A5031034809- Systemic Lupus Erythematosus Research
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Viral Infections and Immunology Research
- Lipoproteins and Cardiovascular Health
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Radiopharmaceutical Chemistry and Applications
- Glycosylation and Glycoproteins Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Galectins and Cancer Biology
- Antioxidant Activity and Oxidative Stress
- Blood disorders and treatments
- Immunodeficiency and Autoimmune Disorders
- Inflammasome and immune disorders
- Cardiomyopathy and Myosin Studies
- Reproductive System and Pregnancy
- Cholesterol and Lipid Metabolism
- Lipid metabolism and disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chronic Lymphocytic Leukemia Research
- Heparin-Induced Thrombocytopenia and Thrombosis
- Boron Compounds in Chemistry
Okayama University
2016-2025
University of Indonesia
2025
Kameda Medical Center
2025
Kagoshima University Hospital
2018
Collaborative Research Group
2014
Corgenix (United States)
2014
Hokkaido University
1993-2010
Health Sciences University of Hokkaido
2010
Dalian University
2010
Azienda Ospedaliera S.Giuseppe Moscati
2008
Anticardiolipin antibodies (aCL) derived from the sera of individuals exhibiting antiphospholipid syndrome (APS) directly bind to beta 2-glycoprotein I (beta 2-GPI), which is adsorbed an oxidized polystyrene surface. Oxygen atoms were introduced on a surface by irradiation with electron or gamma-ray radiation. X-ray photoelectron spectroscopy revealed irradiated surfaces generate C-O and C = O moieties. aCL either APS patients (NZW x BXSB)F1 mice bound 2-GPI coated plates, depending...
Anticardiolipin antibodies (aCL) found in sera from patients with SLE react cardiolipin (CL) the presence of a 50-kDa serum cofactor. The cofactor, which was identified to be beta 2-glycoprotein I by sequencing N-terminal amino acids, not only enhances CL binding but also depresses it associated syphilis. Cofactor-dependent aCL solid phase competitively inhibited simultaneous addition fluid unaffected either prior or high excess Binding syphilis cofactor CL. reacted CL, PS, and PA In...
Objective To clarify the association of autoantibodies against prothrombin with clinical manifestations antiphospholipid syndrome (APS) and presence lupus anticoagulant (LAC). Methods We examined 265 patients who visited our autoimmune disease clinic. IgG IgM antiprothrombin antibodies were tested by enzyme-linked immunosorbent assay (ELISA) as either antiphosphatidylserine–prothrombin complex (aPS/PT) or coated on irradiated ELISA plates (as antigen) (aPT). IgG, IgM, IgA anticardiolipin...
Pulmonary surfactant protein D (SP-D) is a hydrophilic glycoprotein with reduced molecular mass of 43 kDa and member the C-type lectin superfamily, along mannose-binding proteins A (SP-A). We have recently prepared monoclonal antibodies against human SP-D developed an enzyme-linked immunosorbent assay (ELISA). In this study, levels in sera bronchoalveolar lavage (BAL) fluids patients lung diseases were determined by ELISA, using recombinant as standard. demonstrated that concentrations are...
Anticardiolipin antibodies (aCL) in the sera of patients with antiphospholipid syndrome (APS) recognize an altered structure beta 2-glycoprotein I (beta 2-GPI) interacting solid-phase negatively charged phospholipids. 2-GPI bound to Cu2+-oxidized plasma lipoproteins, i.e. oxidized very low-density lipoprotein (oxVLDL), (oxLDL), or high-density (oxHDL). inhibited vitro uptake, cell surface binding, cellular association, and proteolytic degradation oxLDL by murine macrophage J774A.1 cells. The...
Abstract Objective . To investigate whether anticardiolipin antibodies (aCL) in patients with systemic lupus erythematosus (SLE) bind to β 2 ‐glycoprotein I (β GPI), and search for a relationship between the presence of IgG and/or IgM anti‐β GPI antibody clinical manifestations SLE patients. Methods 308 Japanese were measured using phospholipid‐independent enzyme immunoassays. Relationships histories various laboratory data examined. Results The values aCL, as by conventional immunoassay,...
