A5035993107- Cancer-related gene regulation
- RNA modifications and cancer
- Cancer, Lipids, and Metabolism
- Epigenetics and DNA Methylation
- melanin and skin pigmentation
- Skin Protection and Aging
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Biochemical Analysis and Sensing Techniques
- Cancer, Hypoxia, and Metabolism
- Medical Imaging and Pathology Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Diet and metabolism studies
- Methane Hydrates and Related Phenomena
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Occupational exposure and asthma
- Pharmacogenetics and Drug Metabolism
- Molecular Biology Techniques and Applications
- Microbial Community Ecology and Physiology
- Occupational and environmental lung diseases
- Paleontology and Stratigraphy of Fossils
- Freezing and Crystallization Processes
- Nuclear Physics and Applications
- Plant Stress Responses and Tolerance
Shimadzu (Japan)
2008-2024
Taiho Pharmaceutical (Japan)
2019
Chiba University
2019
Tottori University
2019
Hoshi University
2019
National Institutes of Health
2019
Japan Organization of Occupational Health and Safety
2019
Koshien University
2012
Kyoto Institute of Technology
2002
Abstract Triple-negative breast cancer (TNBC) chemoresistance hampers the ability to effectively treat patients. Identification of mechanisms driving can lead strategies improve treatment. Here, we revealed that protein arginine methyltransferase-1 (PRMT1) simultaneously methylates D-3-phosphoglycerate dehydrogenase (PHGDH), a critical enzyme in serine synthesis, and glycolytic enzymes PFKFB3 PKM2 TNBC cells. 13C metabolic flux analyses showed PRMT1-dependent methylation these three diverts...
Matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) is a powerful technique to visualize the distributions of biomolecules without any labeling. In MALDI-MSI experiments, choice matrix deposition method important for acquiring favorable MSI data with high sensitivity and reproducibility. Generally, manual or automated spray-coating sublimation methods are used, but these have some drawbacks respect detection sensitivity, spatial resolution, Herein, we present...
Resistant maltodextrin (RMD) is a soluble dietary fiber ingredient whose physiological functions are well recognized in Foods for Specified Health Use (FOSHU) maintaining healthy intestinal regularity, blood glucose levels, and serum lipids. However, its efficacy on combined health risks—metabolic syndrome—was not studied yet. In this study the of RMD humans with metabolic syndrome was investigated. A randomized double-blind placebo-controlled parallel-group trial conducted. Thirty subjects...
Plant hormones act as important signaling molecules that regulate responses to abiotic stress well plant growth and development. Because their concentrations of control the physiological in target tissue, it is know distributions tissues. However, difficult determine hormone concentration on tissue a result limitations conventional methods. Here, we report first multi-imaging two hormones, one cytokinin [i.e., trans-zeatin (tZ)] abscisic acid (ABA) using new technology, matrix-assisted laser...
IntroductionLeptomeningeal carcinomatosis (LMC) occurs frequently in anaplastic lymphoma kinase (ALK)–rearranged NSCLC and develops acquired resistance to ALK tyrosine inhibitors (ALK TKIs). This study aimed clarify the mechanism alectinib, a second-generation TKI, LMC test novel therapeutic strategy.MethodsWe induced alectinib an mouse model with ALK-rearranged cell line, A925LPE3, by continuous oral treatment, established A925L/AR cells. Resistance mechanisms were analyzed using several...
Two recent studies have demonstrated that clotrimazole, a potent antifungal agent, inhibits the growth of chloroquine-resistant strains malaria parasite, Plasmodium falciparum, in vitro. We explored mechanism antimalarial activity clotrimazole relation to hemoglobin catabolism parasite. Because free heme produced from is highly toxic parasite protects itself by polymerizing into insoluble nontoxic hemozoin or decomposing coupled reduced glutathione. shown has high binding affinity for...
This study aims to achieve high spatial-resolution tandem mass spectrometry (MS/MS) imaging for depicting longitudinal and transverse distribution of drugs in hair, which can provide indispensable information the proper interpretation hair test results, including mechanism drug incorporation into hair. Two types samples were obtained analyzed: User's Hair, sampled from a volunteer who took an over-the-counter medicine containing methoxyphenamine (MOP), nonregulated analogue methamphetamine;...
