A5070213528- HIV Research and Treatment
- Lipid Membrane Structure and Behavior
- Nanoparticle-Based Drug Delivery
- Advanced Fluorescence Microscopy Techniques
- RNA Research and Splicing
- Hydrocarbon exploration and reservoir analysis
- Petroleum Processing and Analysis
- RNA Interference and Gene Delivery
- Lanthanide and Transition Metal Complexes
- Nanoplatforms for cancer theranostics
- Cytomegalovirus and herpesvirus research
- Neuroscience and Neuropharmacology Research
- HIV/AIDS drug development and treatment
- Molecular Sensors and Ion Detection
- Luminescence and Fluorescent Materials
- Oil Spill Detection and Mitigation
- RNA and protein synthesis mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Electrohydrodynamics and Fluid Dynamics
- Mass Spectrometry Techniques and Applications
- Carbon and Quantum Dots Applications
- Supramolecular Chemistry and Complexes
- Innovative Microfluidic and Catalytic Techniques Innovation
- MRI in cancer diagnosis
- Photodynamic Therapy Research Studies
Centre National de la Recherche Scientifique
2015-2025
Université de Strasbourg
2014-2025
Laboratoire de Biophotonique et Pharmacologie
2014-2025
Institut de génétique et de biologie moléculaire et cellulaire
2012-2013
Inserm
2012-2013
Dye-loaded lipid nano-droplets present an attractive alternative to inorganic nanoparticles, as they are composed of non-toxic biodegradable materials and easy prepare. However, achieve high fluorescence brightness, the have be heavily loaded with dyes avoiding self-quenching release (leakage) encapsulated from in biological media. In work, we designed highly lipophilic fluorescent derivatives 3-alkoxyflavone (F888) Nile Red (NR668) that can core stable nano-emulsion droplets at...
In this study, we investigated the role of chemical nature oil droplet core nano-emulsions used as contrast agents for X-ray imaging on their pharmacokinetics and biodistribution. To end, formulated PEGylated with two iodinated oils (i.e., monoglyceride castor oil) compared them another nano-emulsion based vitamin E. By using dynamic light scattering transmission electron microscopy, three were found to exhibit comparable morphologies, size, surface composition. Furthermore, they shown be...
A rotor-based method to measure oil viscosity in nano-scaled lipid domains.
Pulsatile flow is a universal feature of the blood circulatory system in vertebrates and can lead to diseases when abnormal. In embryo, forces stimulate vessel remodeling stem cell proliferation. At these early stages, vessels lack muscle cells, heart valveless Reynolds number (Re) low, few details are available regarding mechanisms controlling pulses propagation developing vascular network. Making use recent advances optical-tweezing probing approaches, fast imaging elastic-network viscous...
Photodynamic therapy (PDT) is a photochemistry-based medical treatment combining light at specific wavelength and photosensitizer (PS) in the presence of oxygen. Application PDT as conventional limited clearly approval clinics new PS challenging. The selective accumulation targeted malignant cells paramount importance to reduce side effects that are typical current worldwide approved PS. Here we report series aniline- iodine-substituted BODIPY derivatives (1-3) promising lysosome-targeting...
Double emulsions are very attractive systems for many reasons; the most important of these their capacity to encapsulate hydrophilic and lipophilic molecules simultaneously in a single particle potentiality protect fragile from continuous phase. represent technology that is widely present down micrometer scale; however, double nanoemulsions, with new potential applications as nanomedicines or diagnosis agents, currently significant challenge. In this study, we propose an original two-step...
The nucleocapsid protein (NCp7) of the Human immunodeficiency virus type 1 (HIV-1) is a small basic containing two zinc fingers. About 2000 NCp7 molecules coat genomic RNA in HIV-1 virion. After infection target cell, viral core enters into cytoplasm, where chaperones reverse transcription proviral DNA. As consequence their much lower affinity for double-stranded DNA as compared to single-stranded RNAs, are thought be released cytoplasm and nucleus infected cells late steps transcription....
Noninvasive diagnostic by imaging combined with a contrast agent (CA) is now the most used technique to get insight into human bodies. X-ray and magnetic resonance (MRI) are widely technologies providing complementary results. Nowadays, it seems clear that bimodal CAs could be an emerging approach increase patient compliance, accessing different modalities single CA injection. Owing versatile designs, targeting properties, high payload capacity, nanocarriers considered as viable solution...
Nanoemulsions (NEs) are biocompatible lipid nanoparticles composed of an oily core stabilized by a surfactant shell. It is acknowledged that the surface decoration with poly(ethylene glycol), through use nonionic surfactants, confers high stealth in biological medium reduced nonspecific interactions. Tracking individual NE fluorescence microscopy techniques would lead to better understanding their behavior cells and thus require development bright single particles enhanced photostability....
Abstract Graphene‐based 2D nanomaterials exhibit unique physicochemical, electric, and optical properties that facilitate applications in a wide range of fields including material science, electronics, biotechnology. Recent studies have shown graphene oxide (GO) reduced (rGO) antimicrobial effects on bacteria viruses. While the bactericidal activity graphene‐based is related to mechanical oxidative damage bacterial membranes, their antiviral has been less explored. Currently available...
Abstract Fluorescence microscopy imaging of individual HIV-1 viruses necessitates a specific labeling viral structures that minimally perturbs the infection process. Herein, we used pseudoviruses containing NCp7 fused to tetracystein (TC) tag, labeled by biarsenical fluorescein derivative (FlAsH) quantitatively monitor protein concentration in cores during early stages infection. Single particle with defined ratios TC-tagged non tagged proteins, together theoretical modeling energy transfer...
ABSTRACT The human immunodeficiency virus-1 (HIV-1) nucleocapsid protein (NCp7) is a nucleic acid chaperone with two highly conserved zinc fingers. To exert its key roles in the viral cycle, NCp7 interacts several host proteins. Among them, NoL12 (hNoL12) was previously identified genome wide screens as potential partner of NCp7. hNoL12 25 kDa nucleolar RNA-binding implicated 5′end processing ribosomal RNA nucleolus and thus assembly maturation ribosomes. In this work, we confirmed...
Appropriate surface ligands are required for tuning the physicochemical and photophysical properties of nanoclusters (NCs). These especially critical passivating small (CdSe)33,34 NCs where majority atoms at NC surface. In this study, triphenylphosphine (TPP), trioctylphosphine (TOP) tris(pentafluorophenyl)phosphine (TPFP) have been tested as capping agents alkylamine-coated CdSe NCs. TPP TOP compounds found to increase quantum yield photoluminescence (PL) from 0.15% 0.6% 0.53%,...