A5078107192- Gut microbiota and health
- Probiotics and Fermented Foods
- Gastrointestinal motility and disorders
- Diet and metabolism studies
- Clostridium difficile and Clostridium perfringens research
- Liver Disease Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolites in Food Biotechnology
- Viral gastroenteritis research and epidemiology
- Oral health in cancer treatment
- Nutritional Studies and Diet
- Pancreatitis Pathology and Treatment
- Genomics and Phylogenetic Studies
- Pancreatic and Hepatic Oncology Research
- Cardiovascular Function and Risk Factors
- Microbial Metabolism and Applications
- Neutropenia and Cancer Infections
- Barrier Structure and Function Studies
- Agriculture Sustainability and Environmental Impact
- Parasitic Infections and Diagnostics
- Pharmacy and Medical Practices
- Spinal Hematomas and Complications
- Infant Health and Development
- Infant Nutrition and Health
- Nutrition, Health and Food Behavior
Senju Pharmaceutical (Japan)
2020-2024
Japan Biological Informatics Consortium
2021
Kyoto Pharmaceutical University
2006-2019
RIKEN Nishina Center
2014-2015
Kobe Asahi Hospital
2012-2015
RIKEN
2013-2014
Imperial Household Agency
1990
Tohoku University
1989
Kansai Medical University
1986-1988
Validation and standardization of methodologies for microbial community measurements by high-throughput sequencing are needed to support human microbiome research its industrialization. This study set out establish standards-based solutions improve the accuracy reproducibility metagenomics-based profiling fecal samples.
Although gut microbiota and early life events are likely involved in the development of irritable bowel syndrome (IBS), it remains unclear how these factors interact pathophysiology IBS. In present study, using rats subjected to maternal separation (MS) as a model IBS, we investigated interrelationships among microbiota, stress susceptibility intestinal permeability, examined effect probiotic Bifidobacterium bifidum G9-1 (BBG9-1) on those interrelationships. When compared with controls at...
A treatment option for constipation that improves the quality of life is needed since available laxatives do not effectively improve in patients with constipation. significant association between gut dysbiosis and recognized, suggesting probiotics may be an important management The underlying mechanism by which remains unclear. In this study, we aimed to evaluate effects probiotic Bifidobacterium bifidum G9-1 (BBG9-1) on loperamide-induced delayed colonic transit elucidate its action. First,...
Fluoropyrimidines (FPs) are key drugs in many chemotherapy regimens; however, recipients often prone to diarrhea due gastrointestinal toxicity. Disruption of the intestinal epithelial barrier function by FPs leads dysbiosis, which may exacerbate cell damage as a secondary effect and trigger diarrhea. However, despite studies on chemotherapy-induced changes microbiome humans, relationship between dysbiosis is unclear. In this study, we aimed investigate microbiome.We conducted single-center...
Two bacterial strains, designated 180-3(T) and 214-4(T), isolated from human faeces were characterized by using a polyphasic taxonomic approach that included analysis of their phenotypic biochemical features, cellular fatty acid profiles, menaquinone profiles phylogenetic positions based on 16S rRNA gene sequence analysis. showed these strains represented members the genus Butyricimonas. These shared 97.9 % similarity with each other related to Butyricimonas virosa JCM 15149(T) (97...
Bifidobacterium, a major component of the intestinal microbiota, has been clinically used for treatment diarrhoea and constipation. 5-Fluorouracil (5-FU), widely cancer chemotherapy, is known to frequently induce mucositis accompanied by severe diarrhoea. The present study examined effect Bifidobacterium bifidum G9-1 (BBG9-1) on 5-FU-induced in mice. Intestinal was induced repeated administration 5-FU 6 days. BBG9-1 administered orally once daily 9 days, beginning 3 days before onset...
Background Proton pump inhibitors (PPIs) can alleviate upper gastrointestinal injury but paradoxically exacerbate aspirin (ASA)-induced small intestine injury. In this study, our goal was to simulate exacerbation by developing an appropriate animal model, which may help in establishing treatments. Methods: Male mice were fed a 60% fructose diet for 9 weeks, then administered 200 mg/kg ASA 3 h before sacrifice. The PPI omeprazole intraperitoneally once daily weeks. Bifidobacterium bifidum...
Human rotavirus (RV) infection is a leading cause of dehydrating diarrhea in infants and young children worldwide. Since therapeutic approaches to RV gastroenteritis are limited alleviation dehydration with oral rehydration solutions, more direct palliate symptoms required. Treatments probiotics have been increasingly recognized as alternative safe low cost treatments for moderate infectious diarrhea. In this study, Bifidobacterium bifidum G9-1 (BBG9-1), which has used an intestinal drug...
Metformin is reported to affect human gut microbiota; however, the nature of this association in Japanese patients with type 2 diabetes mellitus (T2DM) unknown. We enrolled 31 T2DM who took metformin for first time study. compared them before and after four weeks taking metformin. Fecal samples were collected 16S rDNA sequences performed identify microbiota. Blood Gastrointestinal Symptom Rating Scale (GSRS) questionnaire results, denoting gastro-intestinal symptoms, also collected. In...
