A5049164194- RNA Interference and Gene Delivery
- Synthetic Organic Chemistry Methods
- Advanced Chemical Sensor Technologies
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Chemical Synthesis and Analysis
- Asymmetric Synthesis and Catalysis
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Crystallography and molecular interactions
- Gas Sensing Nanomaterials and Sensors
- Analytical Chemistry and Chromatography
- Chemical Synthesis and Reactions
- Insect Pheromone Research and Control
- Mass Spectrometry Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Liver Diseases and Immunity
- Catalytic Alkyne Reactions
- Metabolomics and Mass Spectrometry Studies
- Cyclization and Aryne Chemistry
- Oxidative Organic Chemistry Reactions
- Cystic Fibrosis Research Advances
- Synthesis and Biological Evaluation
- Fluorine in Organic Chemistry
- Mitochondrial Function and Pathology
University of Louisville
2016-2025
University of Louisville Hospital
2010-2022
Kentucky Science Center
2015-2020
U.S. National Science Foundation
2019
Diabetes Australia
2019
American Heart Association
2017-2018
University of California, Davis
1998-2014
University of Pennsylvania
2010
University of California Davis Medical Center
1997-2009
American University of Beirut
2005
Acetaldehyde, acrolein, and formaldehyde are the principal toxic aldehydes present in cigarette smoke contribute to risk of cardiovascular disease noncancerous pulmonary disease. The rapid growth use electronic cigarettes (e-cigarettes) has raised concerns over emissions these harmful aldehydes. This work determines both free bound (aldehyde–hemiacetal) forms other carbonyls from e-cigarettes. A novel silicon microreactor with a coating phase 4-(2-aminooxyethyl)-morpholin-4-ium chloride...
Infectious and environmental agents have been proposed as immunologic triggers for primary biliary cirrhosis (PBC). Recently, a ubiquitous organism that metabolizes organic compounds estrogens, Novosphingobium aromaticivorans , has defined. Importantly, 2 bacterial proteins homology with the E2 component of pyruvate dehydrogenase complex (PDC–E2). Sera from 97 patients PBC, 46 first–degree relatives, 10 spouses, 195 controls were studied reactivity against N. Escherichia coli . The was...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of bryostatin 7Masanori Kageyama, Tadashi Tamura, Michael H. Nantz, John C. Roberts, Peter Somfai, David Whritenour, and Satoru MasamuneCite this: J. Am. Chem. Soc. 1990, 112, 20, 7407–7408Publication Date (Print):September 1, 1990Publication History Published online1 May 2002Published inissue 1 September 1990https://pubs.acs.org/doi/10.1021/ja00176a058https://doi.org/10.1021/ja00176a058research-articleACS PublicationsRequest reuse...
The flavonoid genistein and the benzo[c]quinolizinium MPB-07 have been shown to activate cystic fibrosis transmembrane conductance regulator (CFTR), protein that is defective in fibrosis. Lead-based combinatorial parallel synthesis yielded 223 flavonoid, quinolizinium, related heterocyclic compounds. compounds were screened for their ability CFTR at 50 μmconcentration by measurement of kinetics iodide influx Fisher rat thyroid cells expressing wild-type or G551D together with green...
Abstract Emerging evidence has suggested environmental factors as causative agents in the pathogenesis of primary biliary cirrhosis (PBC). We have hypothesized that PBC lipoyl domain immunodominant E2 component pyruvate dehydrogenase (PDC-E2) is replaced by a chemical xenobiotic mimic, which sufficient to break self-tolerance. To address this hypothesis, based upon our quantitative structure-activity relationship data, total 107 potential mimics were coupled lysine residue 15 amino acid...
Early detection of lung cancer is a key factor for increasing the survival rates patients. The analysis exhaled breath promising as noninvasive diagnostic tool diagnosis cancer. We demonstrate quantitative carbonyl volatile organic compounds (VOCs) and identification VOC markers in using unique silicon microreactor technology. consists thousands micropillars coated with an ammonium aminooxy salt capture VOCs by means oximation reactions. Captured aminooxy-VOC adducts are analyzed...
Electronic cigarettes (e-cigarette) have emerged as a popular electronic nicotine delivery system (ENDS) in the last decade. Despite absence of combustion products and toxins such carbon monoxide (CO) tobacco-specific nitrosamines (TSNA), carbonyls including short-chain, toxic aldehydes been detected e-cigarette-derived aerosols up to levels found tobacco smoke. Given health concerns regarding exposures aldehydes, understanding both aldehyde generation e-cigarette exposure is critical. Thus,...
