A5057462377- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Photochemistry and Electron Transfer Studies
- RNA Research and Splicing
- Molecular Sensors and Ion Detection
- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Photochromic and Fluorescence Chemistry
- Spectroscopy and Quantum Chemical Studies
- Click Chemistry and Applications
- Photoreceptor and optogenetics research
- Porphyrin and Phthalocyanine Chemistry
- Nanoplatforms for cancer theranostics
- Analytical Chemistry and Sensors
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Radical Photochemical Reactions
- Virus-based gene therapy research
Centre National de la Recherche Scientifique
2016-2025
Laboratoire de Biophotonique et Pharmacologie
2016-2025
Université de Strasbourg
2016-2025
Pharmac
2014-2020
Bipar
2014-2016
Institute of Pharmacology
2014
Faculty (United Kingdom)
2014
École Normale Supérieure Paris-Saclay
2013
Laboratoire de Biologie et Pharmacologie Appliquée
2013
University of Basel
2012
Cholesterol and sphingomyelin form together a highly ordered membrane phase, which is believed to play important biological functions in plasma membranes of mammalian cells. Since present mainly at the outer leaflet cell membranes, monitoring its lipid order requires molecular probes capable bind specifically this exhibit negligibly slow flip-flop. In work, such probe was developed by modifying solvatochromic fluorescent dye Nile Red with an amphiphilic anchor group. To evaluate flip-flop...
Herein, we developed the first ratiometric fluorescent probe for apoptosis detection. This incorporates selectively into outer leaflet of cell plasma membrane and senses loss asymmetry occurring during early steps apoptosis. The high specificity to membranes was achieved by introduction a anchor, composed zwitterionic group long (dodecyl) hydrophobic tail. fluorescence reporter this is 4'-(diethylamino)-3-hydroxyflavone, which exhibits excited-state intramolecular proton transfer (ESIPT),...
In a search for environmentally sensitive (solvatochromic) dyes with superior properties, we extended the electronic conjugation of one best solvatochromic dyes, Prodan, by substituting its naphthalene core fluorene. The newly synthesized fluorene derivatives bearing strong electron-donor (dialkylamino) and -acceptor (carbonyl) groups at 2 7 positions showed red-shifted absorption (close to 400 nm), twice as large coefficient (43 000 M−1 cm−1), manifold larger two-photon cross section (∼400...
Plasmodesmata (PD) are essential but poorly understood structures in plant cell walls that provide symplastic continuity and intercellular communication pathways between adjacent cells thus play fundamental roles development pathogenesis. Viruses encode movement proteins (MPs) modify these tightly regulated pores to facilitate their spread from cell. The most striking of modifications is observed for groups viruses whose MPs form tubules assemble PDs through which virions transported...
Dye-loaded lipid nano-droplets present an attractive alternative to inorganic nanoparticles, as they are composed of non-toxic biodegradable materials and easy prepare. However, achieve high fluorescence brightness, the have be heavily loaded with dyes avoiding self-quenching release (leakage) encapsulated from in biological media. In work, we designed highly lipophilic fluorescent derivatives 3-alkoxyflavone (F888) Nile Red (NR668) that can core stable nano-emulsion droplets at...
Abstract Imaging lipid organization in cell membranes requires advanced fluorescent probes. Here, we show that a recently synthesized push-pull pyrene (PA), similarly to popular probe Laurdan, changes the emission maximum as function of order, but outperforms it by spectroscopic properties. In addition red-shifted absorption compatible with common 405 nm diode laser, PA shows higher brightness and much photostability than Laurdan apolar membrane environments. Moreover, is two-photon...
Fraternal twins: Oligoureas and γ-peptides are isosteric, quasi-isostructural helical foldamers endowed with distinct biomolecular recognition properties. Combination of the two backbones to generate urea/amide hybrids (see picture) was found give more potent yet less cytotoxic antimicrobial foldamers.
Supramolecular organization of enzymes is proposed to orchestrate metabolic complexity and help channel intermediates in different pathways. Phenylpropanoid metabolism has direct up 30% the carbon fixed by plants biosynthesis lignin precursors. Effective coupling pathway thus seems be required. Subcellular localization, mobility, protein–protein, protein–membrane interactions four consecutive around main branch point leading precursors was investigated leaf tissues Nicotiana benthamiana...