Abstract Emerging evidence has suggested environmental factors as causative agents in the pathogenesis of primary biliary cirrhosis (PBC). We have hypothesized that PBC lipoyl domain immunodominant E2 component pyruvate dehydrogenase (PDC-E2) is replaced by a chemical xenobiotic mimic, which sufficient to break self-tolerance. To address this hypothesis, based upon our quantitative structure-activity relationship data, total 107 potential mimics were coupled lysine residue 15 amino acid...
Beta2-glycoprotein I (beta2-GPI) is a major antigen for antiphospholipid antibodies (Abs, aPL) present in patients with syndrome (APS). We recently reported (J. Lipid Res., 42: 697, 2001; J. 43: 1486, 2002) that beta2-GPI specifically binds to Cu2+-oxidized LDL (oxLDL) and the ligands are omega-carboxylated 7-ketocholesteryl esters. In study, we demonstrate oxLDL forms stable nondissociable complexes serum, high serum levels of associated arterial thrombosis APS. A conjugated ketone function...
NZW x BXSB F1 (W/B F1) male mice develop systemic lupus-like disease, and several autoantibodies, circulating immune complexes, lupus nephritis become apparent. The abnormally high incidence of degenerative coronary vascular disease with myocardial infarction thrombocytopenia due to the presence both platelet-associated antibodies antiplatelet in this animal has been reported. We found that W/B produced autoantibodies against cardiolipin (aCL) titer aCL increases age. from were mainly IgG...
Boron (B) neutron capture therapy (BNCT) is a novel non-invasive targeted cancer based on the nuclear reaction 10B (n, alpha) 7Li that enables death of cells without damaging neighboring normal cells. However, development clinically approved boron drugs remains challenging. We have previously reported self-forming nanoparticles for drug delivery consisting biodegradable polymer, namely, “AB-type” Lactosome® (AB-Lac particles)- highly loaded with hydrophobic B compounds, namely o-Carborane...
Abstract Objective . To elucidate the specificity of anticardiolipin antibodies (aCL) from patients with antiphospholipid syndrome (APS) to various phospholipids (PLs), DNA, and β 2 ‐glycoprotein I (β ‐GPI). Methods Five monoclonal aCL were established peripheral blood lymphocytes 3 APS. The reactivity PLs, ‐GPI was examined by enzyme‐linked immunosorbent assay (ELISA). Results All bound anionic only in presence ‐GPI. Neither nor DNA. Monoclonal solid‐phase on polystyrene ELISA plates that...
β2-Glycoprotein I (β2-GPI) is a major antigen for antiphospholipid antibodies (Abs) present in patients with the syndrome (APS). We previously reported that β2-GPI specifically binds to oxidized low density lipoprotein (oxLDL), but not native (LDL). In study, ligand specific β2-GPI, oxLig-1, was purified from extracted lipids of oxLDL. The structure oxLig-1 shown be identical synthesized 7-ketocholesteryl-9-carboxynonanoate by mass spectroscopy and nuclear magnetic resonance analyses. Both...
beta2-Glycoprotein I (beta2GPI), a plasma glycoprotein with phospholipid-binding property, is known to be the actual target antigen for autoimmune type anticardiolipin antibodies (aCLs). Certain groups of aCLs (anti-beta2GPI antibodies) exert lupus anticoagulant (LA) activity and perturb function vascular endothelial cells. This investigation aimed at highlighting some insights into molecular basis by which their biological effects using anti-beta2GPI mAbs well-characterized epitopes from...
Our aim was to elucidate prospectively whether β2-glyco-protein I-dependent anticardiolipin antibodies (β2GPI-dependent aCL; autoimmune type) can predict an adverse pregnancy outcome in healthy pregnant women and β2GPI-dependent aCL should be applied for routine screening of the population. A prospective cohort study performed on 1600 from whom blood samples were obtained at about week 10 gestation. We used a modified enzyme-linked immunosorbent assay with which divide subjects into three...
Objective Thromboembolic manifestations or thrombocytopenia in association with anticardiolipin antibodies (aCL) lupus anticoagulant are known as the antiphospholipid syndrome (APS). Efforts have been made to elucidate precise clinical features and adequate therapeutic options for treating patients APS. However, lack of a proper international standard measurement aCL makes it difficult compare data derived from different laboratories. We attempted design chimeric antibody human γ constant...