A5005624697- Cancer Cells and Metastasis
- Caveolin-1 and cellular processes
- Cellular Mechanics and Interactions
- Nanoplatforms for cancer theranostics
- Extracellular vesicles in disease
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Barrier Structure and Function Studies
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Nanoparticle-Based Drug Delivery
- Micro and Nano Robotics
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Galectins and Cancer Biology
- Parathyroid Disorders and Treatments
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Cancer Research and Treatments
- Platelet Disorders and Treatments
- Immunotherapy and Immune Responses
Université de Strasbourg
2016-2025
Inserm
2016-2025
La Ligue Contre le Cancer
2021-2025
Institut de Cancérologie Strasbourg
2024
Immuno-Rhumathologie moléculaire
2015-2024
Institut Polytechnique de Bordeaux
2024
Centre National de la Recherche Scientifique
2008-2022
Institut de Chimie
2022
Institut de Chimie et Procédés pour l'Energie, l'Environnement et la Santé
2022
Institut de Physique et Chimie des Matériaux de Strasbourg
2022
Exosomes are secreted vesicles arising from the fusion of multivesicular bodies (MVBs) with plasma membrane. Despite their importance in various processes, molecular mechanisms controlling formation and release remain unclear. Using nematodes mammary tumor cells, we show that Ral GTPases involved exosome biogenesis. In Caenorhabditis elegans, RAL-1 localizes at surface secretory MVBs. A quantitative electron microscopy analysis RAL-1–deficient animals revealed is both MVB These functions do...
The pattern of blood flow has long been thought to play a significant role in vascular morphogenesis, yet the flow-sensing mechanism that is involved at early embryonic stages, when forces are low, remains unclear. It proposed endothelial cells use primary cilia sense flow, but this never tested vivo. Here we show, by noninvasive, high-resolution imaging live zebrafish embryos, progressively deflect onset and deflection angle correlates with calcium levels cells. We demonstrate alterations...
Abstract Despite abundant evidence demonstrating that platelets foster metastasis, anti-platelet agents have low therapeutic potential due to the risk of hemorrhages. In addition, whether can regulate metastasis at late stages disease remains unknown. this study, we subject syngeneic models various thrombocytopenic regimes show provide a biphasic contribution metastasis. While potent intravascular binding tumor cells efficiently promotes further support outgrowth established metastases via...
Rho/ROCK signaling and caveolin-1 (Cav1) are implicated in tumor cell migration metastasis; however, the underlying molecular mechanisms remain poorly defined. Cav1 was found here to be an independent predictor of decreased survival breast rectal cancer significantly associated with presence distant metastasis for colon patients. promotes by regulating focal adhesion (FA) dynamics through tyrosine (Y14) phosphorylation Cav1. Phosphorylated is localized protrusive domains cells dependent on...
Macromolecular complexes exhibit reduced diffusion in biological membranes; however, the physiological consequences of this characteristic plasma membrane domain organization remain elusive. We report that competition between galectin lattice and oligomerized caveolin-1 microdomains for epidermal growth factor (EGF) receptor (EGFR) recruitment regulates EGFR signaling tumor cells. In mammary cells deficient Golgi β1,6N-acetylglucosaminyltransferase V (Mgat5), a reduction binding to allows an...
Oncogenic signaling stimulates the dynamic remodeling of actin microfilaments and substrate adhesions, essential for cell spreading motility.Transformation is associated with increased expression 1,6GlcNAcbranched N-glycans, products Golgi 1,6-acetylglucosaminyltransferase V (Mgat5) favored ligand galectins.Herein we report that fibronectin fibrillogenesis fibronectin-dependent are deficient in Mgat5 ؊/؊ mammary epithelial tumor cells inhibited ؉/؉ by blocking N-glycan processing...
Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together pY14Cav1 stabilize adhesion kinase (FAK) within FAs, thereby promotes FA disassembly turnover. Expression of the Mgat5/galectin lattice alone induces FAs cell spreading. However, FAK stabilization also requires expression pY14Cav1. In...
Shwachman-Diamond syndrome (SDS) (OMIM #260400) is a rare inherited bone marrow failure (IBMFS) that primarily characterized by neutropenia and exocrine pancreatic insufficiency. Seventy-five to ninety percent of patients have compound heterozygous loss-of-function mutations in the Shwachman-Bodian-Diamond (sbds) gene. Using trio whole-exome sequencing (WES) an sbds-negative SDS family candidate gene additional SBDS-negative cases or molecularly undiagnosed IBMFS cases, we identified 3...
Highlights•Fast-moving cells in 3D matrix establish low membrane tension at the rear•Caveolae form response to and recruit GEF Ect2•Ect2 activates RhoA promote F-actin organization rear retraction•Positive feedback between contractility reinforces retractionSummaryIn development, wound healing, cancer metastasis, vertebrate move through interstitial matrix, responding chemical physical guidance cues. Protrusion cell front has been extensively studied, but retraction phase of migration cycle...
Intravital microscopy provides dynamic understanding of multiple cell biological processes, but its limited resolution has so far precluded structural analysis. Because it is difficult to capture rare and transient events, only few attempts were made observe specific developmental pathological processes in animal models by electron microscopy. The multimodal correlative approach that we propose here combines intravital microscopy, microscopic X-ray computed tomography three-dimensional It...
Fluorescent polymer nanoparticles for long-term labeling and tracking of living cells with any desired color code are developed. They built from biodegradable poly(lactic-co-glycolic acid) loaded cyanine dyes (DiO, DiI, DiD) the help bulky fluorinated counterions, which minimize aggregation-caused quenching. At single particle level, these particles ≈20-fold brighter than quantum dots similar color. Due to their identical 40 nm size surface properties, endocytosed equally well by cells....
Lipid nanocarriers are considered as promising candidates for drug delivery and cancer targeting because of their low toxicity, biodegradability capacity to encapsulate drugs and/or contrasting agents. However, biomedical applications currently limited a poor understanding integrity in vivo. To address this problem, we report on fluorescent nano-emulsion droplets 100nm size encapsulating lipophilic near-infrared cyanine 5.5 7.5 dyes with help bulky hydrophobic counterion tetraphenylborate....
Cancer metastasis is a process whereby primary tumor spreads to distant organs. We have demonstrated previously that blood flow controls the intravascular arrest of circulating cells (CTCs) through stable adhesion endothelial cells. now aim define contribution cell potential and identify receptors at play. Early mediated by formation weak adhesion, depending on CD44 integrin αvβ3. Stabilization this uses α5β1-dependent adhesions with higher strength, which allows CTCs stop in vascular...