A5056761543- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Adipose Tissue and Metabolism
- Advanced Cellulose Research Studies
- Paraoxonase enzyme and polymorphisms
- Metabolism, Diabetes, and Cancer
- HIV/AIDS Research and Interventions
- Pancreatic function and diabetes
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Peptidase Inhibition and Analysis
- Genomics and Phylogenetic Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Cytomegalovirus and herpesvirus research
- Metabolism and Genetic Disorders
- Hepatitis C virus research
- Glycosylation and Glycoproteins Research
- Protein Kinase Regulation and GTPase Signaling
- Diabetes and associated disorders
- Infective Endocarditis Diagnosis and Management
- Cholesterol and Lipid Metabolism
- Hepatitis B Virus Studies
- Mitochondrial Function and Pathology
Showa University Northern Yokohama Hospital
2006-2025
Kitakyushu City Hospital Organization
2025
Juntendo University Hospital
2019-2025
Nurix (United States)
2025
The University of Tokyo
2011-2024
Brigham and Women's Hospital
2015-2024
Harvard University
2015-2024
Howard Hughes Medical Institute
2015-2024
Cornell University
2024
New York State College of Veterinary Medicine
2024
Never-dried and once-dried hardwood celluloses were oxidized by a 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated system, highly crystalline individualized cellulose nanofibers, dispersed in water, prepared mechanical treatment of the cellulose/water slurries. When carboxylate contents formed from primary hydroxyl groups reached approximately 1.5 mmol/g, slurries mostly converted to transparent viscous dispersions treatment. Transmission electron microscopic observation showed...
Adiponectin is an adipocyte-derived hormone, which has been shown to play important roles in the regulation of glucose and lipid metabolism. Eight mutations human adiponectin have reported, some were significantly related diabetes hypoadiponectinemia, but molecular mechanisms decreased plasma levels impaired action mutants not clarified. structurally belongs complement 1q family known form a characteristic homomultimer. Herein, we demonstrated that simple SDS-PAGE under non-reducing...
The adipocyte-derived hormone adiponectin has been shown to play important roles in the regulation of energy homeostasis and insulin sensitivity. In this study, we analyzed globular domain (gAd) transgenic (Tg) mice crossed with leptin-deficient ob/ob or apoE-deficient mice. Interestingly, despite an unexpected similar body weight, gAd Tg showed amelioration resistance β-cell degranulation as well diabetes, indicating that leptin appeared have both distinct overlapping functions....
A new catalytic oxidation using 2,2,6,6-tetramethylpiperidinyl-1-oxyl (TEMPO) and NaClO is applied to hardwood cellulose in water at 60 °C pH 6.8 with NaClO2 used as a primary oxidant. The oxidized celluloses carboxylate content of approximately 0.8 mmol/g were convertible highly crystalline individual fibrils 5 nm width least 2 μm length by disintegration water. had no aldehyde groups, high degrees polymerization more than 900. Solid-state 13C NMR X-ray analyses revealed that the C6 groups...
Peroxisome proliferator-activated receptor (PPAR) γ is a ligand-activated transcription factor and member of the nuclear hormone superfamily that thought to be master regulator fat storage; however, relationship between PPARγ insulin sensitivity highly controversial. We show here supraphysiological activation by agonist thiazolidinediones (TZD) markedly increases triglyceride (TG) content white adipose tissue (WAT), thereby decreasing TG liver muscle, leading amelioration resistance at...
AbstractIn an attempt to identify transcription factors which activate sterol-regulatory element-binding protein 1c (SREBP-1c) transcription, we screened expression cDNA library from adipose tissue of SREBP-1 knockout mice using a reporter gene containing the 2.6-kb mouse promoter. We cloned and identified oxysterol receptors liver X receptor (LXRα) LXRβ as strong activators SREBP-1c In transfection studies, either LXRα or -β activated promoter-luciferase in dose-dependent manner. Deletion...
To investigate the role of insulin receptor substrate (IRS)-2 in vivo, we generated IRS-2-deficient mice by gene targeting. Although homozygous (IRS-2-/- mice) had a body weight similar to wild-type mice, they progressively developed type 2 diabetes at 10 weeks. IRS-2-/- showed resistance and defect insulin-stimulated signaling pathway liver but not skeletal muscle. Despite resistance, amount beta-cells was reduced 83% that which marked contrast 85% increase IRS-1-deficient (IRS-1-/-...
