A5029428307- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Cutaneous Melanoma Detection and Management
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Melanoma and MAPK Pathways
- Inflammatory mediators and NSAID effects
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Glioma Diagnosis and Treatment
- Chemokine receptors and signaling
- RNA modifications and cancer
- Sarcoma Diagnosis and Treatment
- Molecular Biology Techniques and Applications
- Bone Tumor Diagnosis and Treatments
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Breast Cancer Treatment Studies
- vaccines and immunoinformatics approaches
Saint John's Health Center
2016-2025
Providence College
2019-2025
St. John's School
2008-2025
Molecular Oncology (United States)
2014-2024
University of Michigan
2022
University of Santa Monica
2011-2019
Lake Macquarie Private Hospital
2018
National Institutes of Health
2015
Cancer Institute (WIA)
2001-2014
Melanoma Institute Australia
2014
Sentinel-lymph-node biopsy is associated with increased melanoma-specific survival (i.e., until death from melanoma) among patients node-positive intermediate-thickness melanomas (1.2 to 3.5 mm). The value of completion lymph-node dissection for sentinel-node metastases not clear.
Post-acute sequelae of COVID-19 (PASC) represent an emerging global crisis. However, quantifiable risk factors for PASC and their biological associations are poorly resolved. We executed a deep multi-omic, longitudinal investigation 309 patients from initial diagnosis to convalescence (2-3 months later), integrated with clinical data patient-reported symptoms. resolved four PASC-anticipating at the time diagnosis: type 2 diabetes, SARS-CoV-2 RNAemia, Epstein-Barr virus viremia, specific...
Next-generation sequencing of cell-free circulating solid tumor DNA addresses two challenges in contemporary cancer care. First this method massively parallel and deep enables assessment a comprehensive panel genomic targets from single sample, second, it obviates the need for repeat invasive tissue biopsies. Digital SequencingTM is novel high-quality simultaneously across over 50 cancer-related genes with simple blood test. Here we report analytic clinical validation gene panel. Analytic...
Relapse to anti-HER2 monoclonal antibody (mAb) therapies, such as trastuzumab in HER2+ breast cancer (BC), is associated with residual disease progression due resistance therapy. Here, we identify interferon-γ inducible protein 16 (IFI16)-dependent STING signaling a significant determinant of responses BC. We show that down-regulation immune-regulated genes (IRG) specifically poor survival HER2+, but not other BC subtypes. Among IRG, IFI16 identified direct target EZH2, the underexpression...
BACKGROUND MicroRNAs (miRs) are a class of small noncoding RNAs whose expression changes have been associated with cancer development and progression. Current techniques to isolate miRs for analysis from blood inefficient. We developed reverse-transcription quantitative real-time PCR (RT-qPCR) assay direct detection circulating in serum. hypothesized that serum concentrations miR-21, biomarker increased breast tumors, would correlate the presence extent cancer. METHODS The RT-qPCR applied...
A new polyvalent melanoma cell vaccine (MCV) was administered to 136 stage. IIIA and IV (American Joint Committee on Cancer) patients. Induction of cell-mediated humoral immune responses common melanoma-associated antigens present autologous cells observed in patients receiving the MCV. This accompanied by increased activation tumor-infiltrating lymphocytes. Survival correlated significantly with delayed cutaneous hypersensitivity (p = 0.0066) antibody MCV 0.0117). Of 40 evaluable disease,...
Liver metastasis is the predominant cause of colorectal cancer (CRC) related mortality. Chemokines, soluble factors that orchestrate hematopoetic cell movement, have been implicated in directing metastasis, although their clinical relevance CRC has not defined. Our hypothesis was chemokine receptor CXCR4 expressed by a prognostic factor for poor disease outcome.CRC lines (n = 6) and tumor specimens 139) from patients with different American Joint Committee on Cancer (AJCC) stages were...
Purpose Cell-free DNA circulating in serum is a candidate molecular biomarker for malignant tumors. Unlike uniformly truncated released from apoptotic cells, dead cancer cells varies size. Serum integrity, the ratio of longer fragments to total DNA, may be clinically useful detecting breast progression. Patients and Methods samples 51 healthy females 83 with primary cancers (eight American Joint Committee on Cancer stage 0, 24 I, 27 II, 21 III, three IV) were assessed preoperatively....