This study aimed to identify single nucleotide polymorphism (SNP) alleles at multiple loci associated with racial differences in skin color using SNP genotyping.A total of 122 Caucasians Toledo, Ohio and 100 Mongoloids Japan were genotyped for 20 SNPs 7 candidate genes, encoding the Agouti signaling protein (ASIP), tyrosinase-related 1 (TYRP1), tyrosinase (TYR), melanocortin receptor (MC1R), oculocutaneous albinism II (OCA2), microphthalmia-associated transcription factor (MITF), myosin VA...
The application of matrix-assisted laser desorption/ionization (MALDI)-based mass spectrometry (MS) to the proteomic analysis formalin-fixed paraffin-embedded (FFPE) tissue presents significant technical challenges. In situ enzymatic digestion is frequently used unlock tissues for analysis, but results are often unsatisfactory. Here, we report a new, simplified in pretreatment method preparing sections MS that involves heating with vapor containing acetonitrile small airtight pressurized...
Induction of cytochrome P450 enzyme 3A (CYP3A) in response to pregnane X receptor (PXR) activators shows species-specific differences. To study the induction human CYP3A PXR activators, we generated a double-humanized mouse model and CYP3A. CYP3A-humanized mice by using artificial chromosome (MAC) vector containing entire genomic locus (hCYP3A-MAC line) were bred with PXR-humanized which ligand-binding domain was replaced that PXR, resulting (hCYP3A-MAC/hPXR line). Oral administration...
Chemosynthetic organisms flourish around deep-sea hydrothermal vents where energy-rich fluids are emitted from metal sulfide chimneys. However, microbial life hosted in mineral assemblages extinct chimneys lacking fluid venting remains largely unknown. The interior of anoxic the percolation oxygenated seawater is limited within tightly packed grains. Given scarcity photosynthetic organics deep seawater, anaerobic microbes might inhabit grain boundaries energetically depending on substrates...
Abstract Imaging mass spectrometry (IMS) has been rarely used to examine specimens of human brain tumours. In the current study, high quality tumour samples were selected by tissue observation. Further, IMS analysis was combined with a new hierarchical cluster (IMS-HCA) and region interest (IMS-ROI). IMS-HCA successful in creating groups consisting similar signal distribution images glial fibrillary acidic protein (GFAP) related multiple proteins primary This clustering data suggested...
In clinical practice, pregabalin is orally administered for neuropathic pain, but causes severe central nervous system side effects, such as dizziness, which results in dose limitation or discontinuation. To reduce the effects of pregabalin, we developed four preparations transdermal application: 0.4% aqueous solution, pluronic lecithin organogel (PLO gel), hydrophilic cream, and lipophilic cream. Transdermal permeabilities among formulations were compared vitro using hairless mouse skin....
This study was undertaken to clarify the molecular basis for human skin color variation and environmental adaptability ultraviolet irradiation, with ultimate goal of predicting impact changes in future environments on health risk. One hundred twenty-two Caucasians living Toledo, Ohio participated. Back cheek were assayed melanin as a quantitative trait marker. Buccal cell samples collected used DNA extraction. SNP genotyping using Masscode system, which entails two-step PCR amplification...
Abstract Diagnostic markers of malignant mesothelioma (MM) have been extensively investigated. Immunohistochemistry (IHC) markers, such as calretinin, used for pathologic diagnosis. However, more diagnostic are required to improve the specificity and sensitivity This study proposed two proteins epithelioid MM. One is RhoA, an MM mutation-susceptible locus-derived protein, another vigilin, a lung small cell carcinoma marker. IHC was performed using 93 (epithelioid, 71 cases; sarcomatoid, 13...
<p>Methods and References</p>
<p>Fig.S4</p>
<p>Fig.S8</p>
<p>Fig.S12</p>
<p>Fig.S9</p>
<p>Fig.S13</p>