A5065022467- Esophageal Cancer Research and Treatment
- Esophageal and GI Pathology
- Lung Cancer Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Surgical Treatments
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Colorectal and Anal Carcinomas
- Metastasis and carcinoma case studies
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Tracheal and airway disorders
- CAR-T cell therapy research
- Urinary and Genital Oncology Studies
- Radiomics and Machine Learning in Medical Imaging
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Dysphagia Assessment and Management
- Immune cells in cancer
- Gastrointestinal Tumor Research and Treatment
- Gastrointestinal disorders and treatments
- Lung Cancer Research Studies
- Anorectal Disease Treatments and Outcomes
Akita University
2016-2025
Aichi Cancer Center
2021-2025
Akita University Hospital
2017-2025
Nihon University
2024
Yamagata City Hospital Saiseikan
2023-2024
The University of Tokyo
2012-2024
Nagoya University
2019-2021
RIKEN Center for Integrative Medical Sciences
2013-2019
University of Tokyo Hospital
2016-2019
Oita University
2018
Toll-like receptors (TLRs) play a crucial role in the innate immune response and subsequent induction of adaptive responses against microbial infection or tissue injury. Recent findings show that functional TLRs are expressed not only on cells but also cancer cells. an active carcinogenesis tumor progression during chronic inflammation involves microenvironment. Damage-associated molecular patterns (DAMPs) derived from injured normal epithelial necrotic appear to be present at significant...
Molecular pathways determining the malignant potential of premalignant breast lesions remain unknown. In this study, alterations in DNA methylation levels were monitored during benign, and stages ductal cancer development. To study epigenetic events development, four genomic biomarkers (Methylated-IN-Tumour (MINT)17, MINT31, RARβ2 RASSF1A) shown to represent hypermethylation tumours selected. Laser capture microdissection was employed isolate from lesions, including normal epithelia (n=52),...
Tumors commonly harbor multiple genetic alterations, some of which initiate tumorigenesis. Among these, tumor-specific somatic mutations resulting in mutated protein have the potential to induce antitumor immune responses. To examine relevance latter responses tumor and patient outcomes, we used datasets whole-exome RNA sequencing from 97 clear cell renal carcinoma (ccRCC) patients identify neoepitopes predicted be presented by each patient's autologous HLA molecules. We found that number...
Reprogramming of antigen-specific T lymphocytes into induced pluripotent stem cells (iPSCs) and their subsequent re-differentiation has enabled expansion functional in vitro, thus opening up new approaches for immunotherapy cancer other diseases. In this study, we have established a robust protocol to reprogram human invariant NKT (Vα24+ iNKT) cells, which been shown act as cellular adjuvants exert anti-tumor activity mice humans, re-differentiate the iNKT cell-derived iPSCs cells. These...
Purpose To develop a simple noninvasive technique for evaluating pleural invasion by using routine preoperative computed tomography (CT). Materials and Methods The institutional review board approved this retrospective study, written informed consent was obtained performing the initial follow-up CT studies. Preoperative findings (169 patients with possible invasion) pathologic diagnoses after surgical resection were evaluated. length of interface between primary tumor neighboring structures...
Abstract Esophageal squamous cell carcinoma (ESCC) is one of the most fatal types malignant tumors worldwide. Epitranscriptome, such as N 6 ‐methyladenosine (m A) mRNA, an abundant post‐transcriptional mRNA modification and has been recently implicated to play roles in several cancers, whereas significance m A modifications virtually unknown ESCC. Analysis tissue microarray 177 ESCC patients showed that higher expression demethylase ALKBH5 correlated with poor prognosis was independent...
Background: Immunohistochemistry (IHC) is crucial for determining cancer treatments. We previously developed a rapid IHC method and have now fully automated stainer (R-Auto). This study aimed to evaluate the clinical reliability of R-Auto protocol staining estrogen receptors (ERs) in breast specimens performance. Methods: Between January 2015 June 2020, 188 surgical collected from patients treated at our hospital were evaluated via ER using R-Auto, conventional manual IHC, commercial...
<title>Abstract</title> <bold>Background.</bold> Poor oral health is an independent risk factor for upper-aerodigestive tract cancers, including esophageal squamous cell carcinoma (ESCC). <italic>Porphyromonas gingivalis (P. gingivalis)</italic>, the most studied periodontal pathogen, a Gram-negative bacterium with walls composed of lipopolysaccharide (LPS). Toll-like receptor 4 (TLR4) recognizes LPS, and we previously reported that ESCC patients exhibiting high TLR4 expression also show...
<h3>Abstract</h3> Background/Aim: Comprehensive transcriptome analysis has revealed SPOC Domain Containing 1 (SPOCD1) as a potential biomarker for esophageal squamous cell carcinoma (ESCC). However, the expression and oncological roles of SPOCD1 in ESCC remains underexplored. We aimed to evaluate role oncogenesis prognosis <i>in vitro</i> vivo</i>. Materials Methods: The Cancer Cell Line Encyclopedia (CCLE) database was utilized correlations between oncogenes ESCC. mRNA protein levels were...