Toll-like receptors (TLRs) play a crucial role in the innate immune response and subsequent induction of adaptive responses against microbial infection or tissue injury. Recent findings show that functional TLRs are expressed not only on cells but also cancer cells. an active carcinogenesis tumor progression during chronic inflammation involves microenvironment. Damage-associated molecular patterns (DAMPs) derived from injured normal epithelial necrotic appear to be present at significant...
The mechanisms underlying hypermethylation of tumor-suppressor gene promoters in cancer is not well understood. Here, we report that lysine acetylation the oncogenic transcription factor STAT3 elevated tumors. We also show genetically altering at Lys685 reduces tumor growth, which accompanied by demethylation and reactivation several genes. Moreover, mutating disrupts DNA methyltransferase 1–STAT3 interactions cultured cells These observations are confirmed treatment with an inhibitor,...
Although targeting oncogenic mutations in the BRAF serine/threonine kinase with small molecule inhibitors can lead to significant clinical responses melanoma, it fails eradicate tumors nearly all patients. Successful therapy will be aided by identification of intrinsic mechanisms that protect tumor cells from death. Here, we used a bioinformatics approach identify drug-able, “driver” oncogenes restricted versus normal tissues. Applying this method 88 short-term melanoma cell cultures, show...
Endocrine therapy resistance frequently develops in estrogen receptor positive (ER+) breast cancer, but the underlying molecular mechanisms are largely unknown. Here, we show that 3-dimensional (3D) chromatin interactions both within and between topologically associating domains (TADs) change ER+ endocrine-resistant cancer cells differential enriched for resistance-associated genetic variants at CTCF-bound anchors. Ectopic preferentially active enhancers promoters ER binding sites,...
Abstract The hypoxic tumor microenvironment has been implicated in immune escape, but the underlying mechanism remains elusive. Using an vitro culture system modeling human T cell dysfunction and exhaustion triple-negative breast cancer (TNBC), we find that hypoxia suppresses effector gene expression, including NK cells, resulting resistance to immunotherapy. We demonstrate hypoxia-induced factor 1α (HIF1α) interaction with HDAC1 concurrent PRC2 dependency causes chromatin remolding...
PURPOSE This study was performed to evaluate the potential of specific mRNA markers detect micrometastases by reverse-transcriptase polymerase chain reaction (RT-PCR) and Southern blot analysis sentinel lymph nodes (SNs) blood from patients with breast cancer. PATIENTS AND METHODS We assessed specificity carcinoembryonic antigen (CEA), cytokeratin-19 (CK-19), CK-20, gastrointestinal tumor-associated antigen-733.2 (GA733.2), mucin-1 (MUC-1) in healthy donors (n = 13) without cancer 3) RT-PCR...
PURPOSE The objective of the study was to develop a sensitive multimarker polymerase chain reaction (PCR) assay detect circulating melanoma cells in patient blood. rationale that malignant is heterogeneous regards antigen expression. PATIENTS AND METHODS A PCR uses four melanoma-associated gene markers (tyrosinase, p97, MUC18, and MAGE-3) developed. Sensitivity specificity for individual were assessed using 10 cell lines peripheral-blood lymphocytes (PBL) from 39 normal volunteers as...
Cell-free DNA circulating in blood is a candidate biomarker for malignant tumors. Unlike uniformly truncated released from apoptotic nondiseased cells, dead cancer cells varies size. We developed novel method to measure the ratio of longer shorter fragments (DNA integrity) serum as potential patients with colorectal (CRC) or periampullary cancers (PACs).Sera 32 CRC (3 stage I, 14 II, 6 III, and 9 IV patients), 19 PACs (2 1 7 51 healthy volunteers were assessed by quantitative real-time PCR...
PURPOSE: Detection of micrometastases in the regional tumor-draining lymph nodes is critical for accurate staging and prognosis melanoma patients. We hypothesized that a multiple-mRNA marker (MM) reverse transcriptase–polymerase chain reaction (RT-PCR) assay would improve detection occult metastases sentinel node (SN), compared with hematoxylin eosin (H&E) staining immunohistochemistry (IHC), MM expression predictive disease relapse. PATIENTS AND METHODS: Seventy-two consecutive patients...
In Brief Objective: Lymphatic mapping and sentinel lymphadenectomy (LM/SL) have been applied to virtually all solid neoplasms since our original description of LM/SL for melanoma. Our objectives were determine the diagnostic therapeutic utility LM/SL, investigate carbon dye microanatomy lymphatic flow within node (SN), prognostic accuracy molecular assessment SN. Methods: Since 1985, 1599 patients with AJCC Stage I/II melanoma treated by at institution 4590 wide excision (WE) without nodal...