A5090998899- Inflammasome and immune disorders
- Immune Response and Inflammation
- interferon and immune responses
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Inflammatory mediators and NSAID effects
- Single-cell and spatial transcriptomics
- Chemical Synthesis and Analysis
- SARS-CoV-2 and COVID-19 Research
- Fluorine in Organic Chemistry
- Tryptophan and brain disorders
- IL-33, ST2, and ILC Pathways
- Helicobacter pylori-related gastroenterology studies
- Heme Oxygenase-1 and Carbon Monoxide
- Advanced Drug Delivery Systems
- Cancer-related molecular mechanisms research
- Receptor Mechanisms and Signaling
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- RNA regulation and disease
- Drug Solubulity and Delivery Systems
- MicroRNA in disease regulation
University of Washington
1981-2025
Institute for Systems Biology
2014-2025
Seattle University
2020-2021
InSysBio (Russia)
2018-2021
Himalayan University
2020
North Seattle College
2020
National Institute of Immunology
2006-2018
Anna University, Chennai
2018
Bharathidasan University
2018
National Institutes of Health
2011-2015
Post-acute sequelae of COVID-19 (PASC) represent an emerging global crisis. However, quantifiable risk factors for PASC and their biological associations are poorly resolved. We executed a deep multi-omic, longitudinal investigation 309 patients from initial diagnosis to convalescence (2-3 months later), integrated with clinical data patient-reported symptoms. resolved four PASC-anticipating at the time diagnosis: type 2 diabetes, SARS-CoV-2 RNAemia, Epstein-Barr virus viremia, specific...
We present an integrated analysis of the clinical measurements, immune cells, and plasma multi-omics 139 COVID-19 patients representing all levels disease severity, from serial blood draws collected during first week infection following diagnosis. identify a major shift between mild moderate disease, at which point elevated inflammatory signaling is accompanied by loss specific classes metabolites metabolic processes. Within this stressed environment multiple unusual cell phenotypes emerge...
Mendelian analysis of disorders immune regulation can provide insight into molecular pathways associated with host defense and tolerance.We identified three families a dominantly inherited complex cold-induced urticaria, antibody deficiency, susceptibility to infection autoimmunity. Immunophenotyping methods included flow cytometry, serum immunoglobulins autoantibodies, lymphocyte stimulation, enzymatic assays. Genetic studies linkage analysis, targeted Sanger sequencing, next-generation...
Regulatory T cells (Treg) are key players in maintaining immune homeostasis but have also been shown to regulate responses against infectious pathogens. Therefore, Treg a promising target for modulating vaccines improve their efficacy. Using viral vector system, we found that act on the developing response early postinfection by reducing extent of dendritic cell costimulatory molecule expression. Due this change and lower IL-2 production results, substantial fraction CD8(+) effector lose...
Lyme disease (LD) is growing in incidence, with nearly 500,000 cases diagnosed annually the United States. Despite treatment, some patients experience persistent symptoms. The immune mechanisms underlying LD remain poorly understood. We conducted a multiomic longitudinal analysis of 49 and matched controls, integrating plasma proteomics, metabolomics, PBMC immunophenotyping, meta-analysis skin lesions. identified compartmentalized responses acute LD, coordinated alterations circulating...
Antibody-recruiting protein-catalyzed capture agent (AR-PCCs) are a new class of all-synthetic and highly targeted antibiotics that recruit endogenous immune responses to eliminate drug-resistant microbes.
Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive response map spanning 454 proteins 847 metabolites plasma integrated with single-cell multi-omic assays of PBMCs which whole transcriptome, 192 surface proteins, T B cell receptor sequence were co-analyzed within the context clinical measures from 50 COVID19 patient samples. Our study reveals novel cellular subpopulations, such as...
Significance While the effect of bacterial molecules on host immune system is well studied, how factors affect expression less appreciated. Here we uncover impact inflammasome activation and type 1 interferon flagellin. Flagellin induces NLRC4 inflammasome-mediated pyroptosis causing clearance Salmonella . We show that also produces lysophospholipids which increase flagellin by early in infection. further demonstrate as infection progresses, IFN inhibits lysophospholipid synthesis, resulting...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntramolecular .alpha.-amidoalkylation of an olefin for the synthesis a useful prostaglandin intermediateTsung-Tee Li, Patricia Lesko, Robert H. Ellison, N. Subramanian, and John FriedCite this: J. Org. Chem. 1981, 46, 1, 111–115Publication Date (Print):January 1981Publication History Published online1 May 2002Published inissue 1 January 1981https://pubs.acs.org/doi/10.1021/jo00314a024https://doi.org/10.1021/jo00314a024research-articleACS...
Angiotensin-converting enzyme inhibitors (ACEi) and angiotensin-II receptor blockers (ARB), the most commonly prescribed antihypertensive medications, counter renin-angiotensin-aldosterone system (RAAS) activation via induction of angiotensin-converting 2 (ACE2) expression. Considering that ACE2 is functional for SARS-CoV-2 entry into host cells, association ACEi ARB with COVID-19 outcomes needs thorough evaluation.We conducted retrospective analyses using both unmatched propensity score...
A disproportionately large response is induced by small increases in the abundance of a pathogen sensor.
Host immune responses play central roles in controlling SARS-CoV2 infection, yet remain incompletely characterized and understood. Here, we present a comprehensive response map spanning 454 proteins 847 metabolites plasma integrated with single-cell multi-omic assays of 221,748 PBMCs which whole transcriptome, 192 surface proteins, T B cell receptor sequence were analyzed within the context clinical measures from 50 COVID19 patient samples. Our study reveals novel cellular subpopulations,...