Gut microbiota composition is associated with the pathogenesis of non-alcoholic fatty liver disease. However, association between gut and disease in non-obese patients remains unclear. We compared clinical parameters profiles healthy controls obese disease.We examined by 16S rRNA sequences short-chain acid levels fecal samples from 51 (body mass index <25 kg/m2 ) ≥30 who underwent pathological examination 87 at five hospitals Japan.Although no significant differences other groups were...
Although high-fat diet (HFD)-related dysbiosis is involved in the development of steatohepatitis, its pathophysiology especially small intestine remains unclear. We comprehensively investigated not only liver pathology but also microbiome profile, mucosal integrity and luminal environment mice with HFD-induced obesity. C57BL/6J were fed either a normal or an HFD, their small-intestinal contents subjected to microbial 16S rDNA analysis. Intestinal permeability was evaluated by FITC-dextran...
The aim of this research was to reveal the characteristics gut microbiome altered by acarbose intervention in Japanese patients with type 2 diabetes (T2D) and its possible association habitual dietary intake. Eighteen T2D were administered for four weeks. abundances two major phyla, namely Actinobacteria Bacteroidetes, reciprocally changed accompanied intervention. There also significant changes ten genera, including greater abundance Bifidobacterium, Eubacterium, Lactobacillus lower...
Molecular studies have led to postulation of a relationship between gut microbiota and certain diseases. However, because hitherto uncultured species in vivo are essential for characterizing the biology pathogenic properties bacteria, techniques culturing isolating such bacteria must be developed. Here, technique is described that partially overcomes obstacles prevent detection interbacterial communication vitro thus responsible failure culture bacterial species. For this purpose, ring with...
Although dysbiosis is likely to disturb the mucosal barrier system, mechanism involved has remained unclear. Here, we investigated alterations of colonic permeability and tight junction (TJ) molecules in mice with antibiotic-induced dysbiosis. Mice were orally administered vancomycin or polymyxin B for 7 days, then fecal samples subjected microbial 16S rRNA analysis. The was evaluated by chamber assay. expression TJ cytokines examined real-time RT-PCR, Western blotting, immunohistochemistry....
ABSTRACT Supplementation with Bifidobacterium bifidum G9‐1 (BBG9‐1) has been established to enhance the production of butyrate, a short‐chain fatty acid (SCFA) known for its beneficial effects in alleviating constipation. We hypothesized that BBG9‐1 alters gut microbiota such bacteria produce butyric from lactate and acetate become more abundant. In this study, we sought determine whether promotes growth butyrate‐producing thereby enhances butyrate production. was cocultured different...
A bacterial strain, designated 157(T), isolated from human faeces was characterized by using a polyphasic taxonomic approach, which included analysis of physiological and biochemical features, cellular fatty acid profiles, menaquinone profiles its phylogenetic position, based on 16S rRNA gene sequence analysis. The strain obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. isolate able to grown medium containing 20% (w/v) bile. showed that the member...
Constipation, a functional disorder of the digestive system, is common in children and adults may compromise patient quality life. Because many patients are not satisfied with efficacy existing therapies, this study, we investigated probiotic Bifidobacterium bifidum G9-1 (BBG9-1) constipation induced by low-fiber diet. After inducing rats feeding diet, were fed diet mixed BBG9-1 14 days to determine for alleviating constipation. significantly alleviated dysbiosis improved fecal counts,...
Although probiotics may be useful for the treatment of irritable bowel syndrome (IBS), it is unclear how play a role in colonic mucosal integrity and immunity. Here, we aimed to investigate effect Bifidobacterium bifidum G9-1 (BBG9-1) on macrophage behavior rats subjected maternal separation (MS) as model IBS. MS pups were individually separated from their mother rats, proportion orally administered BBG9-1. The permeability was evaluated by Ussing chamber assay. expression tight junction...
The intestinal microbiota composition of 92 volunteers living in Japan was identified following the consumption ‘identical meals’ (1,879 kcal/day) for 3 days. When faecal samples were analysed by terminal restriction fragment length polymorphism with several primer-restriction enzyme systems and then clustered, patterns could be divided into 2 clusters. Contribution tests partition modelling showed that OTU211 35f- Msp I system OTU237 Alu key factors distribution these groups. However,...
Recent research has highlighted the importance of gut microbiome in regulating aging, and probiotics are interventions that can promote health. In this study, we surveyed several novel lactic acid bacteria to examine their beneficial effect on organismal health lifespan C. elegans. We found animals fed some bacteria, including L. acidophilus 1244 paracasei subsp. 2004, grew healthy. Supplementation with bacterial strains or 2004 significantly improved health, food consumption, motility,...
The intestinal microbiota compositions of 92 men living in Japan were identified following consumption identical meals for 3 days. Fecal samples analyzed by terminal restriction fragment length polymorphism with 4 primer-restriction enzyme systems, and the 120 obtained operational taxonomic units (OTUs) Data mining software focusing on 5 characteristics, namely, age, body mass index, present smoking habit, cessation period previous smokers drinking according to answers subjects. After...