A5038399358- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Nitric Oxide and Endothelin Effects
- Peptidase Inhibition and Analysis
- Protein Kinase Regulation and GTPase Signaling
- Biochemical and Molecular Research
- PI3K/AKT/mTOR signaling in cancer
- Metabolism, Diabetes, and Cancer
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Porphyrin Metabolism and Disorders
- Polyamine Metabolism and Applications
- Protein Tyrosine Phosphatases
- Enzyme Structure and Function
- Antibiotics Pharmacokinetics and Efficacy
- Metalloenzymes and iron-sulfur proteins
- Carbohydrate Chemistry and Synthesis
- Advanced biosensing and bioanalysis techniques
- Redox biology and oxidative stress
- Gout, Hyperuricemia, Uric Acid
- Diet, Metabolism, and Disease
Ehime University
2016-2025
Gifu Prefecture General Education Center
2021
Kwansei Gakuin University
2020
EQT (United States)
2020
Ministry of Health Labour and Welfare
2009-2019
Gifu University
2015
Nihon University
2015
National Center For Child Health and Development
2015
The University of Tokyo
1989-2013
University of California, Berkeley
2012
Heme-binding protein 23 kDa (HBP23), a rat isoform of human proliferation-associated gene product (PAG), is member the peroxiredoxin family peroxidases, having two conserved cysteine residues. Recent biochemical studies have shown that HBP23/PAG an oxidative stress-induced and proliferation-coupled multifunctional exhibits specific bindings to c-Abl tyrosine kinase heme, as well peroxidase activity. A 2.6-A resolution crystal structure HBP23 in oxidized form revealed unusual dimer which...
Dengue virus is an enveloped positive-strand RNA with a genome approximately 11 kilobases in length. The four serotypes of dengue are currently the most important members flavivirus family terms geographical distribution and incidence infection humans. In this communication we describe successful cloning stable full-length cDNA copy type 4 that can be used as template for vitro transcription infectious RNA. Evidence presented recovered from permissive cells transfected transcripts retained...
Foreign RNA serves as pathogen-associated molecular pattern (PAMP) and is a potent immune stimulator for innate receptors. However, the role of single bacterial species in activation has not been characterized detail. We analyzed immunostimulatory potential transfer (tRNA) from different bacteria. Interestingly, tRNA induced type I interferon (IFN) inflammatory cytokines mouse dendritic cells (DCs) human peripheral blood mononuclear (PBMCs). Cytokine production was TLR7 dependent because...
Phosphatidylinositol (PI) 3-kinase plays an important role in various metabolic actions of insulin including glucose uptake and glycogen synthesis. Although PI primarily functions as a lipid kinase which preferentially phosphorylates the D-3 position phospholipids, effect hydrolysis key product 3,4,5-triphosphate [PI(3,4,5)P3] on these biological responses is unknown. We recently cloned rat SH2-containing inositol phosphatase 2 (SHIP2) cDNA possesses 5′-phosphatase activity to hydrolyze...
Mammalian xanthine dehydrogenase can be converted to oxidase by modification of cysteine residues or proteolysis the enzyme polypeptide chain. Here we present evidence that Cys535 and Cys992 rat liver are indeed involved in rapid conversion from oxidase. The purified mutants C535A and/or C992R were significantly resistant incubation with 4,4′-dithiodipyridine, whereas recombinant wild-type readily type, indicating these responsible for conversion. C535A/C992R mutant, however, very slowly...
N7-methylguanine at position 46 (m7G46) in tRNA is produced by methyltransferase (TrmB). To clarify the role of this modification, we made a trmB gene disruptant (ΔtrmB) Thermus thermophilus, an extreme thermophilic eubacterium. The absence TrmB activity cell extract from ΔtrmB strain and lack m7G46 modification tRNAPhe were confirmed enzyme assay, nucleoside analysis RNA sequencing. When was cultured high temperatures, several modified nucleotides hypo-modified addition to modification....