Abstract We previously reported that high tumoral expression of Toll-like receptor 3 (TLR3) and CXCL10, a member the CXC chemokine family, was an independent positive prognostic factor in patients with advanced thoracic esophageal squamous cell carcinoma (ESCC). However, direct relationships between TLR3 CXCL10 ESCC cells not fully understood. Here, we analyzed mRNA protein two lines (TE8 KYSE180) one adenocarcinoma (EAC) line (OE19). also assessed effect agonist poly(I:C) on production...
Transforming growth factor (TGF)-β is known to be produced by progressor tumors and immobilize dendritic cells (DCs) within those tumors. Moreover, although TGF-β1 has been shown promote tumor progression, there still no direct, in vivo evidence as whether able directly induce distant metastasis.To address that issue investigate the mechanism which suppresses DC activity, we subdermally inoculated mouse ears with squamous cell carcinoma stably expressing or empty vector (mock).The numbers of...
Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population with the ability to suppress immune responses and currently classified into three distinct MDSC subsets: monocytic, granulocytic non-monocytic, non-granulocytic MDSCs. Although NK provide an important first-line defense against newly transformed cancer cells, it is unknown whether can regulate populations in context of cancer. In this study, we initially found that frequency MDSCs non-Hodgkin lymphoma (NHL) patients was...
Significance Both natural killer (NK) cells and γδT cells, classified as innate immune recently have been shown to features of memory cells. However, after activation, a fate invariant NK T (iNKT cells) has not identified. Here we show the presence effector memory-like KLRG1 + (Killer cell lectin-like receptor subfamily G, member 1–positive) iNKT in lung. The are able recognize respond an antigen context CD1d can persist for long time then mount potent secondary response upon encountering...
Abstract Eomes regulates the differentiation of CD8 + T cells into effector and memory phases. However, its role in invariant (i)NKT remains unknown. Here, we show impact on iNKT thymus peripheral tissue using conditional knockout (Eomes-cKO) mice. In thymus, CD1d-tetramer CD24 CD44 − NK1.1 CD69 stage 0 express higher levels than other stages. We also found that NKT1 cell predominantly. Interestingly, expression steady state is low, but can be upregulated after TCR stimulation. showed...
A key goal of vaccine immunotherapy is the generation long-term memory CD8(+) T cells capable mediating immune surveillance. We discovered a novel intercellular pathway governing development potent cell responses against cell-associated Ags that mediated through cross-presentation by XCR1(+) dendritic (DCs). Generation tumor pulsed with an invariant NKT ligand depended on cross-talk between and plasmacytoid DCs was regulated IFN-α/IFN-αR signals. IFN-α production stimulated OX40 signal from...
Programmed death-1 ligand 1 (PD-L1) induces apoptosis of tumor-reactive T-cells, that enables tumors to evade immune defense and thus furthers their growth. Our aim was determine whether PD-L1 expression status correlates with prognosis in patients advanced thoracic esophageal squamous cell carcinoma.The 177 treated esophagectomy without preoperative therapy evaluated immunohistochemically using tissue microarray. We then statistically analyzed the relationships between clinicopathological...
Loss of skeletal muscle is predictive a poor prognosis in patients with various malignant lesions. Our aim was to determine whether changes after neo-adjuvant therapy (NAT) predict esophageal squamous cell carcinoma (ESCC) undergoing esophagectomy.The cross-sectional areas the psoas muscles were measured on computed tomographic images collected at initial visit, preoperatively and postoperatively 84 patients. The index (PMI) calculated by normalizing patients' heights.Low PMI visit not...
Poor oral health is a risk factor for causing upper aerodigestive tract tumors, including esophageal cancer. Our aim was to determine the periodontitis rate in our cohort of cancer patients. We also analyzed whether preoperative dental examination and care reduces likelihood severe pneumonia after esophagectomy.Between 2003 2014, 529 patients received esophagectomy at Akita University Hospital. studied 232 who had examinations (dental group) retrospectively assessed severity their...
Abstract Strategies to reprogram the tumor microenvironment are being explored improve cancer immunotherapy. In one approach, we have targeted dendritic cells (DC) their function with adjuvant vector (aAVC) that engineered from NKT ligand-loaded CD1d+ allogeneic transfected antigen mRNAs. Here, report finding this approach also programs local immune responses by establishing tertiary lymphoid structures (TLS), which include expanded antigen-specific CD8+ T-cell clones, mobilized DCs, and...
Abstract Background Poor oral health is an independent risk factor for upper-aerodigestive tract cancers, including esophageal cancer. Several studies have investigated short-term outcomes after esophagectomy and the impact of periodontal disease, but few examined disease on long-term outcomes. The purpose this study was to investigate rate periodontitis among patients prognostic value its effect prognosis esophagectomy. Methods A total 508 who underwent received care from a dentist before...