Abstract In primary biliary cirrhosis (PBC), the major autoepitope recognized by both T and B cells is inner lipoyl domain of E2 component pyruvate dehydrogenase. To address hypothesis that PBC induced xenobiotic exposure, we took advantage ab initio quantum chemistry synthesized dehydrogenase, replacing lipoic acid moiety with synthetic structures designed to mimic a xenobiotically modified hapten, quantitated reactivity these sera from patients. Interestingly, antimitochondrial Abs all...
Dual-mode electron paramagnetic resonance (EPR), in which an oscillating magnetic field is alternately applied parallel or perpendicular to the static field, a valuable technique for studying both half-integer and integer spin systems particularly useful transition metals involved redox chemistry. We have this characterization of Mn(III) salen (salen = N,N'-ethylene bis(salicylideneaminato)) complex [(R,R)-(−)-N,N'-bis(3,5-di-tert-butylsalicylidene)-1,2-cyclohexanediaminomanganese(III)],...
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel cause fibrosis. The ΔF508 mutation produces defects gating and cellular processing, whereas G551D primarily a defect. To identify correctors of gating, 50,000 diverse small molecules were screened at 2.5 μM (with forskolin, 20 μM) by an iodide uptake assay epithelial cells coexpressing ΔF508-CFTR fluorescent halide indicator (yellow protein-H148Q/I152L) after rescue 24-h culture 27°C. Secondary...
Abstract The E2 subunit of pyruvate dehydrogenase complex (PDC-E2) is the major autoantigen recognized by antimitochondrial Abs (AMA) in primary biliary cirrhosis (PBC). Recently, we replaced lipoic acid moiety PDC-E2 with a battery synthetic structures designed to mimic xenobiotically modified lipoyl hapten on 12-aa peptide that was found within immunodominant autoepitope and demonstrated AMA PBC reacted against several organic mimotopes as well as, or sometimes significantly better than,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of bryostatins. 1. Construction the C(1)-C(16) fragmentMary A. Blanchette, Michael S. Malamas, H. Nantz, John C. Roberts, Peter Somfai, David Whritenour, Satoru Masamune, Masanori Kageyama, and Tadashi TamuraCite this: J. Org. Chem. 1989, 54, 12, 2817–2825Publication Date (Print):June 1, 1989Publication History Published online1 May 2002Published inissue 1 June...
Curcumin is known to have immense therapeutic potential but hindered by poor solubility and rapid degradation in solution. To overcome these shortcomings, curcumin has been conjugated chitosan through a pendant glutaric anhydride linker using amide bond coupling chemistry. The hybrid polymer characterized UV–visible, fluorescence, infrared spectroscopies as well zeta measurements SEM imaging. conjugation reactivity was confirmed gel permeation chromatography quantification of unconjugated...
Hepatic ischemia/reperfusion (I/R) injury is characterized by the generation of reactive oxygen species (ROS), such as superoxide anions and hydrogen peroxide. The aim this study to investigate whether antioxidative gene delivery our polylipid nanoparticles (PLNP) an effective approach for prevention injury. Polyplexes extracellular dismutase (EC-SOD) and/or catalase genes were injected via portal vein 1 day prior a warm I/R procedure in mice. effects determined 6 hours after starting...
A panel of lipidic tetraalkylammonium chlorides has been prepared and screened in studies both lipid hydration vivo mouse transfection. The effect cationic structure on liposome surface was determined using differential scanning calorimetry. Increases headgroup steric bulk the inclusion cis-unsaturation hydrophobic domain led to greater hydration, indicative a decrease polar associations. Cationic lipids containing hydrogen-bonding functionality exhibited corresponding observed an increase...
This paper describes a procedure for direct conversion of aldehydes to nitriles using O-(diphenylphosphinyl)hydroxylamine (DPPH). Aldehydes are smoothly transformed their corresponding by heating with DPPH in toluene. The reaction can be accomplished the presence alcohol, ketone, ester, or amine functionality.
Analysis of volatile organic compounds (VOCs) in exhaled breath (EB) has shown great potential for disease detection including lung cancer, infectious respiratory diseases, and chronic obstructive pulmonary disease. Although many sample collection analytical methods have been developed analysis, analysis metabolic VOCs is still a challenge clinical application. Many carbonyl are related to the processes diseases. This work reports method ultrahigh-performance liquid chromatography coupled...