Recent studies on the in vivo roles of sterol regulatory element binding protein (SREBP) family indicate that SREBP-2 is more specific to cholesterogenic gene expression whereas SREBP-1 targets lipogenic genes. To define molecular mechanism involved this differential regulation, luciferase-reporter assays were performed HepG2 cells compare transactivities nuclear SREBP-1a, -1c, and -2 a battery SREBP-target promoters containing (SRE), SRE-like, or E-box sequences. The results show first...
In living organisms, nanoscale building blocks are well organized into a nearly defect-free arrangement, leading to sophisticated hierarchical structures that exhibit high mechanical strengths and functionalities. Cellulose nanofibrils representative in nature have raised great interest as excellent structural materials, originating from the most abundant bioresource, wood biomass. Here, we report integration controls of cellulose been completely dispersed water through specific...
The worldwide prevalence of type 2 diabetes (T2D), which is caused by a combination environmental and genetic factors, increasing. With regard to variations in the gene encoding Cdk5 regulatory associated protein 1-like 1 (Cdkal1) have been with an impaired insulin response increased risk T2D across different ethnic populations, but molecular function this has not characterized. Here, we show that Cdkal1 mammalian methylthiotransferase biosynthesizes...
PPARγ is a ligand-activated transcription factor and functions as heterodimer with retinoid X receptor (RXR). Supraphysiological activation of by thiazolidinediones can reduce insulin resistance hyperglycemia in type 2 diabetes, but these drugs also cause weight gain. Quite unexpectedly, moderate reduction activity observed heterozygous PPARγ-deficient mice or the Pro12Ala polymorphism human PPARγ, has been shown to prevent obesity induced high-fat diet. In this study, we investigated...
We developed a new quantitative system for diagnosis of invasive pulmonary aspergillosis (IPA) using real-time automated polymerase chain reaction (PCR). Intra-assay and interassay precision rates in vitro examination were 2.53% 2.20%, respectively, the linearity this assay was obtained when there >20 copies/well. examined 323 samples taken from 122 patients with hematological malignancies, including 33 IPA 89 control patients. Blood subjected to PCR antigen detection methods, enzyme-linked...
The tumor suppressor p53 is a transcription factor that activates or represses its target genes after various genotoxic stresses. We have previously shown sterol regulatory element-binding protein-1 (SREBP-1), key transcriptional regulator of triglyceride synthesis, and the lipogenic enzymes under control are markedly suppressed in adipocytes from genetically obese ob/ob mice. Here we demonstrate highly induced mice fed state, leading to negative regulation SREBP-1 thereby genes. In fact,...
AbstractTo investigate the role of insulin receptor substrate 1 (IRS-1) and IRS-2, two ubiquitously expressed IRS proteins, in adipocyte differentiation, we established embryonic fibroblast cells with four different genotypes, i.e., wild-type, IRS-1 deficient (IRS-1−/−), IRS-2 (IRS-2−/−), double (IRS-1−/−IRS-2−/−), from mouse embryos corresponding genotypes. The abilities IRS-1−/− IRS-2−/− to differentiate into adipocytes are approximately 60 15%, respectively, lower than that wild-type...
Advanced glycation end products (AGEs) are nonenzymatically glycosylated proteins, which accumulate in vascular tissues aging and diabetes. Receptors for AGEs include scavenger receptors, recognize acetylated low density lipoproteins (Ac-LDL) such as receptor class AI/AII (SR-A), cell surface glycoprotein CD36, B type I (SR-BI), lectin-like oxidized lipoprotein receptor-1. The broad ligand repertoire of these receptors well the diversity have prompted us to examine whether also recognized by...
In bacterial 16S rRNAs, methylated nucleosides are clustered within the decoding center, and these nucleoside modifications thought to modulate translational fidelity. The N4, 2′-O-dimethylcytidine (m4Cm) at position 1402 of Escherichia coli rRNA directly interacts with P-site codon mRNA. biogenesis function this modification remain unclear. We have identified two previously uncharacterized genes in E. that required for m4Cm formation. mraW (renamed rsmH) yraL rsmI) encode methyltransferases...