A5087395773- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- CRISPR and Genetic Engineering
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Axon Guidance and Neuronal Signaling
- Advanced Fluorescence Microscopy Techniques
- Microtubule and mitosis dynamics
- Congenital heart defects research
- Neural dynamics and brain function
- Cellular transport and secretion
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Protist diversity and phylogeny
- Olfactory and Sensory Function Studies
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Hemoglobinopathies and Related Disorders
- Nuclear Structure and Function
- Neurobiology and Insect Physiology Research
Institut de la Vision
2020-2025
Inserm
2016-2025
Sorbonne Université
2015-2025
Centre National de la Recherche Scientifique
2016-2025
Institut Curie
2014-2024
Université Paris Sciences et Lettres
2016-2024
Neurosciences Paris-Seine
2023
Institut de Biologie Paris-Seine
2023
Laboratoire de Biologie du Développement
2018-2022
Génétique et biologie du développement
2016-2022
Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination microscopy (SPIM) generate multidimensional images samples up a few millimeters in size. The system combines two-dimensional with orthogonal camera-based detection achieve high-resolution, optically sectioned throughout the sample, minimal photodamage at speeds capable capturing transient phenomena. used...
Sequence-specific nucleases like TALENs and the CRISPR/Cas9 system have greatly expanded genome editing possibilities in model organisms such as zebrafish. Both systems recently been used to create knock-out alleles with great efficiency, also successfully employed knock-in of DNA cassettes at defined loci via homologous recombination (HR). Here we report CRISPR/Cas9-mediated into zebrafish a very high rate by homology-independent double-strand break (DSB) repair pathways. After co-injection...
Abstract Throughout vertebrates, cerebrospinal fluid-contacting neurons (CSF-cNs) are ciliated cells surrounding the central canal in ventral spinal cord. Their contribution to modulate locomotion remains undetermined. Recently, we have shown CSF-cNs by directly projecting onto locomotor pattern generators (CPGs), but sensory modality these convey circuits and their relevance innate remain elusive. Here, demonstrate vivo that form an intraspinal mechanosensory organ detects bending. By...
Expression of halorhodopsin (NpHR), a light-driven microbial chloride pump, enables optical control membrane potential and reversible silencing targeted neurons. We generated transgenic zebrafish expressing enhanced NpHR under the Gal4/UAS system. Electrophysiological recordings showed that eNpHR stimulation effectively suppressed spiking single neurons in vivo. Applying light through thin optic fibers positioned above head semi-restrained larva enabled us to target groups simultaneously...
The optic tectum of zebrafish is involved in behavioral responses that require the detection small objects. superficial layers tectal neuropil receive input from retinal axons, while its deeper convey processed information to premotor areas. Imaging with a genetically encoded calcium indicator revealed deep layers, as well dendrites single neurons, are preferentially activated by visual stimuli. This spatial filtering relies on GABAergic interneurons (using neurotransmitter γ-aminobutyric...
The optokinetic response (OKR) to a visual stimulus moving at constant velocity consists of series two alternating components, slow phase, during which the eyes follow stimulus, and quick resets begin new cycle. phases OKR resemble saccades observed free viewing. It is unclear what extent premotor circuitry underlying these types jerky, conjugate eye movements conserved among vertebrates. Zebrafish ( Danio rerio ) larvae, broadly expressing halorhodopsin (NpHR) or channelrhodopsin-2 (ChR2)...
Over 90 years ago, Kolmer and Agduhr identified spinal cerebrospinal fluid-contacting neurons (CSF-cNs) based on their morphology location within the cord. In more than 200 vertebrate species, they observed ciliated around central canal that extended a brush of microvilli into fluid (CSF). Although is suggestive primitive sensory cell, function cord remains unknown. The identification specific molecular markers for these in vertebrates would benefit investigation physiological roles. PKD2L1,...
Abstract Sickle cell disease and β-thalassemia affect the production of adult β-hemoglobin chain. The clinical severity is lessened by mutations that cause fetal γ-globin expression in life (i.e., hereditary persistence hemoglobin). Mutations clustering ~200 nucleotides upstream HBG transcriptional start sites either reduce binding LRF repressor or recruit KLF1 activator. Here, we use base editing to generate a variety −200 region promoters, including potent combinations four eight...
Two-photon, single-cell resolution optogenetics based on holographic light-targeting approaches enables the generation of precise spatiotemporal neuronal activity patterns and thus a broad range experimental applications, such as high throughput connectivity mapping probing neural codes for perception. Yet, current limit tuning relative spiking time distinct cells to few milliseconds, achievable number targets 100-200, depending working depth. To overcome these limitations expand...
Progressive inherited retinal degenerations (IRDs) affecting rods and cones are clinically genetically heterogeneous can lead to blindness with limited therapeutic options. The major gene defects have been identified in subjects of European Asian descent only few reports North African descent. Genome, targeted next-generation, Sanger sequencing was applied cohort ∼4000 IRDs cases. Expression analyses were performed including Chip-seq database analyses, on human-derived organoids (ROs),...