea Polyphenols and Effects
- Cancer, Hypoxia, and Metabolism
- Cancer Treatment and Pharmacology
- RNA modifications and cancer
- Metastasis and carcinoma case studies
- Isotope Analysis in Ecology
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Mitochondrial Function and Pathology
- Enzyme-mediated dye degradation
- Advanced Proteomics Techniques and Applications
- Synthesis and properties of polymers
- Pancreatic and Hepatic Oncology Research
- Adipose Tissue and Metabolism
- Glioma Diagnosis and Treatment
- Synthesis and biological activity
- Biochemical and Molecular Research
- Insect Utilization and Effects
- Epigenetics and DNA Methylation
National Institute of Advanced Industrial Science and Technology
2010-2025
Biomedical Research Institute
2025
Kyushu University
2012-2023
Kyushu University Hospital
2016-2017
Maruishi Pharmaceutical (Japan)
2016
Fukuoka University
2015
Institute of Navigation
2014
Yamaguchi University
2003-2011
Kyoto Institute of Technology
1994-1996
Abstract Sarcopenia is associated with increased morbidity and mortality in chronic kidney disease (CKD). Pathogenic mechanism of skeletal muscle loss CKD, which defined as uremic sarcopenia, remains unclear. We found that causative pathological sarcopenia metabolic alterations by toxin indoxyl sulfate. Imaging mass spectrometry revealed sulfate accumulated tissue a mouse model CKD. Comprehensive metabolomics induces such upregulation glycolysis, including pentose phosphate pathway...
A sensitive and simultaneous analytical technique for visualizing multiple endogenous molecules is now strongly required in biological science. Here, we show the applicability of a matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) system getting chemically diverse metabolite profiles on single-mammalian cell. This ultrahighly MALDI-MS enabled spatially resolved detection broad range metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids,...
Microglia have been implicated in various neurological and psychiatric disorders rodent human postmortem studies. However, the dynamic actions of microglia living brain not clarified due to a lack studies dealing with situ microglia. Herein, we present novel technique for developing induced microglia-like (iMG) cells from peripheral blood cells. An optimized cocktail cytokines, GM-CSF IL-34, converted monocytes into iMG within 14 days. The microglial characterizations; expressing markers,...
Evaluating the severity of depression (SOD), especially suicidal ideation (SI), is crucial in treatment not only patients with mood disorders but also psychiatric general. SOD has been assessed on interviews such as Hamilton Rating Scale for Depression (HAMD)-17, and/or self-administered questionnaires Patient Health Questionnaire (PHQ)-9. However, these evaluation systems have relied a person’s subjective information, which sometimes lead to difficulties clinical settings. To resolve this...
Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research its industrialization. This study set out establish standards-based solutions improve the accuracy reproducibility metagenomics-based profiling fecal samples.
Green tea has various health promotion effects. Although there are numerous cultivars, little is known about the differences in their nutraceutical properties. Metabolic profiling techniques can provide information on relationship between metabolome and factors such as phenotype or quality. Here, we performed metabolomic analyses to explore health-promoting attributes (bioactivity) of diverse Japanese green cultivars.We investigated ability leaf extracts from 43 cultivars inhibit...
Elevated circulating uremic toxins are associated with a variety of symptoms and organ dysfunction observed in patients chronic kidney disease (CKD). Indoxyl sulfate (IS) p-cresyl (PCS) representative that exert various harmful effects. We recently showed IS induces metabolic alteration skeletal muscle causes sarcopenia mice. However, whether organ-specific accumulation PCS is tissue still unclear. investigated the using liquid chromatography/tandem mass spectrometry tissues from mice...
We propose a novel strategy for determining the elemental composition of organic compounds using peak ratio isotopic fine structure observed by high-magnetic field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). Using 3′-phosphoadenosine 5′-phosphosulfate and CTU guanamine as standard compounds, peaks derived from 15N-, 34S-, 18O-substituted forms were separated 13C-substituted species. Furthermore, these quantitatively detected closely matched natural abundance each...
In the present study, a high-throughput and nontargeted metabolomic technique using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) was developed for rapid analysis of cellular metabolites. Either detection limit or linearity between concentrations several standards peak intensities examined, indicating lower than 10 fmol/well with high at low concentrations. To verify validity this method, metabolites from human acute lymphoblastic leukemia Jurkat cells were...
Pancreatic cancer progression involves components of the tumor microenvironment, including stellate cells, immune endothelial and extracellular matrix. Although peripancreatic fat is main stromal component involved in extra-pancreatic invasion, its roles local invasion metastasis pancreatic remain unclear. This study investigated role adipose tissue using genetically engineered mice (Pdx1-Cre; LSL-KrasG12D; Trp53R172H/+) an vitro model organotypic invasion. Mice fed a high diet had...