Lipid droplets (LDs) are organelles composed of a lipid core surrounded by phospholipid monolayer. Lately, LDs have attracted considerable attention due to recent studies demonstrating their role in variety physiological processes as well diseases. Herein we synthesized push–pull molecule named DAF (Dimethyl Aniline Furaldehyde) that possesses strong positive solvatochromism emission 119 nm from toluene methanol. Its impressive fluorogenic properties water oil (2000-fold) its high quantum...
Ultrasmall polymer nanoparticles are rapidly gaining importance as nanocarriers for drugs and contrast agents. Here, a straightforward modular approach to efficiently loaded stable sub-20-nm particles is developed. In order obtain ultrasmall nanoparticles, we investigated the influence of one two charged groups per chain on size obtained by nanoprecipitation. Negatively carboxylate sulfonate or positively trimethylammonium were introduced into polymers poly(d,l-lactide-co-glycolide) (PLGA),...
Lipid nanocarriers are considered as promising candidates for drug delivery and cancer targeting because of their low toxicity, biodegradability capacity to encapsulate drugs and/or contrasting agents. However, biomedical applications currently limited a poor understanding integrity in vivo. To address this problem, we report on fluorescent nano-emulsion droplets 100nm size encapsulating lipophilic near-infrared cyanine 5.5 7.5 dyes with help bulky hydrophobic counterion tetraphenylborate....
Piling up excited states to reach upconversion (UC) is severely restricted by vibrational quenching mechanisms, especially when one looks at discrete molecular entities in solution. By carefully controlling the supramolecular assembly processes resulting from strong electrostatic interactions between negatively charged Yb complexes and Tb3+ cations aqueous solutions, we engineered formation of heteropolynuclear [(YbL)2Tb x] compositions ( x = 1 2). These edifices display a phenomenon...
A rational design of amphiphilic squaraine dyes tunes cell entry, allowing for selective far-red/near-infrared imaging plasma membrane <italic>vs.</italic> endoplasmic reticulum at 1 nM probe concentration.
Picosecond time-resolved fluorescence spectroscopy has been applied to the studies of excited-state intramolecular proton transfer (ESIPT) dynamics in two 4'-(dialkylamino)-3-hydroxyflavone derivatives (unsubstituted and substituted at 6-position) ethyl acetate dichloromethane. In all studied cases, decay kinetics both short-wavelength normal (N*) long-wavelength tautomer (T*) bands can be characterized by same lifetime components, which are constant over wavelength range emission. meantime,...
A straightforward method for the synthesis of original 4,4-dialkoxy- or 4,4-diaryloxy-diaza-s-indacenes (BODIPY) derivatives obtained by treatment BODIPY 1 with various alcohols in presence AlCl3 is described. The novel compounds are characterized spectroscopic properties similar to those parent 1, absorption and emission spectra band shapes, high molar coefficients (ελmax ≈ 80 000 M-1 cm-1), most them fluorescence quantum yields (Φexp from 0.52 0.71). Among all new synthesized, dye 2h...
The principle of electrochromic modulation excited-state intramolecular proton-transfer reaction was applied for the design fluorescence probes with high two-color sensitivity to dipole potential, Ψ d , in phospholipid bilayers. We report on effect variation excitation and spectra two new 3-hydroxyflavone probes, which possess opposite orientations fluorescent moiety lipid bilayer. potential bilayer modulated by addition 6-ketocholestanol or phloretin substitution dimyristoyl...
The spectroscopic behavior of the 4'-dialkylamino-3-hydroxyflavones in protic environments is very unusual. Previous studies showed that contrast to other solvatochromic dyes containing carbonyl group (coumarins, Nile Red, PRODAN, etc.), their Stokes shift does not increase on formation intermolecular H-bonds with solvents. present steady-state and time-resolved show ground-state equilibrium between H-bonded non-H-bonded forms this derivative mixed solvents changed significantly when dye...