A5067167249- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Geological and Geochemical Analysis
- Thin-Film Transistor Technologies
- Geology and Paleoclimatology Research
- earthquake and tectonic studies
- Analog and Mixed-Signal Circuit Design
- Cancer-related gene regulation
- Enzyme Catalysis and Immobilization
- Photochromic and Fluorescence Chemistry
- Radio Frequency Integrated Circuit Design
- Advanced DC-DC Converters
- Nanoplatforms for cancer theranostics
- Low-power high-performance VLSI design
- MicroRNA in disease regulation
- Advanced Surface Polishing Techniques
- Earthquake and Disaster Impact Studies
- DNA and Nucleic Acid Chemistry
- Particle Dynamics in Fluid Flows
- Seismology and Earthquake Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrohydrodynamics and Fluid Dynamics
Okayama University
2016-2025
Semiconductor Energy Laboratory (Japan)
2012-2024
Hokkaido University
1976-2022
Japanese Foundation For Cancer Research
2021-2022
Advanced Institute of Industrial Technology
2022
Hoshi University
2017
Ehime University
2003-2015
Keio University
2014-2015
Central Research Institute of Electric Power Industry
1996-2014
The University of Tokyo
1982-2014
Digital LDO is proposed to provide the low noise and tunable power supply voltage 0.5-V near-threshold logic circuits. Because conventional feedback-controlled by operational amplifier fail operate at 0.5V, digital eliminates all analog circuits controlled circuits, which enables operation. The developed in 65nm CMOS achieved input 0.45-V output with 98.7% current efficiency 2.7-μA quiescent 200-μA load current. Both are lowest values published LDO's, indicates good energy of
Boron (B) neutron capture therapy (BNCT) is a novel non-invasive targeted cancer based on the nuclear reaction 10B (n, alpha) 7Li that enables death of cells without damaging neighboring normal cells. However, development clinically approved boron drugs remains challenging. We have previously reported self-forming nanoparticles for drug delivery consisting biodegradable polymer, namely, “AB-type” Lactosome® (AB-Lac particles)- highly loaded with hydrophobic B compounds, namely o-Carborane...
By fractionation using polyacrylamide gel electrophoresis and/or a preparative hybrid selection method employing solid-phase DNA probes, we prepared and characterized mitochondrial tRNAs from the body wall muscle of Ascaris suum, all which are thought to lack either T stem or D their gene sequences (Okimoto, R., Wolstenholme, D. R. (1990) EMBO J. 10, 3405-3411). Some partially purified were appreciably aminoacylated with an extract A. suum mitochondria. The three species sequenced had CCA...
This paper presents a 0.6 V input, 0.3-0.55 output buck converter in 40 nm CMOS, for low-voltage low-power wireless sensor network systems. A low power CCM/DCM controller of the enables automatic selection DCM or CCM operation depending on load situation, therefore improving efficiency. dual-mode-body-biased (DMBB) zero-crossing detector with both forward body bias mode and zero is designed to enable supply voltage normal voltage. An ultra-low-power hysteresis proposed modes selection. The...
In many drug delivery strategies, an inefficient transfer of macromolecules such as proteins and nucleic acids to the cytosol often occurs because their endosomal entrapment. One methods overcome this problem is photochemical internalization, which achieved using a photosensitizer light facilitate escape macromolecule. study, we examined molecular mechanism internalization cell penetrating peptide-cargo (macromolecule)-photosensitizer conjugates. We measured photophysical properties eight...
Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from cytoplasm to nucleus have been studied in yeast, rat hepatoma human cells. In current study, we investigated HeLa cells under various forms stress. We found initiator tRNA(Met) (tRNA(iMet)) was specifically degraded heat Two exonucleases, Xrn1 Xrn2, are involved tRNA(iMet) nucleus, respectively. addition degradation, observed nucleus. also mammalian target rapamycin (mTOR), which regulates...
Pancreatic cancer is one of the cancers with very poor prognosis; there an urgent need to identify novel biomarkers improve its clinical outcomes. Circulating tumor DNA (ctDNA) from liquid biopsy has arisen as a promising biomarker for detection and surveillance. However, it known that ctDNA rate in resected pancreatic low compared other types cancer. In this study, we collected paired plasma samples 145 patients. Plasma were 71 patients treatment-naïve status 74 after neoadjuvant therapy...
Transfer RNA (Gm18) methyltransferase (TrmH (SpoU)) catalyzes the transfer of a methyl group from S-adenosyl-l-methionine (AdoMet) to 2'-OH guanosine 18 in tRNA. This enzyme is member SpoU family methyltransferases. Recent computational researches have shown that three amino acid sequence motifs are conserved among members. Recently, we determined crystal structures apoand AdoMet bound forms TrmH (Nureki, O., Watanabe, K., Fukai, S., Ishii, R., Endo, Y., Hori, H., and Yokoyama, S. (2004)...
The step coverage of submicron trenches by silica films formed low‐pressure chemical vapor deposition is studied, using a novel approach called the "micro/macrocavity method" that analyzes simultaneously growth rate profiles at millimeter and scales. A mechanism strongly indicated in which quality governed diffusion sticking coefficient only one reaction intermediate, produced from silane oxygen gas phase. experimentally correlated with temperature, shape, size trench, reasonably interpreted...
A novel continuous-flow scCO(2) process for kinetic resolution of racemic alcohols can be performed with an immobilized lipase to lead a quantitative mixture the corresponding optically active acetates up 99% ee and unreacted ee, in which productivity compounds was improved by over 400 times compared batch reaction using scCO(2).
An S-adenosylmethionine-dependent tRNA (guanosine-2'-)-methyltransferase (EC 2.1.1.34)was purified to the homogeneous state (2,400-fold) from a cell-free extract of an extreme thermophile, Thermus thermphilus HB27.The enzyme was highly resistant heat as reported €or other enzymes thermophilic organisms.The i s monomeric and its molecular weight estimated be about 20,000.The K , values S-adenosylmethionine Escherichia coli tRNAphe were determined 0.47 PM 10 n ~, respectively, while Kt for...
The contents of 2'-O-methylguanosine and 1-methyladenosine in unfractionated tRNA obtained from Thermus thermophilus HB27 were found to increase significantly when the bacterium was grown at a higher temperature (80 degrees C). S-Adenosyl-L-methionine-dependent (guanosine-2')-methyltransferase (EC 2.1.1.34) (adenine-1)-methyltransferase 2.1.1.36) detected cell-free extract thermophile, both them partially purified. specifically catalyzed methylation guanylate residue position 19 5' end...
A conserved guanosine at position 18 (G18) in the D-loop of tRNAs is often modified to 2'-O-methylguanosine (Gm). Formation Gm18 eubacterial tRNA catalyzed by (Gm18) methyltransferase (TrmH). TrmH enzymes can be divided into two types based on their substrate specificity. Type I TrmH, including Thermus thermophilus modify all species, whereas type II for example Escherichia coli modifies only a subset species. Our previous crystal study showed that T. class IV...
Abstract Magnetic anisotropies of ferromagnetic thin films are induced by epitaxial strain from the substrate via strain-induced anisotropy in orbital magnetic moment and that spatial distribution spin-polarized electrons. However, preferential occupation metallic La 1− x Sr MnO 3 (LSMO) studied x-ray linear dichroism (XLD) has always been found out-of-plane for both tensile compressive hence irrespective anisotropy. In order to resolve this mystery, we directly probed electrons LSMO under...