Pseudouridine at position 55 (Ψ55) in eubacterial tRNA is produced by TruB. To clarify the role of Ψ55 modification, we constructed a truB gene disruptant (Δ ) strain Thermus thermophilus which an extreme-thermophilic eubacterium. Unexpectedly, Δ exhibited severe growth retardation 50°C. We assumed that these phenomena might be caused lack RNA chaperone activity TruB, was previously hypothetically proposed others. confirm this idea, replaced genome with mutant genes, express TruB proteins...
SH-2-containing inositol 5′-phosphatase 2 (SHIP-2) is a physiologically important lipid phosphatase that functions to hydrolyze phosphatidylinositol (PI) 3-kinase product PI(3,4,5)P3 PI(3,4)P2 in the negative regulation of insulin signaling. We investigated whether SHIP-2 associated with resistance diabetic db/db mice. The amount protein was elevated quadriceps muscle and epididymal fat tissue, but not liver, mice relative control db/+m In accordance enhanced expression SHIP-2, its...
Rat heme-binding protein 23 (HBP23)/peroxiredoxin (Prx I) belongs to the 2-Cys peroxiredoxin type I family and exhibits peroxidase activity coupled with reduced thioredoxin (Trx) as an electron donor. We analyzed dimer-oligomer interconversion of wild-type mutant HBP23/Prx by gel filtration found that C52S C173S mutants existed mostly decamers, whereas wild was a mixture various forms, favoring decamer at higher concentration lower ionic salt in presence dithiothreitol. The C83S...
tRNAs from all 3 phylogenetic domains have a 5-methyluridine at position 54 (T54) in the T-loop. The methyl group is transferred S-adenosylmethionine by TrmA methyltransferase most Gram-negative bacteria and some archaea eukaryotes, whereas it 5,10-methylenetetrahydrofolate (MTHF) TrmFO, folate/FAD-dependent methyltransferase, Gram-positive bacteria. However, catalytic mechanism remains unclear, because crystal structure of TrmFO has not been solved. Here, we report structures Thermus...
Transfer RNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes N2,N2-dimethylguanine formation at position 26 (m22G26) in tRNA. In the reaction, N2-guanine (m2G26) is generated as an intermediate. The trm1 genes are found only archaea and eukaryotes, although it has been reported that Aquifex aeolicus, a hyper-thermophilic eubacterium, putative gene. To confirm whether A. aeolicus Trm1 tRNA methyltransferase activity, we purified recombinant protein. vitro methyl transfer assay revealed...
Stress response mechanisms that modulate the dynamics of tRNA degradation and accumulation from cytoplasm to nucleus have been studied in yeast, rat hepatoma human cells. In current study, we investigated HeLa cells under various forms stress. We found initiator tRNA(Met) (tRNA(iMet)) was specifically degraded heat Two exonucleases, Xrn1 Xrn2, are involved tRNA(iMet) nucleus, respectively. addition degradation, observed nucleus. also mammalian target rapamycin (mTOR), which regulates...
As demonstrated by the United Nations High-Level Meeting on tuberculosis (TB) held in September 2018, political momentum for TB has been increasing. The aim of this study was to analyze current challenges and opportunities global control and, with specific focus policies surrounding control, reveal what kinds efforts are needed accelerate control.We organized two expert meetings purposes assessing situation analyzing regarding control. By applying Shiffman Smith's framework which contains...
Transfer RNA (Gm18) methyltransferase (TrmH (SpoU)) catalyzes the transfer of a methyl group from S-adenosyl-l-methionine (AdoMet) to 2'-OH guanosine 18 in tRNA. This enzyme is member SpoU family methyltransferases. Recent computational researches have shown that three amino acid sequence motifs are conserved among members. Recently, we determined crystal structures apoand AdoMet bound forms TrmH (Nureki, O., Watanabe, K., Fukai, S., Ishii, R., Endo, Y., Hori, H., and Yokoyama, S. (2004)...