Spatiotemporal information about biomolecules is indispensable for precise pathological analysis, but it remains largely unclear. Here we show a novel analytical platform combing mass spectrometry imaging (MSI) with its complementary technique, liquid chromatography-mass (LC-MS), to elucidate more comprehensive metabolic behaviors, spatiotemporal information, in tissues. Analysis of rat transient middle cerebral artery occlusion (MCAO) brain tissue after ischemia-reperfusion was performed...
Understanding the spatial distribution of bioactive small molecules is indispensable for elucidating their biological or pharmaceutical roles. Mass spectrometry imaging (MSI) enables determination ionizable present in tissue sections whole-body single heterogeneous organ samples by direct ionization and detection. This emerging technique now widely used situ label-free molecular endogenous exogenous molecules. MSI allows simultaneous visualization many types including a parent molecule its...
The only property of reinforcement insects are commonly thought to learn about is its value. We show that larval Drosophila not remember the value (How much?), but also quality (What?). This demonstrated both within appetitive domain by using sugar vs amino acid as different reward qualities, and aversive bitter high-concentration salt qualities punishment. From available literature, such nuanced memories for unexpected pose a challenge present models how insect memory organized. Given...
Oxidative dysfunction in the metabolism has long been implicated diverse biological disorders. Although a substantial number of metabolic enzymes are targeted for inactivation by oxidative stress, identifying those targets remains difficult due to lack comprehensive observations acting through stress response. We herein developed metabolomics strategy using integrative liquid chromatography‐mass spectrometry ( LC ‐ MS ) and observing rapid metabolomic changes response hydrogen peroxide (H 2...
ABSTRACT Mapping brain function to structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva simple enough be tractable, yet complex interesting. It features about 10,000 neurons and capable of various taxes, kineses Pavlovian conditioning. All its are currently being mapped into light-microscopical atlas, Gal4 strains generated experimentally access one at time. In addition, electron microscopic reconstruction nervous system seems within reach. Notably,...
Information on spatiotemporal metabolic behavior is indispensable for a precise understanding of physiological changes and responses, including those ripening processes wounding stress, in fruit, but such information still limited. Here, we visualized the spatial distribution metabolites within tissue sections tomato (Solanum lycopersicum L.) fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) technique combined with matrix...
In the present study, a high-throughput analytical method for intracellular metabolites using MALDI−MS has been developed. As an tool, quantitative performance and dynamic range of MALDI−TOF−MS was confirmed to be suitable characterizing trends metabolism. The technique tested by investigating metabolism Escherichia coli analyzing whole cell samples taken consecutively before after perturbation environmental carbon source. result, dramatic changes metabolite concentrations responding were...
Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) is a powerful technique to visualize the distributions of biomolecules without any labeling. In MALDI-MSI experiments, choice matrix deposition method important for acquiring favorable MSI data with high sensitivity and reproducibility. Generally, manual or automated spray-coating sublimation methods are used, but these have some drawbacks respect detection sensitivity, spatial resolution, Herein, we present...
Abstract Although understanding the high-resolution spatial distribution of bioactive small molecules is indispensable for elucidating their biological or pharmacological effects, there has been no analytical technique that can easily detect naïve molecular localization in mammalian tissues. We herein present a novel situ label-free imaging visualizing molecules, using polyphenol. established 1,5-diaminonaphthalene (1,5-DAN)-based matrix-assisted laser desorption/ionization-mass spectrometry...
In mass spectrometry (MS)-based metabolomics studies, reference-free identification of metabolites is still a challenging issue. Previously, we demonstrated that the elemental composition (EC) could be unambiguously determined using isotopic fine structure, observed by ultrahigh resolution MS, which provided relative abundance (RIA) 13C, 15N, 18O, and 34S. Herein, evaluated efficacy RIA for determining ECs based on MS peaks 20,258 known metabolites. The were simulated with ≤25% error in peak...
Activation of acid sphingomyelinase (ASM) leads to ceramide accumulation and induces apoptotic cell death in cancer cells. In the present study, we demonstrate that activation ASM by targeting cancer-overexpressed 67-kDa laminin receptors (67LR) lipid raft disruption inhibits receptor tyrosine kinase (RTK) multiple myeloma Sphingosine 1 (SphK1), a negative regulator with antiapoptotic effects, was markedly elevated The silencing SphK1 potentiated effects green tea polyphenol...